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2017 | 14 |

Tytuł artykułu

Clinical manifestations of Plasmodium berghei ANKA infection in juvenile mice: a short case report

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Malaria is an important health and development challenge in Africa, Animal models most particularly mice, have long been employed to study malaria pathogenesis. This paper describes clinical manifestations due to Plasmodium berghei ANKA infection in juvenile mice as a model for understanding the complications of congenital malaria in neonates. Forty-five juvenile mice (5-7 days old) were acquired from University College Hospital, Ibadan and injected with 2×107 (0.2 ml) Plasmodium berghei ANKA parasitized red blood cells (PRBCs). The mice were then transported to the study site, kept in well-ventilated cages and fed daily with a balanced ration. Post-P. berghei infection, the mice were monitored daily for mortality. Clinical manifestations of experimental cerebral malaria (ECM) were assessed and confirmed if at least ruffled fur, hunching, wobbly gait, limb paralysis, convulsions, or coma was observed. Each sign was given a score of 1. Animals with scores ≥4 were considered to have severe ECM. In the experiment, 20 (44%) mice were lost due to natural cause (i.e. stress) at day 2. Between day 4 and 9, 25 (56%) of the study mice presented clinical signs of ECM. This included: ruffled fur – 25 (100%), hunching - 21 (84%), wobbly gait - 17 (68%), limb paralysis - 20 (80%), convulsions - 25 (100%). Survival rate and severity of ECM in the mice differs, 22 (88.0%) had severe ECM and 3 (12.0%) had mild ECM. This study has shown that parasite establishment and malaria complications can manifest as early as 4 days’ post P. berghei infection in 5-7 days old mice.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

14

Opis fizyczny

p.90-96,fig.,ref.

Twórcy

autor
  • Department of Pure and Applied Zoology, Federal University of Agriculture, Abeokuta, Nigeria
  • Department of Pure and Applied Zoology, Federal University of Agriculture, Abeokuta, Nigeria
autor
  • Department of Microbiology, Ogun State Polytechnic, Igbesa, Nigeria
autor
  • Department of Microbiology, Ogun State Polytechnic, Igbesa, Nigeria
autor
  • Department of Pure and Applied Zoology, Federal University of Agriculture, Abeokuta, Nigeria

Bibliografia

  • [1] Druilhe P., Hagan P., Rook G. A. W. (2002). The importance of models of infection in the studyof disease resistance. Trends Microbiol. 10(10), 38-46
  • [2] Basi, R., Fazalul Rahiman, S. S., Hasballah, K., Chong, W. C., Talib, H., Yam, M. F., Jabbarzare, M., Tie, T. H., Othman, F. Moklas, M. M., Abdullah, W. O. and Ahmad Z. (2012). Plasmodium berghei ANKA Infection in ICR Mice as a Model of Cerebral Malaria. Iranian J Parasitol. 7(4), 62-74
  • [3] Hunt N. H., Grau G. E. (2003). Cytokines: accelerators and brakes in the pathogenesis of cerebral malaria. Trends Immunol. 24(9), 491-499.
  • [4] Combes V., de Souza J. B., Renia L., Hunt N. H., Grau G. E. (2005). Cerebral malaria: which parasite? Which model? Drug Disc Today 2(2), 141-147
  • [5] Clark, I. A. and Rockett K. A. (1994). The cytokine theory of human cerebral malaria. Parasitol Today 10(10), 410-412
  • [6] Artavanis-Tsakonas K. and Riley E. M. (2002). Innate immune response to malaria: rapid induction of IFNγ from human NK cells by live Plasmodium falciparum-infected erythrocytes. J. Immunol. 169(6), 2956-2963
  • [7] Wu, J.J., Chen, G., Liu, J., Wang, T., Zheng, W and Co, Y. M. (2010). Natural regulatory T cells mediate the development of cerebral malaria by modifying the pro-inflammatory response. Parasitol Int. 59, 232–241
  • [8] Blandine Franke-Fayard, Holly Trueman, Jai Ramesar, Jacqui Mendoza, Maartenvan der Keur, Reiniervan der Linden, Robert E. Sinden, Andrew P. Waters, Chris J. Janse. A Plasmodium berghei reference line that constitutively expresses GFP at a high level throughout the complete life cycle. Molecular and Biochemical Parasitology, Volume 137, Issue 1, September 2004, Pages 23-33.
  • [9] R. Ferone, J. J. Burchall and G. H. Hitchings. Plasmodium berghei Dihydrofolate Reductase Isolation, Properties, and Inhibition by Antifolates. Molecular Pharmacology January 1969, 5 (1) 49-59
  • [10] Michael G. Paton, Guy C. Barker, Hiroyuki Matsuoka, Jai Ramesar, Chris J. Janse, Andy P. Waters, Robert E. Sinden. Structure and expression of a post-transcriptionally regulated malaria gene encoding a surface protein from the sexual stages of Plasmodium berghei. Molecular and Biochemical Parasitology, Volume 59, Issue 2, June 1993, Pages 263-275
  • [11] M C Seguin, F W Klotz, I Schneider, J P Weir, M Goodbary, M Slayter, J J Raney, J U Aniagolu, S J Green. Induction of nitric oxide synthase protects against malaria in mice exposed to irradiated Plasmodium berghei infected mosquitoes: involvement of interferon gamma and CD8+ T cells. The Journal of Experimental Medicine 1994, 1 July, 180 (1), 353
  • [12] Keishi Adachi, Hiroko Tsutsui, Shin-Ichiro Kashiwamura, Ekihiro Seki, Hiroki Nakano, Osamu Takeuchi, Kazuyoshi Takeda, Ko Okumura, Luc Van Kaer, Haruki Okamura, Shizuo Akira and Kenji Nakanishi. Plasmodium berghei Infection in Mice Induces Liver Injury by an IL-12- and Toll-Like Receptor/Myeloid Differentiation Factor 88-Dependent Mechanism. J Immunol, November 15, 2001, 167 (10), 5928-5934

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-7e04b704-b03b-40b8-9198-654ac564b440
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