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Czasopismo

2012 | 71 | 2 |

Tytuł artykułu

The use of computer-assisted image analysis in measuring the histological structure of the human median nerve

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Background and aim: The aim of this study was to assess the histological structure of the median nerve and its motor branch (number and arrangement of nerve bundles) and the cross-sectional area (CSA) of the median nerve (on the level of the carpal tunnel). Material and methods: This study has been conducted using median nerves dissected from cadavers stored in a 10% solution of formaldehyde at the Department of Anatomy of the Jagiellonian University Medical College and cadavers from the Department of Forensic Medicine of the Jagiellonian University Medical College. After dissection the median nerves were stained with haematoxylin and eosin and histological slides were prepared. These were later photographed (16¥ magnification) and analysed using ImageJ software. The research protocol was approved by the Jagiellonian University Ethics Committee (registry KBET/209/B/2002). Results: The studied group comprised 8 women and 22 men (age between 23–92 years), yielding a total of 60 median nerves (30 right vs. 30 left). In 4 (6.67%) cases an accessory motor branch was found. The mean CSA of the median nerve was 0.19 cm2. The median nerves from the right hand had a statistically larger CSA (p = 0.017). The number of nerve bundles in the median nerve varied between 13 to 38 and in the motor branch of the median nerve between 4 to 14. Conclusions: The nerve bundles of the median nerve, at the level of the carpal tunnel, display no particular type of arrangement. ImageJ software proved useful in the assessment of the histological structure of the human median nerve and its motor branch. (Folia Morphol 2012; 71, 2: 82–85)

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Wydawca

-

Czasopismo

Rocznik

Tom

71

Numer

2

Opis fizyczny

p.82-85,fig.,ref.

Twórcy

autor
  • Department of Anatomy, Jagiellonian University Medical College, ul.Kopernika 12, 31–034 Krakow, Poland
  • Department of Anatomy, Jagiellonian University Medical College, ul.Kopernika 12, 31–034 Krakow, Poland
autor
  • Department of Histology, Jagiellonian University Medical College, Krakow, Poland
autor
  • Department of Anatomy, Jagiellonian University Medical College, ul.Kopernika 12, 31–034 Krakow, Poland
autor
  • Institute of Physiotherapy, Jagiellonian University Medical College, Krakow, Poland

Bibliografia

  • 1. Casanova-Molla J, Morales M, Sola-Valls N, Bosch A, Calvo M, Grau-Junyent JM, Valls-Sole J (2011) Axonal fluorescence quantitation provides a new approach to assess cutaneous innervation. J Neurosci Methods, 200: 190–198.
  • 2. Clarebrough EE, Challacombe BJ, Briggs C, Namdarian B, Waston R, Murphy DG, Costello AJ (2011) Cadaveric analysis of periprostatic nerve distribution: an anatomical basis for high anterior release during radical prostatectomy? J Urol, 185: 1519–1525.
  • 3. Demircay E, Civelek E, Cansever T, Kabatas S, Yilmaz C (2011) Anatomic variations of the median nerve in the carpal tunnel: a brief review of the literature. Turk Neurosurg, 21: 388–396.
  • 4. Endo H, Kawahara K (2011) Gender differences in hand stability of normal young people assessed at low force levels. Ergonomics, 54: 273–281.
  • 5. Ganzer R, Blana A, Stolzenburg JU, Rabenalt R, Fritsche HM, Wieland WF, Denzinger S (2009) Nerve quantification and computerized planimetry to evaluate periprostatic nerve distribution-does size matter? Urology, 74: 398–403.
  • 6. Mizia E, Klimek-Piotrowska W, Walocha J, Rutowski R, Wojtala R (2011) The median nerve in the carpal tunnel. Folia Morphol, 70: 41–46.
  • 7. Sharma S, Ray B, Bhardwaj D, Dwivedi AK, Roy TS (2009) Age changes in the human oculomotor nerve — a stereological study. Ann Anat, 191: 260–266.
  • 8. Yao L, Gai N (2009) Median nerve cross-sectional area and MRI diffusion characteristics: normative values at the carpal tunnel. Skeletal Radiol, 38: 355–361.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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