PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2009 | 31 | 3 |

Tytuł artykułu

Biochemical responses to drought stress in mulberry (Morus alba L.): evaluation of proline, glycine betaine and abscisic acid accumulation in five cultivars

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Five popularly grown mulberry cultivars (K-2, MR-2, TR-10, BC2-59 and S-13) were subjected to drought stress by withholding irrigation, to obtain leaf water potentials (Ψw) ranging from-0.75,-1.50 and-2.25 MPa. Accumulation of proline, glycine betaine and abscisic acid (ABA) were quantified in control and water stressed mulberry leaves. The activities of enzymes involved in proline accumulation including glutamate dehydrogenase (EC1.4.1.2-4), pyrroline-5-carboxylate synthetase (EC 1.2.1.41), pyrroline-5-carboxylate reductase (EC1.5.1.2), ornithine transaminase (EC 2.6.1.13) were significantly enhanced in the leaves of all the cultivars with decreasing leaf water potentials, while the activities of proline dehydrogenase (EC 1.5.1.2) were reduced with progressive increase in water stress. Accumulation of proline, glycine betaine and abscisic acid was relatively higher in S-13 and BC2-59 compared to K-2, MR-2 and TR-10 under water deficit conditions. Our results demonstrate that S-13 and BC2-59 have superior osmoprotectant mechanisms under water-limited growth regimes.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

31

Numer

3

Opis fizyczny

p.437-443,fig.,ref.

Twórcy

  • Department of Plant Biology and Biotechnology, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India
  • Department of Plant Biology and Biotechnology, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India
autor
  • Department of Plant Biology and Biotechnology, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India

Bibliografia

  • Ashraf M, Foolad MR (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot 59:206–216. doi:10.1016/j.envexpbot.2005.12.006
  • Balarathinasabapathi (2000) Metabolic engineering for stress tolerance: installing osmoprotectant synthesis pathways. Ann Bot (Lond) 86:709–716
  • Bradford MM (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of proteindye-binding. Anal Biochem 72:248–254. doi:10.1016/0003-2697(76)90527-3
  • Chen THH, Murata N (2002) Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes. Curr Opin Plant Biol 5:250–257. doi:10.1016/S1369-5266(02)00255-8
  • Chopra KR, Selote DS (2007) Acclimation to drought stress generates oxidative stress tolerance in drought-resistant than susceptible wheat cultivar under field conditions. Environ Exp Bot 60:276–283. doi:10.1016/j.envexpbot.2006.11.004
  • Cominelli E, Galbiati M, Vavasseur A, Conti L, Sala T, Vuylsteke M, Leonhardt N, Dellaporta SL, Tonelli C (2005) A guard-cellspecific MYB transcription factor regulates stomatal movements and plant drought tolerance. Curr Biol 15:1196–1200. doi:10.1016/j.cub.2005.05.048
  • Delauney AJ, Verma DPS (1993) Proline biosynthesis and osmoregulation in plants. Plant J 4:215–223. doi:10.1046/j.1365-313X. 1993.04020215.x
  • Deuschle K, Funk D, Hellmann H, Daschner K, Binder S, FrommerWB (2001) A nuclear gene encoding mitochondrial Δ1-Pyrroline-5-carboxylate-dehydrogenase and its potential role in protection from proline toxicity. Plant J 27:345–355. doi:10.1046/j.1365-313X.2001.01101.x
  • Finkelstein RR, Gampala SSL, Rock CD (2002) Abscisic acid signalling in seeds and seedlings. Plant Cell 14:S15–S45
  • Hare PD, Cress WA, Van Staden J (1998) Dissecting the roles of osmolyte accumulation during stress. Plant Cell Environ 21:535–553. doi:10.1046/j.1365-3040.1998.00309.x
  • Hellmann H, Funck D, Rentsch D, Frommer WB (2000) Hypersensitivity of an Arabidopsis sugar signalling mutant toward exogenous proline application. Plant Physiol 123:779–790. doi:10.1104/pp.123.2.779
  • Hoagland DR, Arnon DI (1950) The water culture method for growing plants without soil. Cal Agric Exp Stn 397:1–32
  • Hong ZL, Lakkineni K, Zhang ZM, Verma DPS (2000) Removal of feedback inhibition of D1-pyrroline-5-carboxylate synthetase results in increased proline accumulation and protection of plants from osmotic stress. Plant Physiol 122:1129–1136. doi:10.1104/pp.122.4.1129
  • Hua X-J, Cotte VDB, Montago VM, Verbruggen N (1997) Developmental regulation of pyrroline-5-carboxylate reductase gene expression in Arabidopsis. Plant Physiol 114:1215–1224. doi:10.1104/pp.114.4.1215
  • Kavi Kishore PB, Sangam S, Amrutha RN, Laxmi PS, Naidu KR, Rao KRSS, Rao S, Reddy KJ, Theriappan P, Sreenivasulu N (2005) Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance. Curr Sci 88:424–438
  • Konomori T, Konishi S, Takahashi E (1972) Inducible formation of glutamate dehydrogenase in rice plant roots by the addition of ammonia to the media. Physiol Plant 26:1–6. doi:10.1111/j. 1399-3054.1972.tb03536.x
  • Lawlor DW, Cornic G (2002) Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. Plant Cell Environ 25:275–294. doi:10.1046/j.0016-8025.2001.00814.x
  • Mazelis M, Fowden L (1969) Conversion of ornithine to proline by enzymes from germinating peanut cotyledens. Phytochemistry 8:801–809. doi:10.1016/S0031-9422(00)85863-X
  • Miler PM, Stewart CR (1976) Pyrroline-5-carboxylic acid reductase from soybean leaves. Phytochemistry 15:1855–1857. doi:10.1016/S0031-9422(00)88830-5
  • Papageorgiou GC, Morata N (1995) The usually strong stabilizing effects of glycine betaine on the structure and function in the oxygen evolving photosystem-II complex. Photosynth Res 44:243–252. doi:10.1007/BF00048597
  • Raymond MJ, Smirnoff N (2002) Proline metabolism and transport in maize seedlings at low water potential. Ann Bot (Lond) 89:813–823. doi:10.1093/aob/mcf082
  • Reddy Ramachandra A, Chaitanya KV, Jutur PP, Sumithra K (2004) Differential antioxidative responses to water stress among five mulberry (Morus alba L.) cultivars. Environ Exp Bot 52:33–42. doi:10.1016/j.envexpbot.2004.01.002
  • Reno AB, Splittstoesser WE (1975) Proline dehydrogenase and pyrroline-5-carboxylate reductase from pumpkin cotyledons. Phytochemistry 14:657–661. doi:10.1016/0031-9422(75) 83010-X
  • Rios MG, Tomom CH, Fugita P, Rosa CL, Rosy RD, Clithero JM, Bressan RA, Csanka L (1997) Cloning of polycistronic cDNA from tomato encoding c-glutamyl phosphate reductase. Proc Natl Acad Sci USA 94:8249–8254. doi:10.1073/pnas.94.15.8249
  • Savoure A, Jaoua S, Hua X-J, Ardiles W, Van Montagu M, Verbruggen N (1995) Isolation, characterization, and chromosomal location of a gene encoding the D1-pyrroline-5 carboxylate synthetase in Arabidopsis thaliana. FEBS Lett 372:13–19. doi:10.1016/0014-5793(95)00935-3
  • Shevyakova NI (1984) Metabolism and the physiological role of proline in plants under conditions of plants and water stress. Sov Plant Physiol 31:597–608
  • Shinozaki K, Yamaguchi-Shinozaki K (1999) Molecular responses to drought stress. In: Yamaguchi-Shinaozaki (ed) Molecular responses to cold, drought, heat and salt stress in higher plants. R. G. Landes Company, Austin, pp 11–28
  • Su J, Hirji R, Zhang L, He C, Selvaraj G, Wu R (2006) Evaluation of the stress-inducible production of choline oxidase in transgenic rice as a strategy for producing the stress-protectant glycine betaine. J Exp Bot 57:1129–1135. doi:10.1093/ jxb/erj133
  • Szoke A, Miao GH, Hong Z, Verma DPS (1992) Subcellular localisation of D1-pyrroline-5-carboxylate reductase in root, nodule and leaf of soybean. Plant Physiol 99:1642–1649
  • Trinchant J-C, Boscari A, Spennato G, Sype VDG, Rudulier DL (2004) Proline betaine accumulation and metabolism in Alfalfa plants under sodium chloride stress. Exploring its compartmentalization in nodules. Plant Physiol 135:1583–1594. doi:10.1104/ pp.103.037556
  • Verslues PE, Bray EA (2006) Role of abscisic acid (ABA) and Arabidopsis thaliana ABA-insensitive loci in low water potential-induced ABA and proline accumulation. J Exp Bot 57:201–212. doi:10.1093/jxb/erj026
  • Verslues PE, Sharp RE (1999) Proline accumulation in maize (Zea mays L.) primary roots at low water potentials. II. Metabolic source of increased proline deposition in the elongation zone. Plant Physiol 119:1349–1360. doi:10.1104/pp.119.4.1349
  • Wood AJ, Saneoka H, Rhodes D, Joly RJ, Goldsbrough PB (1996) Betaine aldehyde dehydrogenase in sorghum molecular cloning and expression of two related genes. Plant Physiol 110:1301–1308. doi:10.1104/pp.110.4.1301
  • Xiang Y, Huang Y, Xiong L (2007) Characterization of stressresponsive CIPK genes in rice for stress tolerance improvement. Plant Physiol 144:1416–1428. doi:10.1104/pp.107.101295
  • Yamada M, Morishita H, Urano K, Shiozaki N, Yamaguchi-Shinozaki K, Shinozaki K, Yoshiba Y (2005) Effects of free proline accumulation in petunias under drought stress. J Exp Bot 56:1975–1981. doi:10.1093/jxb/eri195
  • Yoshiba Y, Kiyosue T, Katagiri T, Ueda H, Mizoguchi T, Yamaguchi-Shinozaki K, Wada K, Harada Y, Shinozaki K (1995) Correlation between the induction of a gene for D1- pyrroline-5-carboxylate synthetase and the accumulation of proline in Arabidopsis thaliana under osmotic stress. Plant J 7:751–760. doi:10.1046/j.1365-313X.1995.07050751.x
  • Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:247–273. doi:10.1146/annurev.arplant. 53.091401.143329

Uwagi

Rekord w opracowaniu

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-7f30dce2-a1ef-4961-98dc-f8939af135aa
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.