Exploration of biochemical and molecular diversity in chickpea seeds to categorize cold stress-tolerant and susceptible genotypes

• Autorzy: Kaur S. , Arora M. , Gupta A. K. , Kaur N.
• Języki publikacji: EN

Abstrakty

EN

Chickpea (Cicer arietinum L.) genotypes are sensitive to low temperature (<10°C) during its reproductive stage suffer from abortion of flowers, infertile pods and small shriveled seeds that resulted in a significant decrease in crop yield. In the present investigation seeds of a number of cold stress-tolerant and susceptible genotypes were evaluated for biochemical and molecular diversity with the purpose to categorize them. The activities of various antioxidative enzymes (superoxide dismutase, glutathione reductase, ascorbate peroxidase and catalase), content of H₂O₂ and malondialdehyde, enzymes involved in phosphate metabolism (acid and alkaline phosphatases), and content of phytic acid and proline were determined in seeds of 20 cold stress tolerant and seven cold stress susceptible genotypes. Higher activities of superoxide dismutase, ascorbate peroxidase, catalase and acid phosphatase and low content of malondialdehyde and phytic acid were observed in cold stress-tolerant genotypes as compared to cold stress susceptible genotypes. Seventeen chickpea genotypes comprising both cold stress-tolerant and susceptible ones were evaluated through 20 randomly amplified polymorphic DNA (RAPD) primers. The results of cluster analysis revealed two major groups. In the first group five tolerant (group 1a) and six susceptible genotypes (group 1b) clustered together whereas in second group all the tolerant genotypes clustered together (group 2). Out of 20 RAPD primers, 4 primers (Opa-13, Opa-14, Opa-15 and Opa-16) have been identified as markers for cold stress tolerance. In general high SOD activity, and H₂O₂ content and low MDA and phytic acid content are related with cold stress tolerance. The status of these markers was more pronounced in genotypes clustered in group 2 after RAPD analysis than in group 1a of cold stress-tolerant genotypes as compared to susceptible genotypes. The observed biochemical and molecular diversity could be useful for identifying and developing cold stress-tolerant genotypes of chickpea.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 2
• Opis fizyczny: p.569-580,fig.,ref.

Twórcy

autor

Kaur S.

• Department of Biochemistry, Punjab Agricultural University, 141004 Ludhiana, India

autor

Arora M.

• Department of Biochemistry, Punjab Agricultural University, 141004 Ludhiana, India

autor

Gupta A. K.

• Department of Biochemistry, Punjab Agricultural University, 141004 Ludhiana, India

autor

Kaur N.

• Department of Biochemistry, Punjab Agricultural University, 141004 Ludhiana, India

Bibliografia

• Asada K (1999) The water–water cycle in chloroplasts: scavenging of active oxygen and dissipation of excess photons. Ann Rev Plant Physiol Plant Mol Biol 50:601–639
• Berger JD, Turner NC (2007) The ecology of chickpea: evolution, distribution, stresses and adaptation from an agro-climatic perspective. In: Yadav SS, Redden R, Chen W, Sharma B (eds) Chickpea breeding management. CABI, UK, pp 47–71
• Bowler C, van Montague M, Inzeé D (1992) Superoxide dismutase and stress tolerance. Ann Rev Plant Physiol Plant Mol Biol 43:83–116
• Clarke HJ, Siddique KHM, Khan TN (2005) Chickpea improvement in southern Australia: breeding for tolerance to chilling at flowering. Ind J Pulses Res 180:1–8
• Croser JS, Clarke HJ, Siddique KHM, Khan TN (2003) Low temperature stress: implications for chickpea (Cicer arietinum L.) improvement. Crit Rev Plant Sci 22:185–219
• Dat J, Vandenabeele S, Vranova E, Van Montagu M, Inze DF, Van Breusegem F (2000) Dual action of the active oxygen species during plant stress response. Cell Mol Life Sci 57:779–795
• Fogelman E, Kaplon A, Tanami Z, Ginzberg I (2011) Antioxidative activity associated with chilling injury tolerance of muskmelon (Cucumis melo L.). J Exp Bot 51:267–273
• Garg R, Patel RK, Tyagi AK, Jain M (2011) De novo assembly of chickpea transcriptome using short reads for gene discovery and marker identification. DNA Res 18:53–63
• Gechev TS, Breusegam FV, Stone JM, Denev I, Laloi C (2006) Reactive oxygen species as signals that modulate plant stress responses and programmed cell death. BioEssays 28:1091–1101
• Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. Arch Biochem Biophy 125:189–198
• Hernandez-Nistal J, Dopico B, Labrador E (2002) Cold and salt stress regulates the expression and activity of a chickpea cytosolic Cu/Zn superoxide dismutase. Plant Sci 163:507–514
• Jain D, Chattopadhyay D (2010) Analysis of gene expression in response to water deficit of chickpea (Cicer arietinum L.) varieties differing in drought tolerance. BMC Plant Biol 10:24
• Karp A (1997) Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories. Mol Breed 3:381–390
• Kaur V, Kaur N, Gupta AK (1999) Phosphatase activity and phosphorus partitioning in nodules of developing mungbean. Acta Physiol Plant 21:215–220
• Kaur S, Gupta AK, Kaur N, Sandhu JS, Gupta SK (2009a) Antioxidative enzymes and sucrose synthase contribute to cold stress tolerance in chickpea. J Agron Crop Sci 195:393–397
• Kaur H, Gupta AK, Kaur N, Sandhu JS (2009b) Differential response of antioxidative system in wild and cultivated varieties of chickpea. Plant Growth Regul 57:109–114
• Kingston SAH, Foyer CF (2000) Over expression of Mn-superoxide dismutase in maize leaves leads to increased monodehydroascorbate reductase, dehydroascorbate reductase and glutathione reductase activities. J Exp Bot 51:1867–1877
• Kumar S, Malik J, Thakur P, Kaistha S, Sharma KD, Upadhyay HD, Berger JD, Nayyar H (2011) Growth and metabolic responses of contrasting chickpea (Cicer arietinum L.) genotypes to chilling stress at reproductive phase. Acta Physiol Plant 33:779–787
• Lee SH, Ahsan N, Lee KW, Kim DH, Lee DG, Kwak SS, Kwon SY, Kim TH, Lee BH (2007) Simultaneous overexpression of both CuZn superoxide dismutase and ascorbate peroxidase in transgenic tall fescue plants confers increased tolerance to a wide range of abiotic stresses. J Plant Physiol 164:1626–1638
• Lowry OH, Rosebrough NT, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–275
• Mantri NL, Ford R, Coram TE, Pang ECK (2007) Transcriptional profiling of chickpea genes differentially regulated in response to high-salinity, cold and drought. BMC Genomics 8:303
• Mantri NL, Ford R, Coram TE, Pang ECK (2010) Evidence of unique and shared responses to major biotic and abiotic stresses in chickpea. Environ Exp Bot 69:286–292
• Millan T, Clarke HJ, Siddique KHM, Buhariwalla HK, Gaur M, Kumar J, Gill J, Kahl G, Winter P (2006) Chickpea molecular breeding: new tools and concepts. Euphytica 147:81–103
• Morsey MR, Jouve L, Husman JF, Hoffmann L, Stewart JM (2007) Alteration of oxidative and carbohydrate metabolism under abiotic stress in two rice (Oryza sativa L.) genotypes contrasting in chilling tolerance. J Plant Physiol 164:157–167
• Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acid Res 8:4321–4325
• Nergiz C, Gokgoz E (2007) Effects of traditional cooking methods on some antinutrients and in vitro protein digestibility of dry bean varieties (Phaseolus vulgaris L.) grown in turkey. Int J Food Sci Technol 42:868–873
• Oktem HA, Eyidooan F, Demirba DAT, Bayrac OZ, Ozygur MTE, Selcuk F, Yucel M (2008) Antioxidant responses of lentil to cold and drought stress. J Plant Biochem Biotech 17:15–21
• Parida AK, Das AB (2004) Effects of NaCl stress on nitrogen and phosphorus metabolism in true mangrove Bruguiera parviflora grown under hydroponic culture. J Plant Physiol 161:921–928
• Prasad TK, Anderson MD, Martin BA, Stewart CR (1994) Evidence for chilling induced oxidative stress in maize seedlings and a regulating role for hydrogen peroxidase. Plant Cell 6:65–74
• Rajjou L, Debeaujon I (2008) Seed longevity: survival and maintenance of high germination ability of dry seeds. CR Biologies 331:796–805
• Ramakrishna V, Jhansi RP, Ramakrishna RP (2006) Anti-nutritional factors during germination in Indian bean (Dolichos lablab L.) seeds. World J Dairy Food Sci 1:06–11
• Rohlf FJ (1998) NTSYS-pc. Numerical taxonomy and multivariate analysis system, version 2.0. Exeter Software, Setauket
• Rouser G, Fleisher S, Yamamoto A (1974) Two dimensional thin layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots. Lipids 5:494–496
• Ryan J (1997) A global perspectives on pigeonpea and chickpea sustainable production systems: present status and future potential. In recent advances in pulses research. In: Asthana A, Ali M (eds) Indian Society for Pulses Research and Development, pp 1–31
• Scandalios JG (1993) Oxygen stress and superoxide dismutases. Plant Physiol 101:7–12
• Seki M, Narusaka M, Ishida J, Nanjo T, Umezawa T, Kamiya T, Nakajiima M, Kawai J (2002) Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high salinity stresses using a full length cDNA library. Plant J 31:279–292
• Sinha AK (1971) Colorimetric assay of catalase. Anal Biochem 47:389–394
• Tang W, Newton RJ (2006) Polyamines reduce salt-induced oxidative damage by increasing the activities of antioxidant enzymes by increasing the activities of antioxidative enzymes and decreasing lipid peroxidation in Virginia pine. Plant Growth Regul 46:31–43
• Wahid A, Perveen M, Gelani S, Basra SMA (2007) Pretreatment of seed with H₂O₂ improves salt tolerance of wheat seedlings by alleviation of oxidative damage and expression of stress proteins. J Plant Physiol 164:283–294
• Wang YC, Qu GZ, Li HY, Wu YJ, Wang C, Liu GF, Yang CP (2010) Enhanced salt tolerance of transgenic poplar plants expressing a manganese superoxide dismutase from Tamarix androssowii. Mol Biol Rep 37:1119–1124
• Wold S, Esbensen E, Geladi P (1987) Principle component analysis. Chemom Intell Lab Syst 3:37–52
• Xing YU, Jia W, Zhang J (2007) AtMEK1 mediates stress-induced gene expression of CAT1 catalase by triggering H₂O₂ production in Arabidopsis. J Exp Bot 58:2969–2981
• Yasmin A, Zeb A, Khalil AW, Paracha GM, Khattak AB (2008) Effect of processing on antinutritional factors of red kidney bean (Phaseolus vulgaris). Food Bioprocess Technol 1:415–419
• Zemel MB, Shelef LA (1982) Phytic acid hydrolysis with soluble zinc and iron in whole wheat bread as affected by calcium containing additives. J Food Science 47:535–537

Uwagi

Rekord w opracowaniu