Identification of phosphate-starvation-inducible gene BnIPS1 in Brassica napus

• Autorzy: Yang G , Du H. , Xu H. , Ding G. , Xu F.
• Języki publikacji: EN

Abstrakty

EN

TPSI1/Mt4 family genes are a class of genes induced dramatically by Pi starvation, and are involved in Pi allocation in plants. Its members have been isolated from many plant species, but none has been reported in Brassica napus. Here, we isolated two novel members of TPSI1/Mt4 family from B. napus, which were referred to as BnIPS1;1 and BnIPS1;2. The two genes are 592 and 557 bp long, respectively, and share 91 % sequence identity. They are all intronless, and contain numerous short open reading frames and a conserved 22-nucleotide sequence partially complementary to miR399. Expression analysis revealed that the two genes were induced strongly by Pi starvation and weakly by osmotic stress and salinity. The induction of the two genes is rapid and durative in the absence of Pi and is repressible upon Pi resupply. Furthermore, BnIPS1;1 promoter (-1,459/?42 relative to transcription start site) was isolated and fused to reporter gene GUS. The PBnIPS1;1:GUS construct was introduced into Arabidopsis, and intensive GUS staining was observed in Pi-starved plants. These results further our understanding of TPSI1/Mt4 family genes, and demonstrate that BnIPS1;1 and BnIPS1;2 can be used as tool for investigating Pi-starvation signaling in B. napus and BnIPS1; 1 promoter can be used as inducible promoter for cultivating P-efficient crops.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 07
• Opis fizyczny: p.2085-2094,fig.,ref.

Twórcy

autor

Yang G

• National Key Laboratory of Crop Genetic Improvement, Microelement Research Center, Huazhong Agricultural University, Wuhan 430070, China

autor

Du H.

• National Key Laboratory of Crop Genetic Improvement, Microelement Research Center, Huazhong Agricultural University, Wuhan 430070, China

autor

Xu H.

• National Key Laboratory of Crop Genetic Improvement, Microelement Research Center, Huazhong Agricultural University, Wuhan 430070, China

autor

Ding G.

• National Key Laboratory of Crop Genetic Improvement, Microelement Research Center, Huazhong Agricultural University, Wuhan 430070, China

autor

Xu F.

• National Key Laboratory of Crop Genetic Improvement, Microelement Research Center, Huazhong Agricultural University, Wuhan 430070, China

Bibliografia

• Aung K, Lin SI, Wu CC, Huang YT, Su CL, Chiou TJ (2006) pho2, a phosphate overaccumulator, is caused by a nonsense mutation in a microRNA399 target gene. Plant Physiol 141:1000–1011
• Bari R, Pant BD, Stitt M, Scheible WR (2006) PHO2, microRNA399, and PHR1 define a phosphate-signaling pathway in plants. Plant Physiol 141:988–999
• Buhtz A, Springer F, Chappell L, Baulcombe DC, Kehr J (2008) Identification and characterization of small RNAs from the phloem of Brassica napus. Plant J 53:739–749
• Burleigh SH, Harrison MJ (1997) A novel gene whose expression in Medicago truncatula roots is suppressed in response to colonization by vesicular–arbuscular mycorrhizal (VAM) fungi and to phosphate nutrition. Plant Mol Biol 34:199–208
• Burleigh SH, Harrison MJ (1998) Characterization of the Mt4 gene from Medicago truncatula. Gene 216:47–53
• Burleigh SH, Harrison MJ (1999) The down-regulation of Mt4-like genes by phosphate fertilization occurs systemically and involves phosphate translocation to the shoots. Plant Physiol 119:241–248
• Bustos R, Castrillo G, Linhares F, Puga MI, Rubio V, Pérez-Pérez J, Solano R, Leyva A, Paz-Ares J (2010) A central regulatory system largely controls transcriptional activation and repression responses to phosphate starvation in Arabidopsis. PLoS Genet 6:e1001102
• Chiou TJ, Aung K, Lin SI, Wu CC, Chiang SF, Su CL (2006) Regulation of phosphate homeostasis by microRNA in Arabidopsis. Plant Cell 18:412–421
• Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735–743
• Duan K, Yi K, Dang L, Huang H, Wu W, Wu P (2008) Characterization of a sub-family of Arabidopsis genes with the SPX domain reveals their diverse functions in plant tolerance to phosphorus starvation. Plant J 54:965–975
• Fang Z, Shao C, Meng Y, Wu P, Chen M (2009) Phosphate signaling in Arabidopsis and Oryza sativa. Plant Sci 176:170–180
• Franco-Zorrilla JM, Valli A, Todesco M, Mateos I, Puga MI, Rubio-Somoza I, Leyva A, Weigel D, Garcia JA, Paz-Ares J (2007) Target mimicry provides a new mechanism for regulation of microRNA activity. Nat Genet 39:1033–1037
• Higo K, Ugawa Y, Iwamoto M, Korenaga T (1999) Plant cis-acting regulatory DNA elements (PLACE) database: 1999. Nucleic Acids Res 27:297–300
• Hou XL, Wu P, Jiao FC, Jia QJ, Chen HM, Yu J, Song XW, Yi KK (2005) Regulation of the expression of OsIPS1 and OsIPS2 in rice via systemic and local Pi signalling and hormones. Plant Cell Environ 28:353–364
• Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5:387–405
• Kasuga M, Liu Q, Miura S, Yamaguchi-Shinozaki K, Shinozaki K (1999) Improving plant drought, salt and freezing tolerance by gene transfer of a single stress-inducible transcriptional factor. Nat Biotechnol 17:287–291
• Lin SI, Chiang SF, Lin WY, Chen JW, Tseng CY, Wu PC, Chiou TJ (2008) Regulatory network of microRNA399 and PHO2 by systemic signaling. Plant Physiol 147:732–746
• Liu C, Muchhal US, Raghothama KG (1997) Differential expression of TPS11, a phosphate starvation-induced gene in tomato. Plant Mol Biol 33:867–874
• Liu F, Wang Z, Ren H, Shen C, Li Y, Ling HQ, Wu C, Lian X, Wu P (2010) OsSPX1 suppresses the function of OsPHR2 in the regulation of expression of OsPT2 and phosphate homeostasis in shoots or rice. Plant J 62:508–517
• López-Bucio J, de la Vega OM, Guevara-Garcıa A, Herrera-Estrella L (2000) Enhanced phosphorus uptake in transgenic tobacco plants that overproduce citrate. Nat Biotechnol 18:450–453
• Lü J, Gao X, Dong Z, Yi J, An L (2012) Improved phosphorus acquisition by tobacco through transgenic expression of mitochondrial malate dehydrogenase from Penicillium oxalicum. Plant Cell Rep 31:49–56
• Martın AC, Del Pozo JC, Iglesias J, Rubio V, Solano R, De La Pena A, Leyva A, Paz-Ares J (2000) Influence of cytokinins on the expression of phosphate starvation responsive genes in Arabidopsis. Plant J 24:559–567
• Mukatira UT, Liu C, Varadarajan DK, Raghothama KG (2001) Negative regulation of phosphate starvation-induced genes. Plant Physiol 127:1854–1862
• Nagaharu U (1935) Genome analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Jpn J Bot 7:389–452
• Nilsson L,Müller R, Nielsen TH(2007) Increased expression of theMYBrelated transcription factor, PHR1, leads to enhanced phosphate uptake in Arabidopsis thaliana. Plant Cell Environ 30:1499–1512
• Plaxton WC, Tran HT (2011) Metabolic adaptations of phosphatestarved plants. Plant Physiol 156:1006–1015
• Raghothama KG (1999) Phosphate acquisition. Annu Rev Plant Physiol Plant Mol Biol 50:665–693
• Rubio V, Linhares F, Solano R, Martin AC, Iglesias J, Leyva A, Paz-Ares J (2001) A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae. Genes Dev 15:2122–2133
• Schünmann PHD, Richardson AE, Vickers CE, Delhaize E (2004) Promoter analysis of the barley Pht1;1 phosphate transporter gene identifies regions controlling root expression and responsiveness to phosphate deprivation. Plant Physiol 136:4205–4214
• Shin H, Shin HS, Dewbre GR, Harrison MJ (2004) Phosphate transport in Arabidopsis: pht1;1 and Pht1;4 play a major role in phosphate acquisition from both low- and high-phosphate environments. Plant J 39:629–642
• Shin H, Shin HS, Chen R, Harrison MJ (2006) Loss of At4 function impacts phosphate distribution between the roots and the shoots during phosphate starvation. Plant J 45:712–726
• Ticconi CA, Abel S (2004) Short on phosphate: plant surveillance and countermeasures. Trends Plant Sci 9:548–555
• Vance CP, Uhde-Stone C, Allan DL (2003) Phosphorus acquisition and use: critical adaptations by plants for securing a nonrenewable resource. New Phytol 157:423–447
• Venkatachalam P, Jain A, Sahi S, Raghothama K (2009) Molecular cloning and characterization of phosphate (Pi) responsive genes in Gulf ryegrass (Lolium multiflorum L.): a Pi hyperaccumulator. Plant Mol Biol 69:1–21
• Vincent R, Fraisier V, Chaillou S, Limani AM, Deleens E, Phillipson B, Douat C, Boutin JP, Hirel B (1997) Overexpression of a soybean gene encoding cytosolic glutamine synthetase in shoots of transgenic Lotus corniculatus L. plants triggers changes in ammonium assimilation and plant development. Planta 201: 424–433
• Wasaki J, Yonetani R, Shinano T, Kai M, Osaki M (2003) Expression of the OsPI1 gene, cloned from rice using cDNA microarray, rapidly responds to phosphorus status. New Phytol 158:239–248

Uwagi

rekord w opracowaniu