Subtilisin-like serine proteases involved in N remobilization during grain filling in wheat

• Autorzy: Roberts I.N. , Caputo C. , Kade M. , Criado M. V. , Barneix A. J.
• Języki publikacji: EN

Abstrakty

EN

The induction of two subtilisin-like proteases (P1 and P2) associated with stress-induced senescence in young plants was investigated in adult wheat plants during the grain-filling period. Western blot analysis of flag leaf extracts showed that P1 was induced very late in the life cycle of the plants (9 days post-anthesis) and that 7 days later it reached a 2.5-fold increase with respect to the initial value at anthesis. On the other hand, the P2 signal was already detected previous to anthesis and increased soon after anthesis, reaching a fourfold increase by the end of the grain-filling period. The induction of P1 and P2 temporally correlates with the degradation of the Rubisco small and large subunits in the flag leaf, as well as with nitrogen (N) accumulation in the ears. At the same time, a decrease in the endogenous concentration of the cytokinins isopentenyladenine and isopentenyladenosine (iP + iPA) in the leaves was observed. In detached leaves senescing in the dark, the levels of both proteases were affected by 6-benzylaminopurine application: the induction of P1 was completely prevented, whereas the induction of P2 was reduced. Our findings demonstrate that both P1 and P2 are expressed in leaves of adult plants and are induced during natural senescence. These results enable us to postulate their participation in N remobilization to developing grains during monocarpic senescence and their regulation by a cytokinin-mediated mechanism.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 5
• Opis fizyczny: p.1997-2001,fig.,ref.

Twórcy

autor

Roberts I.N.

• Facultad de Agronomía, Instituto de Investigaciones en Biociencias Agricolas y Ambientales (INBA), Universidad de Buenos Aires, Av. San Martin 4453, C1417DSE, Buenos Aires, Argentina

autor

Caputo C.

• Facultad de Agronomía, Instituto de Investigaciones en Biociencias Agricolas y Ambientales (INBA), Universidad de Buenos Aires, Av. San Martin 4453, C1417DSE, Buenos Aires, Argentina

autor

Kade M.

• Facultad de Agronomía, Instituto de Investigaciones en Biociencias Agricolas y Ambientales (INBA), Universidad de Buenos Aires, Av. San Martin 4453, C1417DSE, Buenos Aires, Argentina

autor

Criado M. V.

• Facultad de Agronomía, Instituto de Investigaciones en Biociencias Agricolas y Ambientales (INBA), Universidad de Buenos Aires, Av. San Martin 4453, C1417DSE, Buenos Aires, Argentina

autor

Barneix A. J.

• Instituto de Suelos, CIRN INTA, De los Reseros y Nicolas Repetto 1686, Hurlingham, Provincia de Buenos Aires, Argentina

Bibliografia

• Arnon DI (1949) Cooper enzymes in chloroplasts. Polyphenoloxydases in Beta vulgaris. Plant Physiol 24:1–15
• Bernard SM, Moller ALB, Dionisio G, Kichey T, Jahn TP, Dubois F, Baudo M, Lopes MS, Tercé-Laforgue T, Foyer CH, Parry MAJ, Forde BG, Araus JL, Hirel B, Schjoerring JK, Habash DZ (2008) Gene expression cellular localisation and function of glutamine synthetase isozymes in wheat (Triticum aestivum L.). Plant Mol Biol 67:89–105
• Bradford MM (1976) A rapid and sensitive method for the quantitation of micrograms quantities of proteins utilizing the principle of protein–dye binding. Anal Biochem 72:248–254
• Buchanan-Wollaston V, Earl S, Harrison E, Mathas E, Navabpour S, Page T, Pink D (2003) The molecular analysis of leaf senescence—a genomics approach. Plant Biotechnol J 1:3–22
• Cregan PB, van Berkum P (1984) Genetics of nitrogen metabolism and physiological/biochemical selection for increased grain crop productivity. Theor Appl Genet 67:97–111
• Criado MV, Roberts IN, Echeverria M, Barneix AJ (2007) Plant growth regulators and induction of leaf senescence in nitrogendeprived wheat plants. J Plant Growth Regul 26:301–307
• Feller U, Fischer A (1994) Nitrogen metabolism in senescing leaves. Crit Rev Plant Sci 13:241–273
• Feller U, Anders I, Mae T (2008) Rubiscolytics: fate of Rubisco after its enzymatic function in a cell is terminated. J Exp Bot 59:1615–1624
• Gan S, Amasino RM (1997) Making sense of senescence. Molecular genetic regulation and manipulation of leaf senescence. Plant Physiol 113:313–319
• Gregersen PL, Holm PB, Krupinska K (2008) Leaf senescence and nutrient remobilisation in barley and wheat. Plant Biol 10:37–49
• Hoagland DR, Arnon DI (1950) The water culture method for growing plants without soil. Calif Agric Exp Stn Circ 347:1–32
• Kamachi K, Yamaya T, Mae T, Ojima K (1991) A role for glutamine synthetase in the remobilization of leaf nitrogen during natural senescence in rice leaves. Plant Physiol 96:411–417
• Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
• Lawlor DW, Kontturi M, Young AT (1989) Photosynthesis by flag leaves of wheat in relation to protein, ribulose bisphosphate carboxylase activity and nitrogen supply. J Exp Bot 40:43–52
• Martínez DE, Bartoli CG, Grbic V, Guiamet JJ (2007) Vacuolar cysteine proteases of wheat (Triticum aestivum L.) are common to leaf senescence induced by different factors. J Exp Bot 58:1099–1107
• Parrott D, Yang L, Shama L, Fischer AM (2005) Senescence is accelerated, and several proteases are induced by carbon ‘‘feast’’ conditions in barley (Hordeum vulgare L.) leaves. Planta 222:989–1000
• Peoples MB, Dalling MJ (1988) The interplay between proteolysis and amino acid metabolism during senescence and nitrogen reallocation. In: Noodén LD, Leopold AC (eds) Senescence and aging in plants. Academic Press, San Diego, pp 181–217
• Reed A, Below FE, Hageman RH (1980) Grain protein accumulation and the relationship between leaf nitrate reductase and protease activities during grain development in maize (Zea mays L.) I. Variation between genotypes. Plant Physiol 66:164–170
• Roberts IN, Ferna´ndez Murray P, Caputo CP, Passeron S, Barneix AJ (2003) Purification and characterization of a subtilisin-like serine protease induced during the senescence of wheat leaves. Physiol Plant 118:483–490
• Roberts IN, Passeron S, Barneix AJ (2006) The two main endoproteases present in dark-induced senescent wheat leaves are distinct subtilisin-like proteases. Planta 224:1437–1447
• Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from acrylamide gels to nitrocellulose sheets: procedure and applications. Proc Natl Acad Sci USA 76:4350–4354
• Ueda T, Seo S, Ohashi Y, Hashimoto J (2000) Circadian and senescence-enhanced expression of a tobacco cysteine protease gene. Plant Mol Biol 44:649–657

Uwagi

PL

Rekord w opracowaniu