Development of in vitro methods for regeneration of strawberry ‘Festival’ and ‘Rubygem’ varieties (Fragaria × annanasa Duch.)

• Autorzy: Sonmez D.A. , Kafkas E.

Warianty tytułu

PL

Rozwój metod regeneracji in vitro odmian truskawki 'Festival' i 'Rubygem'

• Języki publikacji: EN

Abstrakty

EN

The cultivated strawberry (Fragaria × ananassa Duch.), a member of the Rosaceae, is the most important soft fruit in the world. In vitro micropropagation is an important tool for clonal multiplication and is commonly applied for induction of somaclonal variation to create genetic variability. Optimized regeneration protocols and high efficiency are also important for gene transformation for improving new cultivars. For this purpose, in this study it was aimed to optimize a regeneration protocol for ‘Festival’ and ‘Rubygem’ strawberry varieties. Leaf disks and stipules from mature in vitro grown strawberry plants of ‘Festival’ and ‘Rubygem’ varieties (Fragaria × annanasa Duch.) were used as explant sources. Mc Cowns Woody plants including vitamins were used as a basal medium. Different concentrations of TDZ (Thidiazuron) (0, 1, 2, 3 and 4 mg l⁻¹) and IBA (Indole-3-butyric acid) (0, 0.2 and 0.4 mg l⁻¹) and the efficiency of 15 days dark treatment on the regeneration from leaf disks and stipules were also tested. Our results suggested that leaf stipules, a high concentration of TDZ, AgNO3 and a dark treatment in culture medium successfully resulted in the induction of regeneration.

PL

Truskawka uprawna (Fragaria × ananassa Duch.), należąca do rodziny Rosaceae, jest najważniejszym owocem miękkim na świecie. Mikrorozmnażanie in vitro jest ważnym narzędziem do rozmnażania przez klonowanie i jest powszechnie stosowane do wywoływania odmiany somaklonalnej dla wytworzenia zmienności genetycznej. Zoptymalizowane protokoły regeneracji oraz wysoka wydajność również są ważne dla transformacji genów w celu ulepszenia nowych odmian. Dlatego celem niniejszej pracy była optymalizacja protokołu regeneracji dla odmian truskawek ‘Festival’ i ‘Rubygem’. Blaszki liściowe oraz przylistki dojrzałych wyhodowanych in vitro roślin truskawek odmian ‘Festival’ i ‘Rubygem’ (Fragaria × annanasa Duch.) wykorzystano jako źródła eksplantów. Rośliny Mc Cowns Woody zawierające witaminy wykorzystano jako pożywkę zasadową. Przetestowano również różne stężenia TDZ (Tidiazuron) (0, 1, 2, 3 i 4 mg l⁻¹) i IBA (kwas indolowo-3-masłowy) (0, 0,2 i 0,4 mg l⁻¹) oraz wpływ 15-dniowego zaciemnienia na regenerację z blaszek liściowych i przylistków. Nasze wyniki sugerowały, że przylistki, wysokie stężenie TDZ, AgNO3 oraz zaciemnianie w pożywce hodowlanej pozytywnie wpłynęły na wywołanie regeneracji.

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2012
• Tom: 11
• Numer: 4
• Opis fizyczny: p.129-142,ref.

Twórcy

autor

Sonmez D.A.

• Yaltir A.S., Sarihuglar Mah., Adana, Turkey

autor

Kafkas E.

• Department of Horticulture and Biotechnology Research and Experimental Center, Faculty of Agriculture, University of Cukurova, 01330, Balcali, Adana, Turkey

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