Comparative 2-D electrophoresis of salivary proteins in Triatoma dimidiata and Rhodnius prolixus (Hemiptera: Reduviidae) and major cross-reactive antigens

• Autorzy: Monteon V. , May-Gil I. , Acosta-Viana K. , Ramos-Logonio A. , Hernandez O. , Lopez R.
• Języki publikacji: EN

Abstrakty

EN

An immune response to triatomine’s saliva is an immunological marker of exposure to triatomine bites. However, considerable variability in salivary protein profiles did exist among species. In the present work, we compare salivary proteins from Mexican Triatoma dimidiata and Rhodnius prolixus using 2-D electrophoresis. A clear differential saliva profile was found to exist between these two triatomine species. Fewer protein spots were detected in R. prolixus than in T. dimidiata. More than half of the proteins had an isoelectric point between 5 and 7 and a molecular weight between 10 and 30 kDa in T. dimidiata. Mice exposed to T. dimidiata saliva mount an immune response to three major cross-reacting antigens in R. prolixius saliva with weights of 10 kDa and 55 kDa. Our findings may alert for the presence of cross-reacting antigens between triatomine species in regions where two or more species are overlapping in the same geographical area.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2017
• Tom: 63
• Numer: 2
• Opis fizyczny: p.121-125,fig.,ref.

Twórcy

autor

Monteon V.

• Centro Investigaciones Biomedicas, Universidad Autonoma Campeche, Patricio Trueba s/n, Campeche 24090, Mexico

autor

May-Gil I.

• Centro Investigaciones Biomedicas, Universidad Autonoma Campeche, Patricio Trueba s/n, Campeche 24090, Mexico

autor

Acosta-Viana K.

• Biologia Celular, Centro Regional de Investigaciones Biomedicas, Universidad Autonoma Yucatan, Itzaes, Merida 97000, Mexico

autor

Ramos-Logonio A.

• LADISER Inmunología y Biología Molecular, Facultad de Ciencias Químicas, Universidad Veracruzana, Prolongacion Avenida Ote 1009, Orizaba 94340, Mexico

autor

Hernandez O.

• Centro Investigaciones Biomedicas, Universidad Autonoma Campeche, Patricio Trueba s/n, Campeche 24090, Mexico

autor

Lopez R.

• Centro Investigaciones Biomedicas, Universidad Autonoma Campeche, Patricio Trueba s/n, Campeche 24090, Mexico

Bibliografia

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Identyfikatory

DOI

10.17420/ap6302.95