Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2002 | 07 | 2A |

Tytuł artykułu

The genetic structure of tetraploid Avena: a comparison of isozyme and RAPD markers

Warianty tytułu

Języki publikacji



Isozymes were the first widely used molecular markers in plant population analysis. They yielded valuable information on the amount and the structure of genetic variability. DNA technology has provided new types of markers based on DNA sequence, which make it possible to study polymorphisms in a much greater proportion of the genome. This is the reason why the use of isozymes is less popular nowadays. This effect would be justified if all markers provided the same type of information on polymorphism and genetic relationships among populations; otherwise, it would be necessary to use different markers to obtain the complete picture of the genetic structure of populations and species. In this study, we compared data of isozyme and RAPD markers in the populations of two tetraploid species of wild oats: Avena barbata populations collected in Argentina, and Avena murphyi populations collected in Spain and Morocco. The samples were evaluated for 9 isozymatic systems and 10 primers. The structure of genetic variability was studied using Nei’s method, and the relationships between populations were estimated using Hedrick and Jaccard's similarities for isozymes and RAPDs, respectively. As expected, RAPDs were more polymorphic than isozymes, but the information obtained from both markers was weakly correlated. The various reasons for this observation are discussed, but our conclusion is that in order to study the structure of genetic variability, several types of markers should be used.








Opis fizyczny



  • Departement de Biologie, Faculte des Sciences, Universite Ibn Tofail, 14000, Kenitra, Morocco
  • Departamento de Genetica, Facultad de Biologia, Universidad de Leon, 24071 Leon, Spain
  • Departamento de Genetica, Facultad de Biologia, Universidad de Leon, 24071 Leon, Spain
  • Departamento de Genetica, Facultad de Biologia, Universidad de Leon, 24071 Leon, Spain
  • Departamento de Genetica, Facultad de Biologia, Universidad de Leon, 24071 Leon, Spain


  • 1. Ladizinsky, G. and Zohary, D. Genetic relationships between diploids and tetraploids in series Eubarbatae of Avena. Can. J. Genet. Cyto. 10 (1968) 68-81.
  • 2. Ladizinsky, G. Avena murphyi: A new tetraploid species of oat from Southern Spain. Israel J. Bot. 20 (1971) 24-27 .
  • 3. Legget, J. M., Ladizinsky, G., Hagberg, P. and Obanni, M. The distribution of nine Avena species in Spain and Morocco. Can. J. Bot. 70 (1992) 240- 244.
  • 4. Hutchinson, E. S., Hakim Elahi, A., Miller, R. D. and Allard R. W. The genetics of the diploidized tetraploid Avena barbata. J. Hered. 74 (1983) 325-330.
  • 5. Allard, R. W., García, P., Sáenz de Miera, L. E. and Pérez de la Vega, M. Evolution of Multilocus Genetic Structure in Avena hirtula and Avena barbata. Genetics 135 (1993) 1125-1139.
  • 6. Brook, G. M. Plant DNA isolation. Molecular Genetic Analysis of Populations. Oxford University Press, 1992, 72-37.
  • 7. Álvarez, M. T., García P. and Pérez de la Vega, M. RAPD polymorphism in Spanish lentil landraces and cultivars. J. Genet. Breed. 51 (1997) 91-96.
  • 8. Nei, M. Analysis of gene diversity in subdivided populations. Proc. Nat. Acad. Sci. USA. 70 (1973) 3321-3323.
  • 9. Szidt, A. E., Wang, X. R. and Lu, M. Z. Empirical assessment of allozyme and RAPD variation in Pinus sylvestris (L.) using haploid tissue analysis. Heredity 6 (1996) 412-420.
  • 10. Heun, M., Murphy, J. P. and Philips, T. D. A comparison of RAPD and isozyme analyses for determining the genetic relationships among Avena sterilis L. accessions. Theor. Appl. Genet. 87 (1994) 689-696.

Typ dokumentu



Identyfikator YADDA

JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.