Molecular screening of gastric Helicobacter pullorum recovered from different avian species in Egypt

• Autorzy: Elhelw R. , Elhariri M. , Salem H.M. , Khalefa H.S. , Hamza D.A. , Ahmed Z.S.
• Języki publikacji: EN

Abstrakty

EN

Helicobacter pullorum (H. pullorum) is a bacterium that colonizes the intestines of poultry and causes gastroenteritis. Because these species are known as human and/or animal pathogens, identification of H. pullorum is becoming increasingly necessary. The bacterium has been linked to colitis and hepatitis in humans after being transmitted by infected meat consumption. Misdiagnosis of other enteric zoonotic pathogens such as Campylobacter and other Helicobacter species makes the diagnosis of H. pullorum extremely difficult. This study focused on the molecular detection of H. pullorum from the stomach (proventriculus and gizzard) of different avian species as new target organs for detection and transmission between avian species. Proventriculus and gizzards were obtained from 40 freshly dead chickens and resident wild birds (n=40). Diarrhea was found in the farms that were surveyed. DNA was extracted from all collected samples to conduct PCR amplification. The samples were screened for Helicobacter genus-specific 16s using C97 and C05 primers. To confirm the existence of H. pullorum, the positive samples were sequenced. H. pullorum was recorded in two out of 40 chicken samples. In addition, H. pullorum was recorded in one out of 40 resident wild birds. The 16S rRNA gene sequence for Helicobacter genus-specific in poultry and wild birds showed a 100% homology. In conclusion, broiler chickens and resident wild birds are possible reservoirs for H. pullorum, according to this report, and possibly act as a source of infection for humans via the food supply.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2022
• Tom: 25
• Numer: 3
• Opis fizyczny: p.369-374,fig.,ref.

Twórcy

autor

Elhelw R.

• Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Egypt

autor

Elhariri M.

• Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Egypt

autor

Salem H.M.

• Department of Poultry Diseases, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt

autor

Khalefa H.S.

• Department of Veterinary Hygiene and Management, Faculty of Veterinary Medicine, Cairo University, Egypt

autor

Hamza D.A.

• Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, P.O. Box 12211, Giza, Egypt

autor

Ahmed Z.S.

• Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, P.O. Box 12211, Giza, Egypt

Identyfikatory

DOI

10.24425/pjvs.2022.142019