Micropropagation of strawberry (Fragaria × ananassa Duch.) on chemically sterilized media

• Autorzy: Siekierzynska A. , Litwinczuk W.
• Języki publikacji: EN

Abstrakty

EN

Micropropagation of strawberry (Fragaria × ananassa Duch.) is a widely used technique to produce plantlets in commercial horticulture. Unfortunately, in contrast to propagation based on runners, it is also an expensive method and may lead to obtaining plants with changed phenotype. In order to reduce such problems, the influence of chemical method of medium sterilization (application of commercial home bleach – ACE®) instead of autoclaving on the development of in vitro cultures of four strawberry cultivars (‘Elsanta’, ‘Elkat’, ‘Senga Sengana’, ‘Polka’) was examined. The presented study proved that sodium hypochlorite added into the hot medium is very effective, making aseptic environment agent. Applied in tested dose (2 cm3 of ACE® per dm–3) is not harmful to strawberry in vitro cultures and usually causes positive changes in their development (reduced formation of adventitious shoots and callus), except for ‘Elsanta’ cultivar. Thus the application of media sterilized with sodium hypochlorite is beneficial and cost effective and may be recommended both for commercial and especially amateur strawberry micropropagation.

Słowa kluczowe

EN

plant breeding; strawberry; Fragaria x ananassa; Elsanta cultivar; Elkat cultivar; Polka cultivar; Senga Sengana cultivar; fruit plant; micropropagation; in vitro culture; sodium hypochlorite; medium; sterilization

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2018
• Tom: 17
• Numer: 3
• Opis fizyczny: p.147-156,fig.,ref.

Twórcy

autor

Siekierzynska A.

• Chair of Plant Physiology and Biotechnology, Faculty of Biology and Agriculture, University of Rzeszow, Cwiklinskiej 2, 35-601 Rzeszow, Poland

autor

Litwinczuk W.

• Chair of Plant Physiology and Biotechnology, Faculty of Biology and Agriculture, University of Rzeszow, Cwiklinskiej 2, 35-601 Rzeszow, Poland

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Identyfikatory

DOI

10.24326/asphc.2018.3.15