EN
Studies on the possibility of application of the attenuated strain of DHV (designated as P-50) for duckling immunization against duck virus hepatitis (DVH) have been undertaken. It has been shown that minimal dose of the virus immunizing 100 per cent of ducklings was 10 3.4 EID₅₀. Strain P-50 was reisolated from the liver of vaccinated ducklings and was excreted with faeces up to 9 days postvaccination. During 10 passages in susceptible ducklings, the reversion to virulent form of the attenuated strain have not been noted. The attenuated strain P-50 of DVH induced a quicker immunization effect and higher level of neutralizing antibodies in one-day-old ducklings than vaccinal strain E-52.