Ocena kompetencji rozwojowej oocytów oraz zarodków świń na podstawie badań molekularnych i mikrofluidycznych

• Autorzy: Kempisty B. , Walczak R. , Sniadek P. , Dziuban J. , Piotrowska H. , Zawierucha P. , Zaorska K. , Antosik P. , Bukowska D. , Jaskowski J. M. , Nowicki M. , Zabel M.

Warianty tytułu

EN

Assessment of porcine oocytes and embryo developmental competence based on molecular and microfluidic research

• Języki publikacji: PL

Abstrakty

EN

Evaluation of oocyte developmental competence has an important influence on the ability of these cells to attain maturation, successful fertilization, development of embryo to the blastocyst stage and proper implantation. Factors determining the reproductive potential of gametes included: (1) expression of important transcription factors, (2) epigenetic changes, which influence the silencing of selected genes transcription, (3) transcription regulation, and (4) post-transcriptional regulation. The epigenetic changes mainly include: DNA methylation, histones modifications and changes in chromatin structure in the oocytes. In several studies, the association between oocyte morphology (mostly determined by cumulus cell layers and granularity of cytoplasm) and the ability of these cells to attain maturation and fertilization has been described. The use of biochemical, metabolomical and molecular markers is the most frequently applied tool in the assessment of oocyte developmental competence. However, most of these methods are invasive and lead to the decreased viability of the analyzed cells. Searching for new, objective and noninvasive techniques leads to the development of a microfluidic chip system, which shows the physical (spectral) properties of oocytes and embryos in comparison to the biological parameters. In this article, selected issues associated with the genetic regulation of such processes as: maturation of oocytes, fertilization and early stages of embryonic development, have been presented. Moreover, the regulation of transcription and post-transcriptional modification during oogenesis and embryogenesis in mammals, with special relation to pigs and the possibilities of applying of microfluidics in assessment of oocyte and embryo developmental competence was shown.

Słowa kluczowe

PL

ssaki; rozrod zwierzat; biologia rozrodu; trzoda chlewna; oocyty; zarodki; dojrzewanie oocytow; kompetencja rozwojowa; ocena; badania molekularne; badania mikrofluidyczne; mikroczipy fluidyczne; technologia Lab-on-Chip

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2011
• Tom: 67
• Numer: 10
• Opis fizyczny: s.673-677,rys.,bibliogr.

Twórcy

autor

Kempisty B.

• Katedra i Zakład Histologii i Embriologii, Wydział Lekarski II, Uniwersytet Medyczny w Poznaniu, ul.Święcickiego 6, 60-781 Poznań

autor

Walczak R.

autor

Sniadek P.

autor

Dziuban J.

autor

Piotrowska H.

autor

Zawierucha P.

autor

Zaorska K.

autor

Antosik P.

autor

Bukowska D.

autor

Jaskowski J. M.

autor

Nowicki M.

autor

Zabel M.

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