The effect of exogenous and endogenous phytohormones on the in vitro development of gametophyte and sporophyte in Asplenium nidus L.

• Autorzy: Menendez V. , Abdul Y. , Bohanec B. , Lafont F. , Fernandez H.
• Języki publikacji: EN

Abstrakty

EN

The fern Asplenium nidus L. is in great demand as an ornamental plant. The aim of this work was to investigate the influence of phytohormones in promoting a gametophytic and sporophytic growth in homogenized sporophytes tissue. Exogenous application of 0.5 and 5 µM N⁶-benzyladenine, 0.05 and 0.5 µM indole-3-acetic acid (IAA), and 0.3 and 3 µM gibberellic acid (GA3) favoured sporophyte regeneration, whereas gametophyte regeneration took place when plant material was cultured in a hormone-free liquid MS medium. The endogenous contents of the auxin IAA, the cytokinins trans-zeatin, transzeatin riboside, dihydrozeatin, dihydrozeatin riboside, isopentenyladenine and isopentenyladenosine, and the gibberellins GA₁, GA₃, GA₄, GA₇, GA₉ and GA₂₀ in growing gametophytes and sporophytes were evaluated. Similar levels of the auxin and cytokinins and qualitative differences in the gibberellins were found between both generations.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 6
• Opis fizyczny: p.2493-2500,fig.,ref.

Twórcy

autor

Menendez V.

• Lab of Plant Physiology, Department of BOS, c) Catedratico R Uria s/n, 33071 Oviedo, Spain

autor

Abdul Y.

• Lab of Plant Physiology, Department of BOS, c) Catedratico R Uria s/n, 33071 Oviedo, Spain

autor

Bohanec B.

• Centre for Plant Biotechnology and Breeding, Biotechnical Faculty, Jamnikarjeva 101, 1111 Ljubljana, Slovenia

autor

Lafont F.

• Mass Spectrometry Unit, Edificio Ramon y Cajal, Campus Rabanales, Cordova, Spain

autor

Fernandez H.

• Lab of Plant Physiology, Department of BOS, c) Catedratico R Uria s/n, 33071 Oviedo, Spain

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