Typing of Listeria Monocytogenes isolated from slaughtered cattle and beef meat

• Autorzy: Dmowska K. , Wieczorek K. , Lynch O. , Osek J.
• Języki publikacji: EN

Abstrakty

EN

A total of 135 L. monocytogenes strains isolated from slaughtered cattle and beef meat were tested by the pulsed field gel electrophoresis (PFGE). The Asel restriction analysis revealed a genetic heterogeneity among investigated isolates since 31,9, and 35 profiles were distinguished among hide, carcass, and meat strains, respectively. The PFGE profiles of the isolates were also analysed in relation to serotypes, virulence genes, and antimicrobial resistance. It was shown that strains displaying the same PFGE type were of the same serotype while correlation between pulsotype and antimicrobial resistance was poor. The obtained results suggest that a cross-contamination between bovine hides and carcasses may occur during the slaughter process. Moreover, identification of identical PFGE types among L. monocytogenes found during a study period may suggest a common source of contamination or presence of persistent strains able to survive for a long time. These results emphasise the importance of molecular subtyping methods, including PFGE, in monitoring and tracking pathogen contamination along food chain.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2013
• Tom: 57
• Numer: 2
• Opis fizyczny: p.179-183,ref.

Twórcy

autor

Dmowska K.

• Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Wieczorek K.

• Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Lynch O.

• Department of Hygiene of Food of Animal Origin Teagasc Food Research Centre-Ashtown, Ashtown, Dublin 15, Ireland

autor

Osek J.

• Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, 24-100 Pulawy, Poland

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