Plant regeneration from carrot (Daucus carota L.) anther culture derived embryos

• Autorzy: Gorecka K. , Krzyzanowska D. , Kiszczak W. , Kowalska U.
• Języki publikacji: EN

Abstrakty

EN

The research concerned of the regeneration of plants from embryos obtained from anther cultures of seven carrot (Daucus carota L.) cultivars. The aim was to determine the influence of the regeneration medium on the efficiency of the regeneration process. The optimization of the adaptation of the obtained plants was also carried out. Embryogenesis occurred on four of the tested media: B₅ and MS without hormones, MS with charcoal, and MS with 1 mg dm⁻³ BA and 0.001 mg dm⁻³ NAA. Embryos obtained from the anther cultures produced secondary embryos, from which the regenerations of plants was observed. Secondary embryos were formed most extensively on the B₅ medium without hormones. The efficiency of the regeneration process depended on the cultivar. Most of the secondary embryos were formed by androgenetic embryos of the cultivar ‘Feria F₁’. The highest number of plants (102) regenerated from one embryo during 12 weeks of culture was also obtained in case of the cultivar ‘Feria F₁’. Secondary embryogenesis and plant regeneration from embryos allow to omit the difficult stage of root induction applied when plants are regenerated form shoots’ explants. This makes the plant regeneration process quicker and easier. The plants regenerated by the conversion of embryos are better adapted to the ex vitro conditions than those obtained in the two-stage organogenesis involving the regeneration of shoots and in second stage roots induction.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2009
• Tom: 31
• Numer: 6
• Opis fizyczny: p.1139-1145,fig.,ref.

Twórcy

autor

Gorecka K.

• Research Institute of Vegetable Crops, Kanstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland

autor

Krzyzanowska D.

• Research Institute of Vegetable Crops, Kanstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland

autor

Kiszczak W.

• Research Institute of Vegetable Crops, Kanstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland

autor

Kowalska U.

• Research Institute of Vegetable Crops, Kanstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland

Bibliografia

• Andersen SB, Christiansen J, Farestveit B (1990) Carrot (Daucus carota L.): in vitro production of haploids and field trials. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 12. Springer, Berlin, pp 393–402
• Bajaj YPS (1990) In vitro production of haploids and their use in cell genetics and plant breeding. In: Biotechnology in agriculture and forestry, vol 12. Springer, Berlin, pp 3–44
• Chauvin JE, Yang Q, Le Jeune B, Herve Y (1993) Obtention d’embrions par culture d’antheres chez le chou-fleur et le brocoli et evaluation des potentialites du materiel obtenu pour la creation varietale. Agronomie 13(7):579–590
• Chiang MS, Frechette S, Kuo CG, Chong C, Delafield SJ (1985) Embryogenesis and haploid plant production from anther culture of cabbage (Brassica oleracea var. capitata L.). Can J Plant Sci 65:1033–1037
• Dore C, Boulidard L (1988) Production de plantes androgenetiques de chou a choucroute (Brassica oleracea L. ssp. capitata) par culture d’anthers in vitro: comportement des lignees haploides doublees (HD) et leur interet comme parents d’hybrides F₁. Agronomie 8(10):51–862
• Gamborg OL, Miller RA, Ojima O (1968) Nutrient requirements of suspension cultures of soybean root cell. Exp Cell Res 50:151–158
• Górecka K (1998) Obtaining of homozygous lines of head cabbage (Brassica oleracea L. var. capitata L.) with aid of anther cultures. Habil qualifying thesis. Research Institute of Vegetable Crops, Skierniewice (Polish with English summary)
• Górecka K, Krzyżanowska D, Śmiech M, Hoser – Krauze J (1996) The effect of genotype on androgenesis and regeneration of plants from anther-derived embryos in brussels sprout. J Appl Genet 37A:191–194
• Górecka K, Krzyżanowska D, Górecki R (2005a) The influence of several factors on the efficiency of androgenesis in carrot. J of Appl Genet 46(3):265–269
• Górecka K, Krzyżanowska D, Kiszczak W, Górecki R (2005b) Embryo induction in anther culture. Veget Crops Res Bull 63:25–32
• Górecki R, Górecka K, Krzyżanowska D, Kozik E, Nowak R, Adasik B (2001) Rooting and adaptation of androgenic carrot plants. Veget Crops Res Bull 54:25–28
• Keller WA (1984) Anther culture of Brassica. In: Vasil IK (ed) Cell culture and somatic cell genetics of plant. Academic Press, New York, pp 302–310
• Keller WA, Armstrong KC (1977) Embryogenesis and plant regeneration in Brassica napus anther cultures. Can J Bot 55:1383–1388
• Keller WA, Armstrong KC (1978) High frequency of microsporederived plants from Brassica napus anther cultures. Z Pflanzenzücht 80:100–108
• Keller WA, Rajhathy T, Lacapra J (1975) In vitro production of plants from pollen in Brassica campestris. Can J Genet Cytol 17:655–666
• Lelu MA, Bollon H (1990) Cabbage Brassica oleracea var. capitata and Brussels sprouts Brassica oleracea var. gemmifera: In vitro production of haploids. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry 12. Springer-Verlag, Berlin, pp 358–373
• Matsubara S, Dohya N, Murakami K, Nishio T, Dore C (1995) Callus formation and regeneration of adventitious embryos from carrot, fennel and mitsuba microspores by anther and isolated microspore cultures. Acta Hort 392:129–137
• Möllers C, Iqbal MCM (2009) Doubled haploids in breeding winter oilseed rape. In: Touraev A, Forster BP, Mochan Jain S (eds) Advances in haploid production in higher plants. Springer Science?Business Media B.V., pp 161–169
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
• Nałęczyńska A (1991) The use of double haploids in rape-seed breeding. Part 1: obtaining homozygotic lines. Habil qualifying thesis. Plant Breeding and Acclimatization Institute, Radzików (Polish with English summary)
• Takahata Y, Keller WA (1991) High frequency embryogenesis and plant regeneration in isolated microspore culture of Brassica oleracea L. Plant Sci Limerick 74, 2:235–242
• Tyukavin GB, Shmykova NA, Monakhova MA (1999) Cytological study of embryogenesis in cultured carrot anthers. Russ J Plant Physiol 46(6):767–773

Uwagi

Rekord w opracowaniu