Determinaton of enrofloxacin and ciprofloxacin in albumin and freeze-dried-eggs by liquid chromatography with fluorescence detection

• Autorzy: Gdybik-Sikorska M. , Posyniak A. , Gajda A. , Bladek T.
• Języki publikacji: EN

Abstrakty

EN

The study presents a method for determination of enrofloxacin and ciprofloxacin in albumin and freeze-dried-eggs with the use of liquid chromatography with fluorescence detection. The procedure enables a simple isolation of fluoroquinolones from albumin based on extraction with acetonitrile, and from freeze-dried-eggs with acetonitrile under alkaline conditions. The samples have been analysed on ultracarb C8 liquid chromatography column. The gradient elution programmes consisted of the mixture of 0.03 M phosphoric acid with 0.002 M sodium 1-heptanesulfonate monohydrate and acetonitrile. The method has been validated according to requirements of the European Decision 2002/657/EC. Recoveries ranged from 78% to 83% for spiked freeze-dried-eggs and from 89% to 91% for albumin. The developed method can be applied for determination and confirmation of enrofloxacin and ciprofloxacin in albumin and freeze-dried-eggs.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2013
• Tom: 57
• Numer: 3
• Opis fizyczny: p.351-355,fig.,ref.

Twórcy

autor

Gdybik-Sikorska M.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Posyniak A.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Gajda A.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Bladek T.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

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Identyfikatory

DOI

10.2478/bvip-2013-0061