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2009 | 31 | 6 |
Tytuł artykułu

In vitro propagation of Psoralea corylifolia L. by somatic embryogenesis in cell suspension culture

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
An effective protocol was developed for in vitro propagation of Psoralea corylifolia via somatic embryogenesis in cell suspension culture. Embryogenic callus was obtained on Murashige and Skoog (MS) medium supplemented with 6 µM naphthaleneacetic acid (NAA) and 30 µM glutamine from transverse TCLs from 10-day-old hypocotyl explants with a 96.4% frequency. Embryogenic callus produced a higher number of somatic embryos (123.7 ± 1.24 per gram fresh weight callus) onMS medium containing 30 g l⁻¹ sucrose, 1 µM NAA, 4 µM benzyladenine (BA), 15 µM glutamine and 2 µM abscisic acid (ABA) after 4 weeks of culture. Somatic embryos successfully germinated (97.6%) on ½ MS medium containing 20 g l⁻¹ sucrose, 8 g l⁻¹ agar and supplemented with 2 µM BA, 1 µM ABA and 2 µM gibberellic acid (GA₃) within 2 weeks of culture. Somatic embryos developed into normal plants, which hardened with 100% efficiency in soil in a growth chamber. Plants were successfully transferred to greenhouse and subsequently established in the field. Plant survival percentage in the field differed with seasonal variations. Average psoralen content of 12.9 µg g⁻¹ DW was measured in different stages of somatic embryo development by high-performance liquid chromatography (HPLC). This protocol will be helpful for efficient propagation of elite clones on a mass scale, conservation efforts of this species and for secondary metabolites production studies.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
31
Numer
6
Opis fizyczny
p.1119-1127,fig.,ref.
Twórcy
autor
  • Plant Biotechnology Division, Department of Plant Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India
  • Department of Vegetables Research, The Volcani Center, The Agricultural Research Organization, 50250 Bet Dagan, Israel
autor
  • Plant Biotechnology Division, Department of Plant Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India
Bibliografia
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Bibliografia
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