Immunohistochemical identification and localisation of gastrin and somatostatin in endocrine cells of human pyloric gastric mucosa

• Autorzy: Kasacka I. , Lebkowski W. , Janiuk I. , Lapinska J. , Lewandowska A.
• Języki publikacji: EN

Abstrakty

EN

The detailed description of the distribution of endocrine cells G and D producing important hormones that regulate activation of other cells in the human stomach may be a valuable source of information for opinions about mucosa changes in different diseases of the alimentary tract. The density and distribution of immunoreactive G and D cells in the pylorus of humans (donors of organs) were evaluated. The pylorus samples were collected after other organs were harvested for transplantation. The number of G cells in the pyloric mucosa of healthy people was higher than the number of D cells. G and D cells were distributed between columnar cells of epithelium mucosa. Multiform endocrine cells generally occurred: gastrin in the middle third of the mucosa and somatostatin cells in the basal half of the pyloric mucosa. The investigation of the pyloric part of the healthy human stomach showed a characteristic distribution of cells that reacted with antisera against gastrin and somatostatin. (Folia Morphol 2012; 71, 1: 39–44)

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2012
• Tom: 71
• Numer: 1
• Opis fizyczny: p.39-44,fig.,ref.

Twórcy

autor

Kasacka I.

• Department of Histology and Cytophysiology, Medical University of Bialystok, ul.Kilinskiego 1, 15–089 Bialystok, Poland

autor

Lebkowski W.

• Department of Neurosurgery, Medical University of Bialystok, Poland

autor

Janiuk I.

• Department of Morphology Vertebrates, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland

autor

Lapinska J.

• Department of Histology and Cytophysiology, Medical University of Bialystok, ul.Kilinskiego 1, 15–089 Bialystok, Poland

autor

Lewandowska A.

• Department of Histology and Cytophysiology, Medical University of Bialystok, ul.Kilinskiego 1, 15–089 Bialystok, Poland

Bibliografia

• 1. Arnold R, Hülst MV, Neuhof CH, Schwarting H, Becker HD, Creutzfeldt W (1982) Antral gastrin-producing G-cells and somatostatin-producing D-cells in different states of gastric acid secretion. Gut, 23: 285–291.
• 2. Boehm BO (2003). The therapeutic potential of somatostatin receptor ligands in the treatment of obesity and diabetes. Expert Opin Investig Drugs, 12: 1501–1509.
• 3. Grandi D, Shenton FC, Chazot PL, Morini G (2008) Immunolocalization of histamine H3 receptors on endocrine cells in the rat gastrointestinal tract. Histol Histopathol, 23: 789–798.
• 4. Herman GE, Elfont EA (1991) The taming of immunohistochemistry: the new era of quality control. Biotech Histochem, 66: 194–199.
• 5. Joseph IM, Zavros Y, Merchant JL, Kirschner D (2003) A model for integrative study of human gastric acid secretion. J Appl Physiol, 94: 1602–1618.
• 6. Kanai S, Hosoya H, Akimoto S, Ohta M, Matsui T, Takiguchi S, Funakoshi A, Miyasaka K (2009) Gastric acid secretion in cholecystokinin-1 receptor, -2 receptor, and -1, -2 receptor gene knockout mice. J Physiol Sci, 59: 23–29.
• 7. Kanno T, Matsuki T, Oka M, Utsunomiya H, Inada K, Magari H, Inoue I, Maekita T, Ueda K, Enomoto S, Iguchi M, Yanaoka K, Tamai H, Akimoto S, Nomoto K, Tanaka R, Ichinose M (2009) Gastric acid reduction leads to an alteration in lower intestinal microflora. Biochem Biophys Res Commun, 381: 666–670.
• 8. Kasacka I, Majewski M (2007) An immunohistochemical study of endocrine cells in the stomach of hypertensive rats. J Physiol Pharmacol, 3: 469–478.
• 9. Konturek SJ, Brzozowski T, Konturek PC, Schubert ML, Pawlik WW, Padol S, Bayner J (2008) Brain-gut and appetite regulating hormones in the control of gastric secretion and mucosal protection. J Physiol Pharmacol, 59 (suppl. 2): 7–31.
• 10. Lamberts SW, van der Lely AJ, de Herder WW, Hofland LJ (1996) Octreotide. NEJM, 334: 246–254.
• 11. Larsson LI, Tingstedt JE, Hougaard DM (1995) Coexpression of the gastrin and somatostatin genes in differentiating and neoplastic human cells. Histochem Cell Biol, 104: 139–144.
• 12. Liu Y, Vosmaer GD, Tytgat GN, Xiao SD, Ten Kate FJ (2005) Gastrin (G) cells and somatostatin (D) cells in patients with dyspeptic symptoms: Helicobacter pylori associated and non-associated gastritis. J Clin Pathol, 58: 927–931.
• 13. Low MJ (2004) Clinical endocrinology and metabolism. The somatostatin neuroendocrine system: physiology and clinical relevance in gastrointestinal and pancreatic disorders. Best Pract Res Clin Endocrinol Metab, 18: 607–622.
• 14. Raikhlin NT, Kvetnoi IM, Solomatina IM (1983) The APUD system and the hormonal basis of gastrointestinal activity. Sov Med, 6: 53–59.
• 15. Reidy DL, Schwartz JD (2004) Therapy for unresectable hepatocellular carcinoma: review of the randomized clinical trials-I: hepatic arterial embolization and embolization-based therapies in unresectable hepatocellular carcinoma. Anticancer Drugs, 15: 427–437.
• 16. Saffouri B, DuVal JW, Makhlouf GM (1984) Stimulation of gastrin secretion in vitro by intraluminal chemicals: regulation by intramural cholinergic and noncholinergic neurons. Gastroenterology, 87: 557–561.
• 17. Schubert ML (2008) Hormonal regulation of gastric acid secretion. Curr Gastroenterol Rep, 10: 523–527.
• 18. Schubert ML (2009) Gastric exocrine and endocrine secretion. Curr Opin Gastroenterol, 25: 529–536.
• 19. Schubert ML, Peura DA (2008) Control of gastric acid secretion in health and disease. Gastroenterology, 134: 1842–1860.
• 20. Solcia E, Rindi G, Buffa R, Fiocca R, Capella C (2000) Gastric endocrine cells: types, function and growth. Regul Pept, 93: 31–35.
• 21. Sun FP, Song YG (2003) G and D cells in rat antral mucosa: an immunoelectron microscopic study. World J Gastroenterol, 9: 2768–2771.
• 22. Timurkaan S, Timurkaan N, Ozkan E, Girgin M (2009) Immunohistochemical distribution of somatostatin, glucagon and gastrin in the gastric fundus of the citellus (Spermophilus xanthoprymnus) J Animal Vet Adv, 8: 2210–2214.
• 23. Tzaneva M (2004) Effects of duodenogastric reflux on gastrin cells, somatostatin cells and serotonin cells in human antral gastric mucosa. Pathol Res Pract, 200: 431–438.
• 24. Tzaneva MA (2003) Ultrastructural immunohistochemical localization of gastrin, somatostatin and serotonin in endocrine cells of human antral gastric mucosa. Acta Histochem, 105: 191–201.
• 25. Vinik AI, Gaginella TS, O’Dorisio TM, Shapiro B, Wagner L (1981) The distribution and characterization of somatostatin-like immunoreactivity in epithelial cells, submucosa, and muscle of the rat stomach and intestine. Endocrinology, 109: 1921–1926.
• 26. Waldum HL, Kvetnoi IM, Sylte R, Schulze B, Martinsen TC, Sandvik AK (1998) The effect of the peroxisome proliferator ciprofibrate on the gastric mucosa and articularly the gastrin cell. J Mol Endocrinol, 20: 111–117.
• 27. Waldum HL, Sandvik AK, Brenna E, Petersen H (1991) Gastrin-histamine sequence in the regulation of gastric acid secretion. Gut, 32: 698–701.
• 28. Xie XZ, Zhao ZG, Qi DS, Wang ZM (2006) Assay of gastrin and somatostatin in gastric antrum tissues of children with chronic gastritis and duodenal ulcer. World J Gastroenterol, 12: 2288–2290.
• 29. Zavros Y, Orr MA, Xiao C, Malinowska DH (2008) Sonic hedgehog is associated with H+-K+-ATPase-containing membranes in gastric parietal cells and secreted with histamine stimulation. Am J Physiol Gastrointest Liver Physiol, 295: 99–111.