Polymorphism of the beta-3-adrenergic receptor gene (ADRB3) and its distribution in domestic and wild pigs in China

• Autorzy: Wu J. , Liu J. , Yuan Y. , Qiao L. , Hao H. , Liu W.
• Języki publikacji: EN

Abstrakty

EN

The beta 3-adrenergic receptor (ADRB3) is a G-protein coupled receptor encoded by ADRB3 gene and involved in regulation of lipolysis and homeostasis. The objective of this study was to detect the genetic variation existed in this gene in eight domestic and wild pig populations. The polymorphism of partial sequences of ADRB3 gene in 336 pigs was analysed by single strand conformation polymorphism technique based on PCR (PCR-SSCP). Fst statistic and analysis of molecular variance (AMOVA) were used to measure the genetic differentiation. The genetic distances between populations were computed and a phylogenic tree was reconstructed by the unweighted pair group method using the arithmetic mean. Five haplotypes named A, B, C, D and E were detected with the frequencies ranging from 0.060 for E to 0.324 for A. The reliability of all haplotype frequencies reached 0.95. The haplotypes involved four polymorphic sites, of which one located in exon 2 with a thymine insertion, and the remaining three in non-coding regions with G/A and G/T substitutions, respectively. The thymine insertion resulted in the absence of two amino acids in the sequence of receptor protein. The haplotype diversity ranged from 0.579±0.057 in pure wild Liaoning to 0.792±0.024 in Mashen pigs, indicating abundant genetic diversity to exist in the populations. The Fst estimates showed that the degree of population differentiation between domestic and Wild pigs was moderate, but at a lower level within domestic populations. About 94.38% of the genetic variation originated from within the populations by AMOVA.

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2012
• Tom: 30
• Numer: 2
• Opis fizyczny: p.139-149,fig.,ref.

Twórcy

autor

Wu J.

• College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China

autor

Liu J.

autor

Yuan Y.

autor

Qiao L.

autor

Hao H.

autor

Liu W.

Bibliografia

• CANNON B., NEDERGAARD J., 2004 – Brown adipose tissue: function and physiological significance. Physiological Reviews 84(1), 277-359.
• CHANG H., NOZAWA K., LIU X.L., SUN W., GENG S.M., SHEN W., 2000 – On phylogenetic relationships among native goat populations along the middle and lower Yellow River Valley. Asian-Australian Journal of Animal Science 13(2), 137-148.
• CHIKUN K., HORIUCHI A., IDE H., SHIBATA M., HAYASHI T., NAKAJIMA I., OE M., MUROYA S., 2008 – Nucleotide sequence polymorphisms of beta1, beta2, and beta3-adrenergic receptor genes on Jinhua, Meishan, Duroc and Landrace pigs. Animal Science Journal 79(6), 665-672.
• COLLINS S., SURWIT R.S., 2001 – The beta-adrenergic receptors and the control of adipose tissue metabolism and thermogenesis. Recent Progress in Hormone Research 56, 309-328.
• EXCOFFIER L., LAVAL G., SCHNEIDER S., 2005 – Arlequin ver. 3.0: An integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online 1, 47-50.
• EXCOFFIER L., SMOUSE P.E., QUATTRO J.M., 1992 – Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131(2), 479-491.
• FAN B., WANG Z.G., LI Y.J., ZHAO X.L., LIU B., ZHAO S.H., YU M., L.I M.H., CHEN S.L.,XIONG T.A., LI K., 2002 – Genetic variation analysis within and among Chinese indigenous swine populations using microsatellite markers. Animal Genetics 33(6), 422-427.
• FANG M., HU X., JIANG T., BRAUNSCHWEIG M., HU L., DU Z., FENG J., ZHANG Q., WU C.,LI N., 2005 – The phylogeny of Chinese indigenous pig breeds inferred from microsatellite markers.Animal Genetics 36(1), 7-13.
• FORREST R.H., HICKFORG J.G., HOGAN A., FRAMPTON C., 2003 – Polymorphism at the ovine beta3-adrenergic receptor locus: associations with birth weight, growth rate, carcass composition and cold survival. Animal Genetics 34(1), 19-25.
• FORREST R.H., HICKFORD J.G., WYNYARD J., MERRICK N., HOGAN A., FRAMPTON C., 2006 – Polymorphism at the beta-adrenergic receptor (ADRB3) locus of Merino sheep and its association with lamb mortality. Animal Genetics 37(5), 465-468.
• GOUDET J., RAYMOND M., De MEEÜS T., ROUSSET F., 1996 – Testing differentiation in diploid populations. Genetics 144(4), 1933-1940.
• GOUDET J., 2001 – FSTAT, a Program to estimate and test gene diversities and fixation indices Version 2.9.3. Available from http://www.unil.ch/izea/softwares/ fstat.html
• KIM T.H., KIM K.S., CHOI B.H., YOON D.H., JANG G.W., LEE K.T., CHUNG H.Y., LEE H.Y., PARK H.S., LEE J.W., 2005 – Genetic structure of pig breeds from Korea and China Rusing microsatellite loci analysis. Journal of Animal Science 83(10), 2255-2263.
• KUROKAWA N., YOUNG E.H., OKA Y., SATOH H., WAREHAM N.J., SANDHU M.S., LOOS R.J.,2008 – The ADRB3 Trp64Arg variant and BMI: a meta-analysis of 44833 individuals. International Journal of Obesity 32(8), 1240-1249.
• MARVELLE A.F., LANGE L.A., QIN L., ADAIR L.S., MOHLKE K.L., 2008 — Association of FTO with obesity-related traits in the Cebu Longitudinal Health and Nutrition Survey (CLHNS)Cohort. Diabetes 57(7), 1987-1991.
• MERSMANN H.J., 1998 – Overview of the effects of beta-adrenergic receptor agonists on animal growth including mechanisms of action. Journal of Animal Science 76(1), 160-172.
• NAGANO T., MATSUDA Y., TANIOKA T., YOSHIOKA T., HIROI T., YOSHIKAWA K., OKABE K., OSAKA K., NAGAMINE I., TAKASAKA Y., 2005 – No association of the Trp 64 Arg mutation of the beta3-adrenergic receptor gene with obesity, type 2 diabetes mellitus, hyperlipidemia, and hypertension in Japanese patients with schizophrenia. The Journal of Medical Investigation 52(1-2), 57-64.
• NEI M., 1987 – Molecular Evolutionary Genetics. Columbia University Press, New York.
• OCHOA M.C., MARTI A., AZCONA C., CHUECA M., OYARZÁBAL M., PELACH R., PATINO A, MORENO-ALIAGA M.J., MARTÍNEZ-GONZÁLEZ M.A., MARTÍNEZ J.A., 2004 – Gene-gene interaction between PPAR gamma2 and ADRB3 increases obesity risk in children and adolescents.International Journal of Obesity and Related Metabolic Disorders 28 (Suppl 3), S37-41.
• RAMIS J.M., GONZALEZ-SANCHEZ J.L., PROENZA A.M., MARTÍNEZ-LARRAD M.T.,FERNÁNDEZ-PÉREZ C., PALOU A., SERRANO-RÍOS M., 2004 – The Arg64 allele of the beta 3-adrenoceptor gene but not the 3826G allele of the uncoupling protein 1 gene is associated with increased leptin levels in the Spanish population. Metabolism 53(11), 1411-1416.
• RANKINEN T., ZUBERT A., CHAGNON Y.C., WEISNAGEI S.J., ARGYROPOULOS G., WALTS B., PERUSSE L., BOUCHARD C., 2006 – The human obesity gene map: the 2005 update. Obesity 14(4), 529-644.
• REVELLI J.P., PREITNER F., SAMEC S., MUNIESA P., KUEHNE F., BOSS O., VASSALLI J.D., DULLOO A., SEYDOUX J., GIACOBINO J.P., HUARTE J., ODY C., 1997 – Targeted gene disruption reveals a leptin-independent role for the mouse beta3-adrenoceptor in the regulation of body composition. Journal of Clinical Investigation 100(5), 1098-1106.
• REYNOLDS J., WEIR B.S., COCKERHAM C.C., 1983 – Estimation for the coancestry coefficient:basis for a short-term genetic distance. Genetics 105(3), 767-779.
• RICE W.R., 1989 – Analyzing tables of statistical tests. Evolution 43(1), 223-225.
• SAMBROOK J., FRITSCH E.F., MANATIS T., 1989 – Molecular Cloning: a Laboratory Manual.2nd edn. Cold Spring Harbour Laboratory Press, New York.
• SMITH T.R., BIDWELL C.A., MILLS S.E. 2001, – Nucleotide sequence of the porcine beta-3 adrenergic receptor gene. Journal of Animal Science 79(3), 781-782.
• SOLLERO B.P., PAIVA S.R., FARIA D.A., GUIMARĂES S.E.F., BERTRANI G.R., MAMANI E.,ALBUQUERQUE M.S.M., CASTRO S.T.R., GERMANO J.L., MARIANTE A.S., 2006 – Genetic diversity in naturalized and commercial pig breeds in Brazil. 8th World Congress on Genetics Applied to Livestock Production, Belo Horizonte, MG, Brasil.
• STROSBERG A.D., 1997 – Structure and function of the beta 3-adrenergic receptor. Annual Review of Pharmacology and Toxicology 37, 421-450.
• TAMURA K., DUDLEY J., NEI M., KUMAR S., 2007 – MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24(8), 1596-1599.
• TANAKA K., IWAKI Y., TAKIZAWA T., MURAKAMI M., MANNEN H., MAEDA Y., KUROSAWA Y., DAND V.B., CHHUM PHITH L., BOUAHOM B., YAMAMOTO Y., DAING T., NAMIKAWA T., 2007 – The novel polymorphism of the beta 3-adrenergic receptor gene and its distribution In domestic pigs and wild boars in Asia. Animal Science Journal 78(3), 243-250.
• THUY N.T., MELCHINGER-WILD E., KUSS A.W., CUONG N.V., BARTENSCHLAGER H., GELDERMANN H., 2006 – Comparison of Vietnamese and European pig breeds Rusing microsatellites. Journal of Animal Science 84(10), 2601-2608.
• ZHANG G.X., WANG Z.G., SUN F.Z., CHEN W.S., YANG G.Y., GUO S.J., LI Y.J., ZHAO X.L.,ZHANG Y., SUN J., FAN B., YANG S.L., LI K., 2003 – Genetic diversity of microsatellite loci In fifty-six Chinese native pig breeds. Acta Genetica Sinica 30(3), 225-233. (In Chinese).

Uwagi

PL

Rekord w opracowaniu