The intracellular survival of Campylobacter has been described within epithelial cells as well as in macrophages in vitro. The goal of this study was to estimate the viability of the genetically diverse C. jejuni and C. coli strains in the macrophage J774 cell line. Strains selected for analysis differed with regard to the occurrence of genes encoding specific virulence factors. The present work indicates that was no correlation between the source of isolates and relative intracellular survival.
Beltinger J, del Buono J, Skelly MM, Thornley J, Spiller RC, Stack WA, Hawkey CJ (2008) Disruption of colonic barrier function and induction of mediator release by strains of Campylobacter jejuni that invade epithelial cells. World J Gastroenterol 14: 7345- 7352.
Cooker AO, Isokpehi RD, Thomas BN, Amisu KO, Obi CL (2002) Human campylobacteriosis in developing countries. Emerg Infect Dis 8: 237-244.
Day WA Jr., Sajecki JL, Pitts TM, Joens LA (2000) Role ofcatalase in Campylobacter jejuni intracellular survival. Infect Immun 68: 6337-6345.
Krutkiewicz A, Klimuszko D (2010) Genotyping and PCR detection of potential virulence genes in Campylobacter jejuni and Campylobacter coli isolates from different sources in Poland. Folia Microbiol 55: 167-75.
Mixter PF, Klena JD, Flom GA, Siegesmund AM, Konkel ME (2003) In vivo tracking of Campylobacter jejuni by using a novel recombinant expressing green fluorescent protein. Appl Environ Microbiol 69: 2864-2874.
Pogacar M, Mihaljevic R, Klancnik A, Brumini G, Abram M, Mozina S (2008) Survival of stress exposed Campylobacter jejuni in the murine macrophage J774 cell line. Int J Food Microbiol 129: 68-73.
Smith CK, Kaiser P, Rothwell L, Humphrey T, Barrow PA, Jones MA (2005) Campylobacter jejuni-induced cytokine responses in avian cells. Infect Immun73: 2094-2100.
Watson RO, Galan JE (2008) Campylobacter jejuni survives within epithelial cells by avoiding delivery to lysosomes. PLoS Pathog 4: e 14
Young KT, Lindsay M.D, DiRita VJ (2007) Campylobacter jejuni: molecular biology and pathogenesis. Nat Rev Microbiol 5: 665-679.