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2013 | 35 | 03 |

Tytuł artykułu

Nodularia spumigena extract induces upregulation of mitochondrial respiratory chain complexes in spinach (Spinacia oleracea L.)

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Nodularin, a cyclic hepatotoxic pentapeptide produced by the nitrogen-fixing cyanobacterium Nodularia spumigena, induces oxidative stress in various organisms including higher plants and algae. We have monitored the physiological consequences of N. spumigena AV1 extract exposure on terrestrial plants, specifically focusing on the mitochondrial function of Spinacia oleracea L. Our results show that exposure of the plants to the nodularin-containing extract leads to significantly increased activity of respiratory complex I and citrate synthase, as well as increased accumulation of various subunits of respiratory enzyme complexes. Moreover, upregulation of the stress-induced alternative oxidase as well as the NAD?-specific isocitrate dehydrogenase and mitochondrial ascorbate peroxidase was detected in the mitochondria of plants exposed to N. spumigena AV1 extract, while no difference in the carbonylation level of the mitochondrial proteins could be detected between the control and the exposed plants.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

35

Numer

03

Opis fizyczny

p.969-974,fig.,ref.

Twórcy

autor
  • Molecular Plant Biology, Department of Biochemistry and Food Chemistry, University of Turku, Biocity A 6th floor, Tykisto¨katu 6, Turku 20520, Finland
autor
  • Institute of Clinical Medicine, Neurology, University of Oulu, Oulu, Finland
  • Clinical Research Center, Oulu University Hospital, Oulu, Finland
autor
  • Molecular Plant Biology, Department of Biochemistry and Food Chemistry, University of Turku, Biocity A 6th floor, Tykisto¨katu 6, Turku 20520, Finland
autor
  • Institute of Clinical Medicine, Neurology, University of Oulu, Oulu, Finland
  • Clinical Research Center, Oulu University Hospital, Oulu, Finland
autor
  • Institute of Clinical Medicine, Neurology, University of Oulu, Oulu, Finland
  • Clinical Research Center, Oulu University Hospital, Oulu, Finland
  • Institute of Clinical Medicine, Pediatrics, University of Oulu, Oulu, Finland
autor
  • Institute of Clinical Medicine, Neurology, University of Oulu, Oulu, Finland
  • Clinical Research Center, Oulu University Hospital, Oulu, Finland
autor
  • Department of Food and Environmental Sciences, University of Helsinki, Helsinki, Finland
autor
  • Molecular Plant Biology, Department of Biochemistry and Food Chemistry, University of Turku, Biocity A 6th floor, Tykisto¨katu 6, Turku 20520, Finland
autor
  • Molecular Plant Biology, Department of Biochemistry and Food Chemistry, University of Turku, Biocity A 6th floor, Tykisto¨katu 6, Turku 20520, Finland

Bibliografia

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  • Carmichael WW, Eschedor JT, Patterson GM, Moore RE (1988) Toxicity and partial structure of a hepatotoxic peptide produced by the cyanobacterium Nodularia spumigena Mertens emend. L575 from New Zealand. Appl Environ Microbiol 54:2257–2263
  • Chrzanowska-Lightowlers ZM, Turnbull DM, Lightowlers RN (1993) A microtiter plate assay for cytochrome c oxidase in permeabilized whole cells. Anal Biochem 214:45–49
  • Codd GA, Metcalf JS, Beattie KA (1999) Retention of Microcystis aeruginosa and microcystin by salad lettuce (Lactuca sativa) after spray irrigation with water containing cyanobacteria. Toxicon 37:1181–1185
  • Davies WR, Siu WH, Jack RW, Wu RS, Lam PK, Nugegoda D (2005) Comparative effects of the blue green algae Nodularia spumigena and a lysed extract on detoxification and antioxidant enzymes in the green lipped mussel (Perna viridis). Mar Pollut Bull 51:1026–1033
  • Day DA, Neuburger M, Douce R (1985) Biochemical characterization of chlorophyll-free mitochondria from pea leaves. Aust J Plant Physiol 12:219–228
  • Fewer DP, Jokela J, Rouhiainen L, Wahlsten M, Koskenniemi K, Stal LJ, Sivonen K (2009) The non-ribosomal assembly and frequent occurrence of the protease inhibitors spumigins in the bloomforming cyanobacterium Nodularia spumigena. Mol Microbiol 73:924–937
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  • Kankaanpää H, Leiniö S, Olin M, Sjövall O, Meriluoto J, Lehtonen KK (2007) Accumulation and depuration of cyanobacterial toxin nodularin and biomarker responses in the mussel Mytilus edulis. Chemosphere 68:1210–1217
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  • Lehtimäki J, Moisander P, Sivonen K, Kononen K (1997) Growth, nitrogen fixation, and nodularin production by two Baltic Sea cyanobacteria. Appl Environ Microbiol 63:1647–1656
  • Lehtimäki N, Shunmugam S, Jokela J, Wahlsten M, Carmel D, Keränen M, Sivonen K, Aro EM, Allahverdiyeva Y, Mulo P (2011) Nodularin uptake and induction of oxidative stress in spinach (Spinachia oleracea). J Plant Physiol 168:594–600
  • Liu S (1997) Generating, partitioning, targeting and functioning of superoxide in mitochondria. Biosci Rep 17:259–272
  • Møller IM (2001) Plant mitochondria and oxidative stress: electron transport, NADPH turnover, and metabolism of reactive oxygen species. Ann Rev Plant Physiol Plant Mol Biol 52:561–591
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  • Persson KJ, Legrand C, Olsson T (2009) Detection of nodularin in European flounder (Platichthys flesus) in the west coast of Sweden: evidence of nodularin mediated oxidative stress. Harmful Algae 8:832–838
  • Pflugmacher S, Olin M, Kankaanpää H (2007) Nodularin induces oxidative stress in the Baltic Sea brown alga Fucus vesiculosus (Phaeophyceae). Mar Environ Res 64:149–159
  • Pflugmacher S, Olin M, Kankaanpää H (2010) Oxidative stress response in the red alga Furcellaria lumbricalis (Huds.) Lamour. due to exposure and uptake of the cyanobacterial toxin nodularin from Nodularia spumigena. Harmful Algae 10:49–55
  • Rasmusson AG, Geisler DA, Møller IM (2008) The multiplicity of dehydrogenases in the electron transport chain of plant mitochondria. Mitochondrion 8:47–60
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  • Sagardoy R, Morales F, Rella´n-A´ lvarez R, Abadı´a A, Abadı´a J, Lo´pez-Milla´n AF (2011) Carboxylate metabolism in sugar beet plants grown with excess Zn. J Plant Physiol 168:730–733
  • Tiwari BS, Belenghi B, Levine A (2002) Oxidative stress increased respiration and generation of reactive oxygen species, resulting in ATP depletion, opening of mitochondrial permeability transition, and programmed cell death. Plant Physiol 128:1271–1281
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Bibliografia

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