Altered somatic mutation level and DNA repair gene expression in Arabidopsis thaliana exposed to ultraviolet C, salt, and cadmium stresses

• Autorzy: Kiselev K.V. , Ogneva Z.V. , Dubrovina A.S. , Suprun A/R. , Tyunin A.P.
• Języki publikacji: EN

Abstrakty

EN

It is known that somatic mutations arising during animal growth and ageing contribute to the development of neurodegenerative and other animal diseases. For plants, several studies showed that small-scale somatic DNA mutations accumulated during Arabidopsis life cycle. However, there is a lack of data on the influence of environmental stresses on somatic DNA mutagenesis in plants. In this study, we analyzed the effects of ultraviolet C (UV-C) irradiation, high soil salinity, and cadmium (CdI₃) stresses on the level of small-scale somatic DNA mutations in Arabidopsis thaliana. The number of DNA mutations was examined in the Actin2 3′UTR (Actin-U1), ITS1-5.8rRNA-ITS2 (ITS), and ribulose-1,5-biphosphate carboxylase/oxygenase (rbcL) DNA regions. We found that somatic mutation levels considerably increased in CdI₃-treated Arabidopsis plants, while the mutation levels declined in the UV-C- and NaCl-treated A. thaliana. Cadmium is a mutagen that is known to inhibit DNA repair processes. The detected stress-induced alterations in somatic DNA mutation levels were accompanied by markedly increased expression of base excision repair genes (AtARP, AtDME, AtDML2, AtDML3, AtMBD4, AtROS, AtUNG, and AtZDP), nucleotide excision repair genes (AtDDB1a, AtRad4, and AtRad23a), mismatch repair genes (AtMSH2, AtMSH3, and AtMSH7), and photoreactivation genes (AtUVR2, AtUVR3). Thus, the results demonstrated that UV-C, high soil salinity, and cadmium stresses influence both the level of DNA mutations and expression of DNA repair genes. Salt- and UV-induced activation of DNA repair genes could contribute to the stress-induced decrease in somatic mutation level.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2018
• Tom: 40
• Numer: 01
• Opis fizyczny: Article 21 [10p.], fig.,ref.

Twórcy

autor

Kiselev K.V.

• Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, Vladivostok 690022, Russia
• Department of Biochemistry, Biotechnology, and Microbiology, Far Eastern Federal University, Vladivostok 690090, Russia

autor

Ogneva Z.V.

• Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, Vladivostok 690022, Russia
• Department of Biochemistry, Biotechnology, and Microbiology, Far Eastern Federal University, Vladivostok 690090, Russia

autor

Dubrovina A.S.

• Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, Vladivostok 690022, Russia

autor

Suprun A/R.

• Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, Vladivostok 690022, Russia
• Department of Biochemistry, Biotechnology, and Microbiology, Far Eastern Federal University, Vladivostok 690090, Russia

autor

Tyunin A.P.

• Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, Vladivostok 690022, Russia

Bibliografia

• Aleynova OA, Grigorchuk VP, Dubrovina AS, Rybin VG, Kiselev KV (2016) Stilbene accumulation in cell cultures of Vitis amurensis Rupr. overexpressing VaSTS1, VaSTS2, and VaSTS7 genes. Plant Cell Tissue Organ Cult 125:329–339
• Boyko A, Zemp F, Filkowski J, Kovalchuk I (2006) Double-strand break repair in plants is developmentally regulated. Plant Physiol 141:488–497
• Boyko A, Golubov A, Bilichak A, Kovalchuk I (2010) Chlorine ions but not sodium ions alter genome stability of Arabidopsis thaliana. Plant Cell Physiol 51:1066–1078
• Britt AB (1996) DNA damage and repair in plants. Ann Rev Plant Physiol Plant Mol Biol 47:75–100
• Buss LW (1983) Evolution, development, and the units of selection. Proc Natl Acad Sci USA 80:1387–1391
• Caetano-Anolles G (1999) High genome-wide mutation rates in vegetatively propagated bermudagrass. Mol Ecol 8:1211–1221
• Dubrovina AS, Kiselev KV (2016) Age-associated alterations in the somatic mutation and DNA methylation levels in plants. Plant Biol (Stuttg) 18:185–196
• Dubrovina AS, Aleynova OA, Kiselev KV (2016) Influence of overexpression of the true and false alternative transcripts of calcium-dependent protein kinase CPK9 and CPK3a genes on the growth, stress tolerance, and resveratrol content in Vitis amurensis cell cultures. Acta Physiol Plant 38:78
• Golubov A, Yao Y, Maheshwari P, Bilichak A, Boyko A, Belzile F, Kovalchuk I (2010) Microsatellite instability in Arabidopsis increases with plant development. Plant Physiol 154:1415–1427
• Griffiths AJF, Miller JH, Suzuki DT et al (2000) An introduction to genetic analysis, 7th edn. W.H. Freeman, New York
• Hu ZB, Cools T, De Veylder L (2016) Mechanisms used by plants to cope with DNA damage. Annu Rev Plant Biol 67:439–462
• Jiang CF, Mithani A, Belfield EJ, Mott R, Hurst LD, Harberd NP (2014) Environmentally responsive genome-wide accumulation of de novo Arabidopsis thaliana mutations and epimutations. Genome Res 24:1821–1829
• Jin YH, Clark AB, Slebos RJC, Al-Refai H, Taylor JA, Kunkel TA, Resnick MA, Gordenin DA (2003) Cadmium is a mutagen that acts by inhibiting mismatch repair. Nat Genet 34:326–329
• Kiselev KV, Turlenko AV, Tchernoded GK, Zhuravlev YN (2009) Nucleotide substitutions in rolC and nptII gene sequences during long-term cultivation of Panax ginseng cell cultures. Plant Cell Rep 28:1273–1278
• Kiselev KV, Shumakova OA, Tchernoded GK (2011) Mutation of Panax ginseng genes during long-term cultivation of ginseng cell cultures. J Plant Physiol 168:1280–1285
• Kiselev KV, Dubrovina AS, Shumakova OA (2013) DNA mutagenesis in 2- and 20-year-old Panax ginseng cell cultures. In Vitro Cell Dev Biol Plant 35:1525–1532
• Kiselev KV, Tyunin AP, Ogneva ZV, Dubrovina AS (2015a) Age-associated alterations in somatic mutation level in Arabidopsis thaliana. Plant Growth Regul 75:493–501
• Kiselev KV, Dubrovina AS, Tyunin AP (2015b) The methylation status of plant genomic DNA influences PCR efficiency. J Plant Physiol 175:59–67
• Kiselev KV, Aleynova OA, Grigorchuk VP, Dubrovina AS (2017) Stilbene accumulation and expression of stilbene biosynthesis pathway genes in wild grapevine Vitis amurensis Rupr. Planta 245:151–159
• Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods 25:402–408
• Lombard DB, Chua KF, Mostoslavsky R, Franco S, Gostissa M, Alt FW (2005) DNA repair, genome stability, and aging. Cell 120:497–512
• Moskalev AA, Shaposhnikov MV, Plyusnina EN, Zhavoronkov A, Budovsky A, Yanai H, Fraifeld VE (2013) The role of DNA damage and repair in aging through the prism of Koch-like criteria. Ageing Res Rev 12:661–684
• Müller J, Sigel RK, Lippert B (2000) Heavy metal mutagenicity: insights from bioinorganic model chemistry. J Inorg Biochem 79:261–265
• Noro Y, Takano-Shimizu T, Syono K, Kishima Y, Sano Y (2007) Genetic variations in rice in vitro cultures at the EPSPs–RPS20 region. Theor Appl Genet 114:705–711
• Ossowski S, Schneeberger K, Lucas-Lledó JI, Warthmann N, Clark RM, Shaw RG, Weigel D, Lynch M (2010) The rate and molecular spectrum of spontaneous mutations in Arabidopsis thaliana. Science 327:92–94
• Pla M, Jofré A, Martell M, Molinas M, Gómez J (2000) Large accumulation of mRNA and DNA point modifications in a plant senescent tissue. FEBS Lett 472:14–16
• Smith KC (1992) Spontaneous mutagenesis: experimental, genetic and other factors. Mutat Res 277:139–162
• Spampinato CP (2017) Protecting DNA from errors and damage: an overview of DNA repair mechanisms in plants compared to mammals. Cell Mol Life Sci 74:1693–1709
• Tyunin AP, Kiselev KV (2016) Alternations in VaSTS gene cytosine methylation and t-resveratrol production in response to UV-C irradiation in Vitis amurensis Rupr. cells. Plant Cell Tissue Organ Cult 124:33–45
• Tuteja N, Singh MB, Misra MK, Bhalla PL, Tuteja R (2001) Molecular mechanisms of DNA damage and repair: progress in plants. Crit Rev Biochem Mol Biol 36:337–397
• Ulm R, Baumann A, Oravecz A, Máté Z, Adám E, Oakeley EJ, Schäfer E, Nagy F (2004) Genome-wide analysis of gene expression reveals function of the bZIP transcription factor HY5 in the UV-B response of Arabidopsis. Proc Natl Acad Sci USA 101:1397–1402
• Vijg J (2004) Impact of genome instability on transcription regulation of aging and senescence. Mech Ageing Dev 125:747–753
• Vijg J, Suh Y (2013) Genome instability and aging. Annu Rev Physiol 75:645–668
• Willing EM, Piofczyk T, Albert A, Winkler JB, Schneeberger K, Pecinka A (2016) UVR2 ensures transgenerational genome stability under simulated natural UV-B in Arabidopsis thaliana. Nat Commun 7:13522
• Yao Y, Kovalchuk I (2011) Abiotic stress leads to somatic and heritable changes in homologous recombination frequency, point mutation frequency and microsatellite stability in Arabidopsis plants. Mutat Res Fundam Mol Mech Mutagen 707:61–66

Identyfikatory

DOI

10.1007/s11738-017-2600-9