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2015 | 18 | 3 |
Tytuł artykułu

Characteristic and susceptibility to enterocins of enterococci in pheasants possessing virulence factor genes

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
With an increasing number of pheasants as gamebirds being reared each year, these species are becoming a more prominent part of the workload of many veterinary practices. Only limited information can be found concerning the microflora of common pheasants. A significant part of the obligate microflora consists of lactic acid bacteria, including enterococci. In this study, faeces were sampled from 60 pheasants aged 16-17 weeks. Enterococcal counts reached 5.48±1.9 (log10) CFU/g. Strains (17) were taxonomically classified to the genus Enterococcus using the Maldi-Tof identification system; they were allotted to the species E. hirae (58.8%), E. faecium (23.5%) and E. faecalis (17.7%) by highly probable species identification or by secure genus identification/probable species identication. Species allocation was also confirmed using conventional biochemical tests. Most strains formed β-hemolysis. Gelatinase active phenotype was found in three E. faecalis strains. Enterococci were β-glucuronidase negative, mostly trypsin negative with slight or moderate production of α-chymotrypsin. EH52b and EF42 strains possessed the highest potential for pathogenicity. Average value of lactic acid was 1.78±0.33 mmo/L. Most strains were tetracycline resistant (82.4%). Polyresistant E. faecalis strains with positive gelatinase phenotype and possessing virulence factor genes confirmed using PCR (gelE, efaAfs, ccf cob, cpd) were sensitive to enterocins (activity 1600-25 600 AU/mL).
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
18
Numer
3
Opis fizyczny
p.507-514,fig.,ref.
Twórcy
  • Institute of Animal Physiology, Slovak Academy of Sciences, Soltesovej 4-6, 040 01 Kosice, Slovakia
autor
  • Institute of Animal Physiology, Slovak Academy of Sciences, Soltesovej 4-6, 040 01 Kosice, Slovakia
  • Institute of Animal Physiology, Slovak Academy of Sciences, Soltesovej 4-6, 040 01 Kosice, Slovakia
Bibliografia
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Typ dokumentu
Bibliografia
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Identyfikator YADDA
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