Investigation of the protective effect of chitosan against arsenic-induced nephrotoxicity and oxidative damage in rat kidney tissue

• Autorzy: Irak K. , Celik O.Y. , Bolacalı M. , Tufan T. , Ozcan C. , Yıldırım S. , Bolat I.
• Języki publikacji: EN

Abstrakty

EN

Arsenic is an important metalloid that can cause poisoning in humans and domestic animals. Exposure to arsenic causes cell damage, increasing the production of reactive oxygen species. Chitosan is a biopolymer obtained by deacetylation of chitin with antioxidant and metal ion chelating properties. In this study, the protective effect of chitosan on arsenic-induced nephrotoxicity and oxidative damage was investigated. 32 male Wistar-albino rats were divided into 4 groups of 8 rats each as control group (C), chitosan group (CS group), arsenic group (AS group), and arsenic+chitosan group (AS+CS group). The C group was given distilled water by oral gavage, the AS group was given 100 ppm/day Na-arsenite ad libitum with drinking water, the CS group was given 200 mg/kg/day chitosan dissolved in saline by oral gavage, the AS+CS group was given 100 ppm/day Na-arsenite ad libitum with drinking water and 200 mg/kg/day chitosan dissolved in saline by oral gavage for 30 days. At the end of the 30-day experimental period, 90 mg/kg ketamine was administered intraperitoneally to all rats, and blood samples and kidney tissues were collected. Urea, uric acid, creatinine, P, Mg, K, Ca, Na, Cystatin C (CYS-C), Neutrophil Gelatinase Associated Lipocalin (NGAL) and Kidney Injury Molecule 1 (KIM-1) levels were measured in serum samples. Malondialdehyde (MDA), Glutathione (GSH), Catalase (CAT) and Superoxide dismutase (SOD) levels in the supernatant obtained from kidney tissue were analyzed by ELISA method. Compared with AS group, uric acid and creatinine levels of the AS+CS group were significantly decreased (p<0.001), urea, KIM-1, CYS-C, NGAL, and MDA levels were numerically decreased and CAT, GSH, and SOD levels were numerically increased (p>0.05). In conclusion, based on both biochemical and histopathological-immunohistochemical-immunofluorescence findings, it can be concluded that chitosan attenuates kidney injury and protects the kidney.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2024
• Tom: 27
• Numer: 1
• Opis fizyczny: p.95-105,fig.,ref.

Twórcy

autor

Irak K.

• Department of Biochemistry, Faculty of Veterinary Medicine, Siirt University, Siirt, Turkey

autor

Celik O.Y.

• Department of Internal Medicine, Faculty of Veterinary Medicine, Siirt University, Siirt, Turkey

autor

Bolacalı M.

• Kırsehir Ahi Evran University, Faculty of Medicine, Department of Biostatistics and Medical Informatics, Kirsehir, Turkey

autor

Tufan T.

• Department of Animal Nutrition and Nutritional Disease, Faculty of Veterinary Medicine, Siirt University, Siirt, Turkey

autor

Ozcan C.

• Department of Animal Nutrition and Nutritional Disease, Faculty of Veterinary Medicine, Siirt University, Siirt, Turkey

autor

Yıldırım S.

• Department of Pathology, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey

autor

Bolat I.

• Department of Pathology, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey

Identyfikatory

DOI

10.24425/pjvs.2024.149339