Andrographolide loaded montmorillonite attenuated enterotoxigenic Escherichia coli induced intestinal barrier injury and inflammation in a mouse model

• Autorzy: Wang P. , Li L. , Gan L. , Chen Q. , Qiao H. , Gao W. , Zhang Y. , Wang J.
• Języki publikacji: EN

Abstrakty

EN

Montmorillonite (MMT), a natural absorbent agent, has widely been accepted for its antidiarrhea function in human and farm animals; however, its specific physicochemical property limits its biological function in practical use. In the current study, raw MMT was loaded by andrographolide, namely andrographolide loaded montmorillonite (AGP-MMT). The microstructure of AGP-MMT was observed by scanning electron microscope (SEM) and X-ray diffraction (XRD). The effect of AGP-MMT on the growth performance, intestinal barrier and inflam- mation was investigated in an enterotoxigenic Escherichia coli (ETEC) challenged mice model. The results show that the microstructure of MMT was obviously changed after andrographolide modification: AGP-MMT exhibited a large number of spheroid particles, and floccule aggregates, but lower interplanar spacing compared with MMT. ETEC infection induced body weight losses and intestinal barrier function injury, as indicated by a lower villus height and ratio of villus height/crypt depth, whereas the serum levels of diamine oxidase (DAO), D-xylose and ETEC shedding were higher in the ETEC group compared with the CON group. Mice pretreated with AGP-MMT showed alleviated body weight losses and the intestinal barrier function injury induced by ETEC challenge. The villus height and the ratio of villus height/crypt depth, were higher in mice pretreated with AGP-MMT than those pretreated with equal levels of MMT. Pretreatment with AGP-MMT also alleviated the increased concentration of serum tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), and the corresponding genes in the jejunum induced by ETEC infection in mice. The protein and mRNA levels of IL-1β were lower in mice pretreated with AGP-MMT than those with equal levels of MMT. The results indicate that AGP-MMT was more effective in alleviating intestinal barrier injury and inflammation in mice with ETEC challenge than MMT.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2023
• Tom: 26
• Numer: 3
• Opis fizyczny: p.367-376,fig.,rerf.

Twórcy

autor

Wang P.

• College of Biology Engineering, Henan University of Technology, Zhengzhou, China

autor

Li L.

• College of Biology Engineering, Henan University of Technology, Zhengzhou, China

autor

Gan L.

• College of Biology Engineering, Henan University of Technology, Zhengzhou, China

autor

Chen Q.

• College of Biology Engineering, Henan University of Technology, Zhengzhou, China

autor

Qiao H.

• College of Biology Engineering, Henan University of Technology, Zhengzhou, China

autor

Gao W.

• College of Biology Engineering, Henan University of Technology, Zhengzhou, China

autor

Zhang Y.

• College of Biology Engineering, Henan University of Technology, Zhengzhou, China

autor

Wang J.

• College of Biology Engineering, Henan University of Technology, Zhengzhou, China

Identyfikatory

DOI

10.24425/pjvs.2023.145042