PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 23 | 5 |
Tytuł artykułu

PCB biodegradation and bphA1 gene expression induced by salicylic acid and biphenyl with pseudomonas fluorescence P2W and ralstonia eutropha H850

Autorzy
Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Organic carbon substrate amendments are promising bioremediation strategies to induce polychlorinated biphenyls (PCB) aerobic degradation. However, their selective induction on PCB degraders has not been well studied. In this study, the substrate interaction effects of salicylic acid and biphenyl on PCB biodegradation were investigated with pure cultured isolates, including a newly isolated Pseudomonas fluorescence (P2W) and the veteran PCB degrader Ralstonia eutropha (H850). A significant biodegradation of lower-chlorinated PCB in H850 was induced by both salicylic acid and biphenyl amendments, while the biodegradation in P2W was induced only by salicylic acid. The binary substrates of salicylic acid and biphenyl resulted in a significantly inhibited effect on PCB removal in both strains. The expression of the functional gene bphA1 in the upper biphenyl degradation pathway was further investigated by quantitative reverse transcription PCR. Compared to H850, P2W had higher expression in the bphA1 gene induced mainly by salicylic acid rather than biphenyl. Particularly, the binary substrate induction led to an excessive expression of bphA1 gene in both strains, which was in good agreement with their biomass growth. These results suggested that the special induction of PCB biodegradation depends on the selection of organic carbon substrates and the acclimation of degrader strains.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
23
Numer
5
Opis fizyczny
p.1591-1598,fig.,ref.
Twórcy
autor
  • Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China
autor
  • Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China
autor
  • Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China
autor
  • Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China
Bibliografia
  • 1. KIM J.S., KIM J.H., RYU E.K., KIM J.K., KIM C.K., HWANG I., LEE K. Versatile catabolic properties of Tn4371-encoded bph pathway in comamonas testosteroni (formerly Pseudomonas sp.) NCIMB 10643. J. Microbiol. Biotechnol. 14, 302, 2004.
  • 2. GRABOWSKA I. Polychlorinated biphenyls (PCBs) in Poland: occurrence, determination and degradation. Pol. J. Environ. Stud. 19, 7, 2010.
  • 3. URBANIAK M., SKOWRON A., FRATCZAK W., ZIELINSKI M., WESOLOWSKI W. Transport of polychlo- rinated biphenyls in urban cascade reservoirs: levels, sources and correlation to environmental conditions. Pol. J. Environ. Stud. 19, 201, 2010.
  • 4. OH E.T., KOH S.C., KIM E., AHN Y.H., SO J.S. Plant ter- penes enhance survivability of polychlorinated biphenyl (PCB) degrading Pseudomonas pseudoalcaligenes KF707 labeled with gfp in microcosms contaminated with PCB. J. Microbiol. Biotechnol. 13, 463, 2003.
  • 5. JIA L.Y., ZHENG A.P., XU L., HUANG X.D., ZHANG Q., YANG F.L. Isolation and characterization of comprehensive biphenyl-degrading bacterium, Enterobacter sp LY402. J. Microbiol. Biotechnol. 18, 952, 2008.
  • 6. WILLAMS W.A., LOBOS J.H., CHEETHAM W.E. A phy- logenetic analysis of aerobic polychlorinated biphenyl- degrading bacteria. Int. J. Syst. Bacteriol. 47, 207, 1997.
  • 7. SUENAGA H., GOTO M., FURUKAWA K. Active-site engineering of biphenyl dioxygenase: effect of substituted amino acids on substrate specificity and regiospecificity. Appl. Microbiol. Biotechnol. 71, 168, 2006.
  • 8. YANG X., LIU X., SONG L., XIE F., ZHANG G., QIAN S. Characterization and functional analysis of a novel gene cluster involved in biphenyl degradation in Rhodococcus sp. strain R04. J. Appl. Microbiol. 103, 2214, 2007.
  • 9. PARNELL J.J., DENEF V.J., PARK J., TSOI T., TIEDJE J.M. Environmentally relevant parameters affecting PCB degradation: carbon source- and growth phase-mitigated effects of the expression of the biphenyl pathway and asso­ciated genes in Burkholderia xenovorans LB400. Biodegradation. 21, 147, 2010.
  • 10. OHMORI T., MORITA H., TANAKA M., TOMOI M., MIYAUCHI K., KASAI D., FURUKAWA K., MASAI E., FUKUDA M. Expression in Escherichia coli of biphenyl 2,3-dioxygenase genes from a Gram-positive polychlorinat- ed biphenyl degrader, Rhodococcus jostii RHA1. Biosci. Biotechnol. Biochem. 75, 26, 2011.
  • 11. XUL., TENG Y., LI Z.G., NORTON J.M., LUO Y.M. Enhanced removal of polychlorinated biphenyls from alfal­fa rhizosphere soil in a field study: the impact of a rhizobial inoculum. Sci. Total Environ. 408, 1007, 2010.
  • 12. LUO W.S., D'ANGELO E.M., COYNE M.S. Plant sec­ondary metabolites, biphenyl, and hydroxypropyl P- cyclodextrin effects on aerobic polychlorinated biphenyl removal and microbial community structure in soils. Soil Biol. Biochem. 39, 735, 2007.
  • 13. LUO W.S., D'ANGELO E.M., COYNE M.S. Organic car­bon effects on aerobic polychlorinated biphenyl removal and bacterial community composition in soils and sedi­ments. Chemosphere 70, 364, 2008.
  • 14. SINGER A.C., SMITH D., JURY W.A., HATHUC K., CROWLEY D.E. Impact of the plant rhizosphere and aug­mentation on remediation of polychlorinated biphenyl contaminated soil. Environ. Toxicol. Chem. 22, 1998, 2003.
  • 15. IWASAKI T., TAKEDA H., MIYAUCHI K., YAMADA T., MASAI E., FUKUDA M. Characterization of two biphenyl dioxygenases for biphenyl/PCB degradation in a PCB degrader, Rhodococcus sp. strain RHA1. Biosci. Biotechnol. Biochem. 71, 993, 2007.
  • 16. SAKAI M., MASAI E., ASAMI H., SUGIYAMA K., KIM- BARA K., FUKUDA M. Diversity of 2,3- Dihydroxybiphenyl dioxygenase genes in a strong PCB degrader, Rhodococcus sp. strain RHA1. J. Biosci. Bioeng. 93, 421, 2002.
  • 17. YANG X.Q., XIE F.H., ZHANG G.Q., SHI Y.W., QIAN S.J. Purification, characterization, and substrate specificity of two 2,3-dihydroxybiphenyl 1,2-dioxygenase from Rhodococcus sp. R04 showing their distinct stability at var­ious temperature. Biochimie. 90, 1530, 2008.
  • 18. SHINDO K., OSAWA A., NAKAMURA R., KAGIYAMA Y., SAKUDA S., SHIZURI Y., FURUKAWA K., MISAWA N. Conversion from arenes having a benzene ring to those having a picolinic acid by simple growing cell reactions using Escherichia coli that expressed the six bacterial genes involved in biphenyl catabolism. J. Am. Chem. Soc. 126, 15042, 2004.
  • 19. SUENAGA H., WATANABE T., SATO M., NGADIMAN, FURUKAWA K. Alteration of regiospecificity in biphenyl dioxygenase by active-site engineering. J. Bacteriol. 184, 3682, 2002.
  • 20. PARK Y.I., SO J.S., KOH S.C. Induction by carvone of the polychlorinated biphenyl (PCB)-degradative pathway in Alcaligenes eutrophus H850 and its molecular monitoring. J. Microbiol. Biotechnol. 9, 804, 1999.
  • 21. ZHANG Y.C., HU C.H., LUO W.S. Influences of electron donor, bicarbonate and sulfate on bioreduction processes and manganese/copper redistributions among minerals in a water- saturated Sediment. Soil Sediment Contamin. 23, 94, 2014.
  • 22. SHI J.Y., LIN H.R., YUAN X.F., CHEN X.C., SHEN C.F., CHEN Y.X. Enhancement of copper Availability and micro­bial community changes in rice rhizospheres affected by sul­fur. Molecules 16, 1409, 2011.
  • 23. WANG Y.P., ZHU K., ZHENG Y.M., WANG H.T., DONG G.W., HE N., LI Q.B. The effect of recycling flux on the per­formance and microbial community composition of a biofilm hydrolytic-aerobic recycling process treating anthraquinone reactive dyes. Molecules 16, 9838, 2011.
  • 24. LUO W.S., HU C.H. Interactions of plant secondary metabolites and organic carbon substrates affected on biodegradation of polychlorinated biphenyl. J. Environ. Biol., 34, 337, 2013.
  • 25. BALDWIN B.R., NAKATSU C.H., NIES L. Detection and enumeration of aromatic oxygenase genes by multiplex and real-time PCR. Appl. Environ. Microbiol. 69, 3350, 2003.
  • 26. PARNELL J.J., PARK J., DENEF V., TSOI T., HASHSHAM S., QUENSEN J., TIEDJE J.M. Coping with polychlorinated biphenyl (PCB) toxicity: physiological and genome-wide responses of Burkholderia xenovorans LB400 to PCB-mediated stresss. Appl. Environ. Microbiol., 72, 6607, 2006.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-7763d323-1545-4f3b-ab08-29fbba7e9e5e
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.