Micropropagation of Eryngium campestre L. via shoot culture provides valuable uniform plant material with enhanced content of phenolic acids and antimicrobial activity

• Autorzy: Kikowska M. , Thiem B. , Sliwinska E. , Rewers M. , Kowalczyk M. , Stochmal A. , Dlugaszewska J.
• Języki publikacji: EN

Abstrakty

EN

An efficient micropropagation protocol for production of genetically uniform clones of Eryngium campestre L. was developed. To determine the effect of nutritional and hormonal factors on shoot and root development and bioactive compounds production, three variants of media differing in the content of macro- and micronutrients, as well as plant growth regulators of various types and concentrations were tested. The highest regeneration (100%), with over 13 shoots per explant, was induced on Murashige and Skoog (MS) medium with 1.0 mg lˉ¹ benzyladenine (BA) and 0.1 mg lˉ¹ indole-3-acetic acid (IAA). The in vitro derived shoots multiplied through axillary bud formation were rooted and transferred to an experimental plot with 78% frequency of survival. Flow cytometry showed no variation in nuclear DNA between the seedlings and micropropagated plants. Preliminary thin layer chromatography (TLC) analysis indicated that phenolic acids, saponins, flavonoids and acetylenes were present in plant biomass. Ultra high performance liquid chromatography (UHPLC) analysis revealed that shoots and roots from in vitro derived plants and root cultures maintained the ability to produce rosmarinic acid (RA), rosmarinic acid hexoside (RA-HEX) and chlorogenic acid (CGA). The highest phenolic acid content was detected in roots of in vitro regenerated plants. The extract from those roots expressed the highest inhibitory effect against bacteria Staphylococcus aureus, as well as dermatophytes Trichophyton mentagrophytes and T. rubrum.

Słowa kluczowe

EN

field eryngo; Eryngium campestre; shoot culture; plant material; phenolic acid content; chlorogenic acid; rosmarinic acid; antimicrobial activity; flow cytometry; micropropagation

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2016
• Tom: 58
• Numer: 1
• Opis fizyczny: p.43-56,fig.,ref.

Twórcy

autor

Kikowska M.

• Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, Sw. Marii Magdaleny 14, 61-861 Poznan, Poland

autor

Thiem B.

• Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, Sw. Marii Magdaleny 14, 61-861 Poznan, Poland

autor

Sliwinska E.

• Laboratory of Molecular Biology and Cytometry, Department of Plant Genetics, Physiology and Biotechnology, University of Science and Technology, al. Prof. S. Kaliskiego 7, 85-789 Bydgoszcz, Poland

autor

Rewers M.

• Laboratory of Molecular Biology and Cytometry, Department of Plant Genetics, Physiology and Biotechnology, University of Science and Technology, al. Prof. S. Kaliskiego 7, 85-789 Bydgoszcz, Poland

autor

Kowalczyk M.

• Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich 8, 24-100 Pulawy, Poland

autor

Stochmal A.

• Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich 8, 24-100 Pulawy, Poland

autor

Dlugaszewska J.

• Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medicinal Sciences, Swiecickiego 4, 60–781 Poznan, Poland

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Identyfikatory

DOI

10.1515/abcsb-2016-0009