Identification and antimicrobial susceptibility of microbial agents of otitis externa in dogs

• Autorzy: Kahya Demirbilek S. , Yilmaz O.
• Języki publikacji: EN

Abstrakty

EN

In this study, a total of 277 unmedicated dogs with otitis externa were used. Overall, 413 agents were isolated from 277 ear swab samples; 52.7% of the cases were mono-infections (146 cases), and 37.1% of the cases were poly-infections (103 cases). In 10.1% (28) of the cases, neither bacteria nor yeasts were isolated. Coagulase-positive Staphylococcus spp. were the most frequently isolated bacteria and were found in 90 (21.8%) of the samples. Fifty-eight samples, (14%) were positive for Staphylococcus aureus, 51 (12.3%) for Pseudomonas aeruginosa, 27 (6.5%) for Proteus mirabilis, 27 (6.5%) for Malassezia pachydermatis, 21 (5%) for Corynebacterium spp., 21 (5%) for β-haemolytic Streptococcus spp., 15 (3.6%) for Staphylococcus pseudointermedius, 12 (2.9%) for Proteus spp., 12 (2.9%) for Escherichia coli, 9 (2.1%) for Acinetobacter calcoaceticus, 7 (1.6%) for Trichophyton mentagrophytes, 5 (1.2%) for Staphylococcus auricularis, and 46 (11.1%) for different bacteria and yeasts. A total of 14 different bacteria and yeasts were isolated and identified. Kirby-Bauer antibiotic susceptibility testing was carried out for 10 different antibiotics. The bacterial isolates were found to be resistant to amoxicillin-clavulanic acid (45%), gentamycin (28%), ampicillin/cloxacillin (69%), tobramycin (28%), amikacin (23%), enrofloxacin (47%), chloramphenicol (58%), doxycycline (65%), lincomycin/spectinomycin (58%) and polymyxin B (62%). In conclusion, it is important to test the antimicrobial sensitivity of aetiological agents of otitis externa before treatment so as to prevent the development of antibiotic resistance in bacteria and yeasts.

Słowa kluczowe

EN

animal disease; veterinary medicine; dog; otitis externa; swimmer's ear zob.otitis externa; identification; bacterial isolation; yeast; antimicrobial susceptibility; microbial agent

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2019
• Tom: 75
• Numer: 02
• Opis fizyczny: p.107-110,ref.

Twórcy

autor

Kahya Demirbilek S.

• Department of Microbiology, Faculty of Veterinary Medicine, Uludag University, 16.059, Gorukle-Bursa, Turkey

autor

Yilmaz O.

• Department of Microbiology, Faculty of Veterinary Medicine, Uludag University, 16.059, Gorukle-Bursa, Turkey

Bibliografia

• August J. R.: Otitis externa. A disease of multifactorial etiology. Vet. Clin. North Am. Small Anim. Pract. 1988, 18, 731-742.
• Borum A. E., Çeçen G., Demir G., Çetin C., Şentürk S.: Determination and antimicrobial susceptibility of microorganisms isolated from otitis externa with dogs. Kocatepe Vet. Derg. 2014, 7, 27-31.
• CLSI (Clinical Laboratory Standard Institute): National committee for clinical and laboratory standards and methods for antimicrobial susceptibility testing of anaerobic bacteria, Approved standard. 6th edition, NCCLS, Wayne, PA, Document M11-A6, 2004.
• Griffin C. E.: Classifying cases of otitis externa the PPSP System. Pro. ESVD Workshop on Otitis. St Helens 2010.
• Kamaljyoti A. K. S., Sood N. K., Sharma S., Filia G.: Epidemiology and diagnosis of mycotic infections in canine otitis externa. Intas Polivet 2016, 17, 302-303.
• Mande J. D., Kitaa J. M. A.: Microbial profile and antimicrobial susceptibility of isolates from dogs with otitis externa in Kenya. J. Kenya Vet. Assoc. 2005, 29, 40-44.
• Moraes L. A., Pereira J. M. M., Silva S. P., Moreira V. M. T., Casseb A.: Microbiological diagnosis and bacterial multiresistance in vitro of external otitis externa of dogs. Veterinaria Zootecnia 2014, 21, 98-101.
• Murugan M. S., Parthiban S., Malmarugan S., Rajeshwar J. J.: Antibiogram and therapeutic management of bacterial otitis externa: A clinical study of 81 dogs. Intas Polivet 2016, 17, 292-294.
• Nardoni S., Ebani V. V., Fratini F., Mannella R., Pinferi G., Mancianti F., Finotello R., Perrucci S.: Malessezia, mites and bacteria in the external ear canal of dogs and cats with otitis externa. Slov. Vet. Res. 2014, 51, 113-118.
• Öztürk D., Pehlivanoğlu F., Türütoğlu H., Şirin Y. S., Şababoğlu E.: Otitis externalı köpeklerden izole edilen mikroorganizmalar ve antibiyotik duyarlılıkları. Euro. J. Vet. Sci. 2016, 32, 84-88.
• Paterson S.: Discovering the causes of otitis externa. In Practice, Focus 2016. http://dx.doi.org/10.1136/inp.i470.
• Perry L. R., Maclennan B., Korven R., Rawlings T. A.: Epidemiological study of dogs with otitis externa in Cape Breton, Nova Scotia. Can. Vet. J. 58, 168-174.
• Petrov V., Mihaylov G., Tsachev I., Zhelev G., Marutsov P., Koev K.: Otitis externa in dogs: microbiology and antimicrobial susceptibility. Rev. Med. Vet. 2013, 164, 18-22.
• Quinn P. J., Carter M. E., Markey B.: Section: 2 Bacteriology, [in:] Clinical Veterinary Microbiology. Harcourt Publishers Limited, USA 1999, p. 118-254.
• Rosser E. J.: Causes of otitis externa. Vet. Clinics Small Animal. 2004, 34, 459-468.
• Rosychuk R. A. W.: Management of otitis externa. Vet. Clin. North Am. Small Anim. Pract. 1994, 24, 921-952.
• Sarıerler M., Kırkan Ş.: Microbial diagnosis and therapy of canine otitis externa. Veteriner Cerrahi Dergisi 2004, 10, 11-15.
• Selvakumar S., Mohamed A. M. G., Pothiappan P.: A clinical study of otitis externa with bacterial isolation, antimicrobial susceptibility and therapeutic management in 44 dogs. Intas Polivet 16, 332-333.
• Scott D. W., Miller W. H., Griffin C. E.: Otitis, [in:] Muller and Kirk’s Small Animal Dermatology. Saunders, Philadelphia 2001, p. 143-148.

Identyfikatory

DOI

10.21521/mw.6136