Isolation and characterization of a G protein gamma subunit gene responsive to plant hormones and abiotic stresses in Brassica napus L

• Autorzy: Gao Y. , Li T. , Zhao Y. , Ren C. , Zhang Y. , Wang M.
• Języki publikacji: EN

Abstrakty

EN

G protein plays an important role in signaling pathways and is involved in various signal transduction systems in plant. A full-length cDNA encoding a putative G protein γ subunit (Gγ), designated as BnGG2, was isolated from Brassica napus L. BnGG2 is predicted to encode a precursor protein of 100 amino acid residues. The expression of BnGG2 in different B. napus tissues and developmental stages was analyzed by using real-time PCR. The results showed that BnGG2 expression level was high at the 7-day-old seedling stage, the bolting stage, and the fruiting stage. Moreover, BnGG2 was analyzed under four different plant hormones. All of the four tested hormones, abscisic acid (ABA), gibberellins acid 3 (GA₃), brassinosteroid (BR) and indole-3-acetic acid (IAA), triggered an induction of BnGG2 expression at different hormone concentrations. The expression of BnGG2 was significantly induced by the high concentrations of ABA, BR, and IAA. The expression of BnGG2 was also induced by low GA₃ concentrations and inhibited by high GA₃ concentrations. In addition, BnGG2 was responsive to different abiotic stresses. The BnGG2 was up-regulated in salt and drought stress and down-regulated in heat and cold stress. These results suggested that BnGG2 plays an important role in plant hormone signaling pathways and may be also involved in plant defense systems against environmental stresses in B. napus.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 2
• Opis fizyczny: p.391-399,fig.,ref.

Twórcy

autor

Gao Y.

• Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, Sichuan, People's Republic of China

autor

Li T.

• Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, Sichuan, People's Republic of China

autor

Zhao Y.

• Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, Sichuan, People's Republic of China

autor

Ren C.

• Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, Sichuan, People's Republic of China

autor

Zhang Y.

• Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, Sichuan, People's Republic of China

autor

Wang M.

• Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, Sichuan, People's Republic of China

Bibliografia

• Adjobo-Hermans MJW, Goedhart J, Gadella TWJ Jr (2006) Plant G protein heterotrimers require dual lipidation motifs of G alpha and G gamma and do not dissociate upon activation. J Cell Sci 119:5087–5097
• Asakura Y, Kurosaki F (2007) Cloning and expression of dcga gene encoding a subunit of GTP-Binding protein in carrot seedlings. Biol Pharm Bull 30:1800–1804
• Assmann SM (2002) Heterotrimeric and unconventional GTP binding proteins in plant cell signaling. Plant Cell 14:S355–S373
• Bishopp A, Mahonen AP, Helariutta Y (2006) Signs of change: hormone receptors that regulate plant development. Development 133:1857–1869
• Chen JG, Pandey S, Huang JR, Alonso JM, Ecker JR, Assmann SM, Jones AM (2004) GCR1 acts independently of heterotrimeric G protein in response to brassinosteroids and gibberellins in Arabidopsis seed germination. Plant Physiol 135:907–915
• Clapham DE, Neer EL (1993) New roles for G-protein βγ-dimers in transmembrane signalling. Nature 365:403–406
• Evanko DS, Thiyagarajan MM, Wedegaertner PB (2000) Interaction with Gβγ is required for membrane targeting and palmitoylation of Gα(s) and Gα(q). J Biol Chem 275:1327–1336
• Evanko DS, Thiyagarajan MM, Siderovski DP, Wedegaertner PB (2001) Gβγ isoforms selectively rescue plasma membrane localization and palmitoylation of mutant Gα(s) and Gα(q). J Biol Chem 276:23945–23953
• Gilman AG (1987) G proteins: transducers of receptor-generated signals. Annu Rev Biochem 56:615–649
• Gomez-Cadenas A, Zentella R, Walker-Simmons MK, Ho THD (2001) Gibberellin/abscisic acid antagonism in barley aleurone cells: site of action of the protein kinase PKABA1 in relation to gibberellin signaling molecules. Plant Cell 13:667–679
• Gubler F, Hughes T, Waterhouse P, Jacobsen J (2008) Regulation of dormancy in barley by blue light and after-ripening: effects on abscisic acid and gibberellin metabolism. Plant Physiol 147:886–896
• Gudermann T, Schoneberg T, Schultz G (1997) Functional and structural complexity of signal transduction via G-proteincoupled receptors. Annu Rev Neurosci 20:399–427
• Ishikawa A, Tsubouchi H, Iwasaki Y, Asahi T (1995) Molecular cloning and characterization of a cDNA for the alpha subunit of a G protein from rice. Plant Cell Physiol 36:353–359
• Ishikawa A, Isasaki Y, Asahi T (1996) Molecular cloning and characterization of a cDNA for the beta subunit of a G protein from rice. Plant Cell Physiol 37:223–228
• Jones AM (2002) G-protein-coupled signaling in Arabidopsis. Curr Opin Plant Biol 5:402–407
• Kaul S, Koo HL, Jenkins J, Rizzo M, Rooney T, Tallon LJ et al (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408:796–815
• Koornneef M, Bentsink L, Hilhorst H (2002) Seed dormancy and germination. Curr Opin Plant Biol 5:33–36
• Lim WK, Myung CS, Garrison JC, Neubig RR (2001) Receptor-G protein gamma specificity: gamma 11 shows unique potency for A(1) adenosine and 5-HT1A receptors. Biochemistry 40:10532–10541
• Ma H, Yanofsky MF, Meyerowitz EM (1990) Molecular cloning and characterization of GPA1, a G protein α subunit gene from Arabidopsis thaliana. Proc Natl Acad Sci USA 87:3821–3825
• Mason MG, Botella JR (2001) Isolation of a novel G-protein gammasubunit from Arabidopsis thaliana and its interaction with G beta. Biochim Biophys Acta 1520:147–153
• Misra S, Wu Y, Venkataraman G, Sopory SK, Tuteja N (2007) Heterotrimeric G-protein complex and G-protein-coupled receptor from a legume (Pisum sativum): role in salinity and heat stress and cross-talk with phospholipase C. Plant J 51:656–669
• Morris AJ, Malbon CC (1999) Physiological regulation of G proteinlinked signaling. Physiol Rev 79:1373–1430
• Neer EJ (1995) Heterotrimeric G proteins: organizers of transmembrane signals. Cell 80:249–257
• Obrdlik P, Neuhaus G, Merkle T (2000) Plant heterotrimeric G protein beta subunit is associated with membranes via protein interactions involving coiled-coil formation. FEBS Lett 476:208–212
• Offermanns S (2000) Mammalian G-protein function in vivo: new insights through altered gene expression. Rev Physiol Biochem Pharmacol 140:63–133
• Oki K, Inaba N, Kitagawa K, Fujioka S, Kitano H, Fujisawa Y, Kato H, Iwasaki Y (2009) Function of the alpha subunit of rice heterotrimeric G protein in brassinosteroid signaling. Plant Cell Physiol 50:161–172
• Perfus-Barbeoch L, Jones AM, Assmann SM (2004) Plant heterotrimeric G protein function: insights from Arabidopsis and rice mutants. Curr Opin Plant Biol 7:719–731
• Scott JK, Huang SF, Gangadhar BP, Samoriski GM, Clapp P, Gross RA, Taussig R, Smrcka AV (2001) Evidence that a protein–protein interaction ‘hot spot’ on heterotrimeric G protein bc subunits is used for recognition of a subclass of effectors. EMBO J 20:767–776
• Simon MI, Strathmann MP, Gautam N (1991) Diversity of G proteins in signal transduction. Science 252:802–808
• Spartz AK, Gray WM (2008) Plant hormone receptors: new perceptions. Genes Dev 22:2139–2148
• Temple BRS, Jones AM (2007) The plant heterotrimeric G-protein complex. Annu Rev Plant Biol 58:249–266
• Trusov Y, Rookes JE, Tilbrook K, Chakravorty D, Mason MG, Anderson D, Chen JG, Jones AM, Botella JR (2007) Heterotrimeric G protein c subunits provide functional selectivity in Gbc dimer signaling in Arabidopsis. Plant Cell 19:1235–1250
• Ueguchi-Tanaka M, Fujisawa Y, Kobayashi M, Ashikari M, Iwasaki Y, Kitano H, Matsuoka M (2000) Rice dwarf mutant d1, which is defective in the a subunit of the heterotrimeric G protein, affects gibberellin signal transduction. Proc Natl Acad Sci USA 97:11638–11643
• Ueguchi-Tanaka M, Nakajima M, Motoyuki A, Matsuoka M (2007) Gibberellin receptor and its role in gibberellin signaling in plants. Annu Rev Plant Biol 58:183–198
• Ullah H, Chen JG, Wang SC, Jones AM (2002) Role of a heterotrimeric G protein in regulation of Arabidopsis seed germination. Plant Physiol 129:897–907
• Ullah H, Chen JG, Temple B, Boyes DC, Alonso JM, Davis KR, Ecker JR, Jones AM (2003) The beta-subunit of the Arabidopsis G protein negatively regulates auxin-induced cell division and affects multiple developmental processes. Plant Cell 15:393–409
• Wang XF, Zhang DP (2008) Abscisic acid receptors: multiple signalperception sites. Ann Bot 101:311–317
• Wang HX, Weerasinghe RR, Perdue TD, Cakmakci NG, Taylor JP, Marzluff WF, Jones AM (2006) A golgi-localized hexose transporter is involved in heterotrimeric G protein-mediated early development in Arabidopsis. Mol Biol Cell 17:4257–4269
• Weiss CA, Garnaat CW, Mukai K, Hu Y, Ma H (1994) Isolation of cDNAs encoding guanine nucleotide-binding protein β-subunit homologues from maize (ZGB1) and Arabidopsis (AGB1). Proc Natl Acad Sci USA 91:9554–9558
• Yang GX, Matsuoka M, Iwasaki Y, Komatsu S (2003) A novel brassinolide-enhanced gene identified by cDNA microarray is involved in the growth of rice. Plant Mol Biol 52:843–854
• Zeng Q, Wang XJ, Running MP (2007) Dual lipid modification of Arabidopsis Gc-subunits is required for efficient plasma membrane targeting. Plant Physiol 143:1119–1131
• Zentella R, Zhang ZL, Park M, Thomas SG, Endo A, Murase K, Fleet CM, Jikumaru Y, Nambara E, Kamiya Y, Sun TP (2007) Global analysis of della direct targets in early gibberellin signaling in Arabidopsis. Plant Cell 19:3037–3057
• Zhao Y, Wang ML, Zhang YZ, Du LF, Pan T (2000) A chlorophyllreduced seedling mutant in oilseed rape, Brassica napus, for utilization in F1 hybrid production. Plant Breed 119:131–135

Uwagi

rekord w opracowaniu