Plasma proteomic profiling of African catfish (Clarias gariepinus)

• Autorzy: Herosimczyk A. , Ozgo M. , Lepczynski A. , Ciechanowicz A. , Colussi A. , Seniuta P. , Skrzypczak W. F.
• Języki publikacji: EN

Abstrakty

EN

This study was aimed at profiling plasma proteins of healthy 8-month-old African catfish. Plasma proteins within the isoelectric point ranging from 3.0 to 10.0 were separated by high resolution two-dimensional electrophoresis (2-DE). In total, 8 different gene products were identified with a matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometer. These included carrier proteins (fatty acid-binding protein 1, metallothionein, mitoferrin-2), a structural protein (vimentin), proteins involved in the regulation of transcription (thyroid hormone receptor alpha A, endothelial differentiation-related factor 1 homolog), and others (B-cell lymphoma 6 protein homolog, FGFR1 oncogene partner 2 homolog). This is the first study attempting to map the plasma proteome of Clarias gariepinus. Owing to the lack of information concerning catfish protein sequences in the relevant databases, protein spots were identified by matching peptide data to interspecies homology. The results of this preliminary study may encourage other authors to undertake further research on the plasma proteome of the African catfish.

Słowa kluczowe

EN

blood plasma; proteomics; African catfish; Clarias gariepinus; fish; plasma protein

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2013
• Tom: 69
• Numer: 10
• Opis fizyczny: p.612-615,fig.,ref.

Twórcy

autor

Herosimczyk A.

• Department of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Doktora Judyma 6, 71-466 Szczecin, Poland

autor

Ozgo M.

• Department of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Doktora Judyma 6, 71-466 Szczecin, Poland

autor

Lepczynski A.

• Department of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Doktora Judyma 6, 71-466 Szczecin, Poland

autor

Ciechanowicz A.

• Department of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Doktora Judyma 6, 71-466 Szczecin, Poland

autor

Colussi A.

• Department of Biology, Faculty of Natural Sciences and Mathematics, Swiss Federal Institute of Technology ETH Zurich, Wolfgang-Pauli Str. 27, CH-8093 Zurich, Switzerland

autor

Seniuta P.

• Department of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Doktora Judyma 6, 71-466 Szczecin, Poland

autor

Skrzypczak W. F.

• Department of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Doktora Judyma 6, 71-466 Szczecin, Poland

Bibliografia

• 1.Appelbaum S., McGeer J. C.: Effect of diet and light regime on growth and survival of African catfish (Clarias gariepinus) larvae and early juveniles. Aquacult. Nutr. 1998, 3, 157-164.
• 2.Booth N. J., Bilodeau-Bourgeois A.: Proteomic analysis of head kidney tissue from high and low susceptibility families of channel catfish following challenge with Edwardsiella ictaluri. Fish Shellfish Immun. 2009, 26, 193-196.
• 3.Brunt J., Hansen R., Jamieson D. J., Austin B.: Proteomic analysis of rainbow trout (Oncorhynchus mykiss, Walbaum) serum after administration of probiotics in diets. Vet. Immunol. Immunopathol. 2008, 121, 199-205.
• 4.Dowd W. W., Renshaw G. M. C., Cech J. J., Kültz D.: Compensatory proteome adjustments imply tissue-specific structural and metabolic reorganization following episodic hypoxia or anoxia in the epaulette shark (Hemiscyllium ocellatum). Physiol. Genomics 2010, 42, 93-114.
• 5.Herosimczyk A., Dejeans N., Sayd T., O¿go M., Skrzypczak W. F., Mazur A.: Plasma proteome analysis: 2D gels and chips. J. Physiol. Pharmacol. 2006, 57 (Supp. 7), 81-93.
• 6.Herrmann H., Münick M. D., Brettel M., Fouquet B., Markl J.: Vimentin in a cold-water fish, the rainbow trout: highly conserved primary structure but unique assembly properties. J. Cell Sci. 1996, 109, 569-578.
• 7.Isani G., Andreani G., Carpenè E., Di Molfetta S., Eletto D., Spisni E.: Effects of waterborne Cu exposure in gilthead sea bream (Sparus aurata): A proteomic approach. Fish Shellfish Immun. 2011, 31, 1051-1058.
• 8.Mohanty B. P., Bhattacharjee S., Das M. K.: Lens proteome map and α-crystallin profile of the catfish Rita rita. Indian J. Biochem. Biophys. 2011, 48, 35-41.
• 9.Nelson E. R., Habibi H. R.: Thyroid receptor subtypes: structure and function in fish. Gen. Comp. Endocr. 2009, 161, 90-96.
• 10.Osman A. G. M., Al-Awadhi R. M., Harabawy A. S. A., Mahmoud U. M.: Evaluation of the use of protein electrophoresis of the African catfish Clarias gariepinus (Burchell, 1822) for biomonitoring aquatic pollution. Environ. Res. J. 2010, 4, 235-243.
• 11.Pierrard M. A., Kestemont P., Phuong N. T., Tran M. P., Delaive E., Thezenas M. L., Dieu M., Raes M., Silvestre F.: Proteomic analysis of blood cells in fish exposed to chemotherapeutics: Evidence for long term effects. J. Proteomics 2012, 75, 2454-2467.
• 12.Polak-Juszczak L.: Chemical characteristics of fishes new to the Polish market. Acta Sci. Pol., Piscaria 2007, 6, 23-32.
• 13.Russell S., Hayes M. A., Simko E., Lumsden J. S.: Plasma proteomic analysis of the acute phase response of rainbow trout (Oncorhynchus mykiss) to intraperitoneal inflammation and LPS injection. Dev. Comp. Immunol. 2006, 30, 393-406.
• 14.Scudiero R., Temussi P. A., Parisid E.: Fish and mammalian metallothioneins: a comparative study. Gene 2005, 345, 21-26.
• 15.Shaw G. C., Cope J. J., Li L., Corson K., Hersey C., Ackermann G. E., Gwynn B., Lambert A. J., Wingert R. A., Traver D., Trede N. S., Barut B. A., Zhou Y., Minet E., Donovan A., Brownlie A., Balzan R., Weiss M. J., Peters L. L., Kaplan J., Zon L. I., Paw B. H.: Mitoferrin is essential for erythroid iron assimilation. Nature 2006, 440, 96-100.
• 16.Smet H. de, Blust R., Moens L.: Absence of albumin in the plasma of the common carp Cyprinus carpio: binding of fatty acids to high density lipoprotein. Fish Physiol. Biochem. 1998, 19, 71-81.
• 17.Veiseth-Kent E., Grove H., Færgestad E. M., Fjæra S. O.: Changes in muscle and blood plasma proteomes of Atlantic salmon (Salmo salar) induced by crowding. Aquaculture 2010, 309, 272-279.