Systematic analysis of genes involved in oral cancer metastasis to lymph nodes

• Autorzy: Zhang X. , Zhang T. , Tang X. , Lin Y. , Han X. , Wang H. , Ming H. , Liu K. , Feng G.
• Języki publikacji: EN

Abstrakty

EN

Oral cancer remains a deadly disease worldwide. Lymph node metastasis and invasion is one of the causes of death from oral cancer. Elucidating the mechanism of oral cancer lymph node metastasis and identifying critical regulatory genes are important for the treatment of this disease. This study aimed to identify differentially expressed genes (gene signature) and pathways that contribute to oral cancer metastasis to lymph nodes. The GSE70604-associated study compared gene profiles in lymph nodes with metastasis of oral cancer to those of normal lymph nodes. The GSE2280-associated study compared gene profiles in primary tumor of oral cancer with lymph node metastasis to those in tumors without lymph node metastasis. There are 28 common differentially expressed genes (DEGs) showing consistent changes in both datasets in overlapping analysis. GO biological process and KEGG pathway analysis of these 28 DEGs identified the gene signature CCND1, JUN and SPP1, which are categorized as key regulatory genes involved in the focal adhesion pathway. Silencing expression of CCND1, JUN and SPP1 in the human oral cancer cell line OECM-1 confirmed that those genes play essential roles in oral cancer cell invasion. Analysis of clinical samples of oral cancer found a strong correlation of these genes with short survival, especially JUN expression associated with metastasis. Our study identified a unique gene signature – CCND1, JUN and SPP1 – which may be involved in oral cancer lymph node metastasis.

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2018
• Tom: 23
• Opis fizyczny: p.1-14,fig.,ref.

Twórcy

autor

Zhang X.

• Institute of Tissue Engineering and Stem Cells, Nanchong Central Hospital, The Second Clinical Medical College of North Sichuan Medical College, No. 95, People’s south Road, Shunqing District, Nanchong, Sichuan 637000, People’s Republic of China

autor

Zhang T.

• Institute of Tissue Engineering and Stem Cells, Nanchong Central Hospital, The Second Clinical Medical College of North Sichuan Medical College, No. 95, People’s south Road, Shunqing District, Nanchong, Sichuan 637000, People’s Republic of China

autor

Tang X.

• Institute of Tissue Engineering and Stem Cells, Nanchong Central Hospital, The Second Clinical Medical College of North Sichuan Medical College, No. 95, People’s south Road, Shunqing District, Nanchong, Sichuan 637000, People’s Republic of China

autor

Lin Y.

• Institute of Tissue Engineering and Stem Cells, Nanchong Central Hospital, The Second Clinical Medical College of North Sichuan Medical College, No. 95, People’s south Road, Shunqing District, Nanchong, Sichuan 637000, People’s Republic of China

autor

Han X.

• Institute of Tissue Engineering and Stem Cells, Nanchong Central Hospital, The Second Clinical Medical College of North Sichuan Medical College, No. 95, People’s south Road, Shunqing District, Nanchong, Sichuan 637000, People’s Republic of China

autor

Wang H.

• Institute of Tissue Engineering and Stem Cells, Nanchong Central Hospital, The Second Clinical Medical College of North Sichuan Medical College, No. 95, People’s south Road, Shunqing District, Nanchong, Sichuan 637000, People’s Republic of China

autor

Ming H.

• Institute of Tissue Engineering and Stem Cells, Nanchong Central Hospital, The Second Clinical Medical College of North Sichuan Medical College, No. 95, People’s south Road, Shunqing District, Nanchong, Sichuan 637000, People’s Republic of China

autor

Liu K.

• Institute of Tissue Engineering and Stem Cells, Nanchong Central Hospital, The Second Clinical Medical College of North Sichuan Medical College, No. 95, People’s south Road, Shunqing District, Nanchong, Sichuan 637000, People’s Republic of China

autor

Feng G.

• Institute of Tissue Engineering and Stem Cells, Nanchong Central Hospital, The Second Clinical Medical College of North Sichuan Medical College, No. 95, People’s south Road, Shunqing District, Nanchong, Sichuan 637000, People’s Republic of China

Bibliografia

• 1. Werning JW. Oral Cancer: Diagnosis, Management, and Rehabilitation2007 May 16; 2007.
• 2. Collaborators GMaCoD. Global, regional, and national age-sex specific all-cause and cause-specific mortality for 240 causes of death, 1990-2013: a systematic analysis for the global burden of disease study 2013. Lancet. 2015;385(9963):117–71.
• 3. Noguti J, De Moura CF, De Jesus GP, Da Silva VH, Hossaka TA, Oshima CT, et al. Metastasis from oral cancer: an overview. Cancer Genomics Proteomics. 2012;9(5):329–35.
• 4. Liu X, Kolokythas A, Wang J, Huang H, Zhou X. Gene expression signatures of lymph node metastasis in Oral Cancer: molecular characteristics and clinical significances. Curr Cancer Ther Rev. 2010;6(4):294–307.
• 5. Civantos F, Zitsch R, Bared A. Sentinel node biopsy in oral squamous cell carcinoma. J Surg Oncol. 2007;96(4):330–6.
• 6. Civantos F Jr, Zitsch R, Bared A, Amin A. Sentinel node biopsy for squamous cell carcinoma of the head and neck. J Surg Oncol. 2008;97(8):683–90.
• 7. Kashiwazaki H, Hassan NM, Hamada J, Moriuchi T, Yamazaki Y, Tei K, et al. Gene expression profile changes correlated with lymph node metastasis in oral squamous cell carcinoma. Odontology. 2008;96(1):38–43.
• 8. Kato Y, Uzawa K, Saito K, Nakashima D, Kato M, Nimura Y, et al. Gene expression pattern in oral cancer cervical lymph node metastasis. Oncol Rep. 2006;16(5):1009–14.
• 9. Kondoh N, Ishikawa T, Ohkura S, Arai M, Hada A, Yamazaki Y, et al. Gene expression signatures that classify the mode of invasion of primary oral squamous cell carcinomas. Mol Carcinog. 2008;47(10):744–56.
• 10. Nagata M, Fujita H, Ida H, Hoshina H, Inoue T, Seki Y, et al. Identification of potential biomarkers of lymph node metastasis in oral squamous cell carcinoma by cDNA microarray analysis. Int J Cancer. 2003;106(5):683–9.
• 11. Nguyen ST, Hasegawa S, Tsuda H, Tomioka H, Ushijima M, Noda M, et al. Identification of a predictive gene expression signature of cervical lymph node metastasis in oral squamous cell carcinoma. Cancer Sci. 2007;98(5):740–6.
• 12. O'Donnell RK, Kupferman M, Wei SJ, Singhal S, Weber R, O'Malley B, et al. Gene expression signature predicts lymphatic metastasis in squamous cell carcinoma of the oral cavity. Oncogene. 2005;24(7):1244–51.
• 13. Roepman P, Wessels LF, Kettelarij N, Kemmeren P, Miles AJ, Lijnzaad P, et al. An expression profile for diagnosis of lymph node metastases from primary head and neck squamous cell carcinomas. Nat Genet. 2005;37(2):182–6.
• 14. Schmalbach CE, Chepeha DB, Giordano TJ, Rubin MA, Teknos TN, Bradford CR, et al. Molecular profiling and the identification of genes associated with metastatic oral cavity/pharynx squamous cell carcinoma. Arch Otolaryngol Head Neck Surg. 2004;130(3):295–302.
• 15. Warner GC, Reis PP, Jurisica I, Sultan M, Arora S, Macmillan C, et al. Molecular classification of oral cancer by cDNA microarrays identifies overexpressed genes correlated with nodal metastasis. Int J Cancer. 2004;110(6):857–68.
• 16. Watanabe H, Mogushi K, Miura M, Yoshimura R, Kurabayashi T, Shibuya H, et al. Prediction of lymphatic metastasis based on gene expression profile analysis after brachytherapy for early-stage oral tongue carcinoma. Radiother Oncol. 2008;87(2):237–42.
• 17. Zhou X, Temam S, Oh M, Pungpravat N, Huang BL, Mao L, et al. Global expression-based classification of lymph node metastasis and extracapsular spread of oral tongue squamous cell carcinoma. Neoplasia. 2006;8(11):925–32.
• 18. Fuste NP, Fernandez-Hernandez R, Cemeli T, Mirantes C, Pedraza N, Rafel M, et al. Cytoplasmic cyclin D1 regulates cell invasion and metastasis through the phosphorylation of paxillin. Nat Commun. 2016;7:11581.
• 19. Zhong Z, Yeow WS, Zou C, Wassell R, Wang C, Pestell RG, et al. Cyclin D1/cyclin-dependent kinase 4 interacts with filamin a and affects the migration and invasion potential of breast cancer cells. Cancer Res. 2010;70(5):2105–14.
• 20. Li Z, Jiao X, Wang C, Ju X, Lu Y, Yuan L, et al. Cyclin D1 induction of cellular migration requires p27(KIP1). Cancer Res. 2006;66(20):9986–94.
• 21. Meng H, Tian L, Zhou J, Li Z, Jiao X, Li WW, et al. PACSIN 2 represses cellular migration through direct association with cyclin D1 but not its alternate splice form cyclin D1b. Cell Cycle. 2011;10(1):73–81.
• 22. Chen YW, Huang HS, Shieh YS, Ma KH, Huang SH, Hueng DY, et al. A novel compound NSC745885 exerts an anti-tumor effect on tongue cancer SAS cells in vitro and in vivo. PLoS One. 2014;9(8):e104703.
• 23. Trapnell C, Williams BA, Pertea G, Mortazavi A, Kwan G, van Baren MJ, et al. Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat Biotechnol. 28(5):511–5.
• 24. Tavazoie S, Hughes JD, Campbell MJ, Cho RJ, Church GM. Systematic determination of genetic network architecture. Nat Genet. 1999;22(3):281–5.
• 25. Oka R, Nakashiro K, Goda H, Iwamoto K, Tokuzen N, Hamakawa H. Annexin A8 is a novel molecular marker for detecting lymph node metastasis in oral squamous cell carcinoma. Oncotarget. 2016;7(4):4882–9.
• 26. Rickman DS, Millon R, De Reynies A, Thomas E, Wasylyk C, Muller D, et al. Prediction of future metastasis and molecular characterization of head and neck squamous-cell carcinoma based on transcriptome and genome analysis by microarrays. Oncogene. 2008;27(51):6607–22.
• 27. Zenz R, Scheuch H, Martin P, Frank C, Eferl R, Kenner L, et al. c-Jun regulates eyelid closure and skin tumor development through EGFR signaling. Dev Cell. 2003;4(6):879–89.
• 28. Zhao C, Qiao Y, Jonsson P, Wang J, Xu L, Rouhi P, et al. Genome-wide profiling of AP-1-regulated transcription provides insights into the invasiveness of triple-negative breast cancer. Cancer Res. 2014;74(14):3983–94.
• 29. Eferl R, Ricci R, Kenner L, Zenz R, David JP, Rath M, et al. Liver tumor development. C-Jun antagonizes the proapoptotic activity of p53. Cell. 2003;112(2):181–92.
• 30. Xu H, Jin X, Yuan Y, Deng P, Jiang L, Zeng X, et al. Prognostic value from integrative analysis of transcription factors c-Jun and Fra-1 in oral squamous cell carcinoma: a multicenter cohort study. Sci Rep. 2017;7(1):7522.
• 31. Shevde LA, Samant RS. Role of osteopontin in the pathophysiology of cancer. Matrix Biol. 2014;37:131–41.
• 32. Subramani VN, Narasimhan M, Thiyagarajan M, Munuswamy BD, Jayamani L. Expression of Osteopontin in Oral squamous cell carcinoma and its surgical margins-an Immunohistochemical study. J Clin Diagn Res. 2015;9(11):ZC66–9.
• 33. Luo SD, Chen YJ, Liu CT, Rau KM, Chen YC, Tsai HT, et al. Osteopontin involves cisplatin resistance and poor prognosis in Oral squamous cell carcinoma. Biomed Res Int. 2015;2015:508587.
• 34. Anborgh PH, Mutrie JC, Tuck AB, Chambers AF. Pre- and post-translational regulation of osteopontin in cancer. J Cell Commun Signal. 2011;5(2):111–22.
• 35. Shi Z, Mirza M, Wang B, Kennedy MA, Weber GF. Osteopontin-a alters glucose homeostasis in anchorage-independent breast cancer cells. Cancer Lett. 2014;344(1):47–53.
• 36. Body S, Esteve-Arenys A, Miloudi H, Recasens-Zorzo C, Tchakarska G, Moros A, et al. Cytoplasmic cyclin D1 controls the migration and invasiveness of mantle lymphoma cells. Sci Rep. 2017;7(1):13946.

Identyfikatory

DOI

10.1186/s11658-018-0120-2