Proteinase inhibitors from Cajanus platycarpus accessions active against pod borer Helicoverpa armigera

• Autorzy: Swathi M. , Mohanraj S.S. , Swaroop V. , Gujjarlapudi M. , Mallikarjuna N. , Dutta-Gupta A. , Padmasree K.
• Języki publikacji: EN

Abstrakty

EN

Cajanus platycarpus, a wild relative of Cajanus cajan, is an important source for various agronomically desirable traits, including resistance towards pod borer, Helicoverpa armigera. In the present study, the inhibitory activity of proteinase inhibitors (PIs) present in crude protein extracted from different accessions of C. platycarpus and cultivars of C. cajan was evaluated against H. armigera under in vitro and in vivo conditions. The Pis active against H. armigera gut trypsin-like proteinases (HGPs), referred to as ‘HGPIs’, were more pronounced in mature dry seeds of C. platycarpus accessions when compared with cultivars, which is also evident through gelatin activity staining studies. Therefore, the inhibitory activity of HGPIs was further evaluated in various plant organs of C. platycarpus accessions, such as leaves, flowers, pods, developing seeds at 8–10 days (DAP-I), 18–20 days (DAP-II), and 28–32 days after pollination (DAP-III). However, the HGPI activity was more pronounced in mature dry seeds[DAP-III[DAP-II[DAPI[flowers[pods[leaves. The observed quantitative allocation of HGPIs closely resembled ‘‘Optimal Defense Theory’’. Further, bioassays demonstrated that there was a significant reduction in the body weight of the larvae fed upon crude PI extracts of C. platycarpus accessions with concomitant increase in mortality rate and the formation of larval–pupal intermediates. Nevertheless, such changes were not observed when the larvae were fed on crude PI extracts of C. cajan cultivars. These results suggest that the PI gene(s) from C. platycarpus accessions could be exploited in the management of H. armigera by introgression into C. cajan cultivars.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 11
• Opis fizyczny: Article: 242 [11 p.], fig.,ref.

Twórcy

autor

Swathi M.

• Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India

autor

Mohanraj S.S.

• Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India

autor

Swaroop V.

• Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, Hyderabad, 500 046, India

autor

Gujjarlapudi M.

• Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, Hyderabad, 500 046, India

autor

Mallikarjuna N.

• Legumes Cell Biology, Grain Legumes Program, ICRISAT, Patancheru, Hyderabad, India

autor

Dutta-Gupta A.

• Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad, India

autor

Padmasree K.

• Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, Hyderabad, 500 046, India

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Identyfikatory

DOI

10.1007/s11738-015-1991-8