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2008 | 58 | 4 |

Tytuł artykułu

Chromatographic separation of tannin fractions from a bearberry-leaf (Arctostaphylos uva-ursi L. Sprengel) extract by SE-HPLC - a short report

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EN

Abstrakty

EN
Phenolic compounds from the leaves of the bearberry plant (Arctostaphylos uva-ursi L. Sprengel) were extracted into 95% (v/v) ethanol. Tannin fractions were obtained by Sephadex LH-20 column chromatography using 50% (v/v) acetone as the mobile phase. The tannin fraction was then further characterised by size exclusion high-performance liquid chromatography (SE-HPLC) on a TSK G2000SWXL column with a mobile phase comprising 45% acetonitrile and 0.1% (v/v) TFA. The chromatography revealed the presence of additional phenolic compounds in the tannin fractions which are not proanthocyanidins (i.e. condensed tannins).

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-

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Tom

58

Numer

4

Opis fizyczny

p.485-490,fig.,ref.

Twórcy

autor
  • Department of Food Science and Technology, The University of Georgia, 100 Cedar Street, Athens, GA, 30602-7610, USA
autor
autor

Bibliografia

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  • 15. Pegg R.B., Amarowicz N., Naczk M., Antioxidant activity of polyphenolics from a bearberry-leaf (Arctostaphylos uva-ursi L. Sprengel) extract in meat systems. ACS Symp. Series 909, 2005, 67–82.
  • 16. Pegg R.B., Amarowicz R., Naczk M., Shahidi F., PHOTOCHEM® for determination of antioxidant capacity of plant extracts. ACS Symp. Series 956, 2007, 140–158.
  • 17. Simonot D.L., Bio-Manufacturing in Saskatchewan. Assessment of the Manufacturing Potential of Select Saskatchewan Plants. 2000, Saskatchewan Nutraceutical Network, pp. 23–26.
  • 18. Slaveska-Raicki R., Rafajlovska V., Rizova V., Spirevska I., HPTLC determination of gallic acid and tannin in extracts of bearberry leaves. J. Planar Chromatogr., 2003, 16, 396–401.
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