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2018 | 27 | 2 |

Tytuł artykułu

Optimizing toluene degradation by bacterial strain isolated from oil-polluted soils

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The presence of toxic compounds like toluene has caused extensive contamination in oil-contaminated environments. Using bacteria to degrade monoaromatic compounds could be a good approach to finding a suitable bioaugmentation agent. In this study on toluene, degrading bacterial species were isolated from oil-contaminated environments (located in Bandar-Anzali, Guilan, Iran). The strain has been molecularly identified as Bacillus cereus ATHH39 (Accession number: KX344721) by partial sequencing of the 16S rDNA gene. Response surface methodology (RSM) was used for biodegradation of toluene by ATHH39 by implementing the central composite design (CCD). The central composite design (CCD) was applied to optimize and investigate pH, temperature, and toluene concentrations and their interactions for enhancing cell growth and toluene degradation by ATHH39 under in vitro conditions. The variables (pH, temperature, and toluene concentrations) with the highest significant impacts on growth and toluene degradation were selected. According to the prediction and optimization function of the design expert software, the optimum conditions of cell growth and toluene degradation were found. When pH, temperature, and toluene concentration were adjusted to 6.72, 33.16ºC and 824.15 mg/l, respectively, cell growth and toluene degradation reached OD₆₀₀ = 0.69 and 64.11%, respectively, which is very close to the predicted cell growth and toluene degradation of OD₆₀₀ = 0.71 and 65.85%, indicating that the response surface methodology optimization of process parameters for cell growth and toluene degradation is reliable. Based on the results, the ATHH39 strain was introduced as a useful microorganism with the potential for bioremediation of wastewater containing toluene.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

27

Numer

2

Opis fizyczny

p. 655-663,fig.,ref.

Twórcy

  • Soil Science Department, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
autor
  • Soil Science Department, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
  • Department of Microbiology, Rasht Branch, Islamic Azad University, Rasht, Iran
  • Basic Medical Sciences Department, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
autor
  • Department of Chemistry, Rasht Branch, Islamic Azad University, Rasht, Iran

Bibliografia

  • 1. Ortega -Gonzalez D.K., Zaragoza D., Aguirre -Garrido J., Ramírez -Saad H., Hernández -Rodríguez C., Jan -Roblero J. Degradation of benzene, toluene, and xylene isomers by a bacterial consortium obtained from rhizosphere soil of Cyperus sp. grown in a petroleum-contaminated area. Folia Microbiologica. 58 (6), 569, 2013.
  • 2. Mazzeo D.E., Levy C.E., De Angelis D.D., Marin-Morales M.A. BTEX biodegradation by bacteria from effluents of petroleum refinery. Science of the Total Environment. 408 (20), 4334, 2010.
  • 3. El-Naas M.H., Acio J.A., El Telib A.E. Aerobic biodegradation of BTEX: Progresses and Prospects. Journal of Environmental Chemical Engineering. 2 (2), 1104, 2014.
  • 4. Munoz R., Diaz L.F., Bordel S., Villaverde S. Inhibitory effects of catechol accumulation on benzene biodegradation in Pseudomonas putida F1 cultures. Chemosphere. 68 (2), 244, 2007.
  • 5. Song Y., Jiang B., Tian S., Tang H., Liu Z., Li C., Jia J., Huang W.E., Zhang X., Li G. A whole-cell bioreporter approach for the genotoxicity assessment of bioavailability of toxic compounds in contaminated soil in China. Environmental Pollution. 195, 178, 2014.
  • 6. Zhang L., Zhang C., Cheng Z., Yao Y., Chen J. Biodegradation of benzene, toluene, ethylbenzene, and o-xylene by the bacterium Mycobacterium cosmeticum byf-4. Chemosphere. 90 (4), 1340, 2013.
  • 7. Iheanacho C.C., Okerentugba P.O., Orji F.A., Ataikiru T.L. Hydrocarbon degradation potentials of indigeneous fungal isolates from a petroleum hydrocarbon contaminated soil in Sakpenwa community, Niger Delta. Global Advanced Research Journal of Environmental Science and Toxicology. 3 (1), 6, 2014.
  • 8. Ali K., Alennabi A. Study on biodegradation of Miri and Masila crude oil and used car oil by microorganisms isolated from Malaysian soil and the effect of aeration and NPK addition on biodegradation process. Dissertation, for Doctoral Degree. Malaysia: University Pahang, Malaysia. 2012.
  • 9. Mishra S., Singh S.N., Pande V. Bacteria induced degradation of fluoranthene in minimal salt medium mediated by catabolic enzymes in vitro condition. Bioresource Technology. 164, 299, 2014.
  • 10. Khleifat K.M. Biodegradation of phenol by Ewingella Americana: effect of carbon starvation and some growth conditions. Process Biochemistry. 41 (9), 2010, 2006.
  • 11. Shourian M., Noghabi K.A., Zahiri H.S., Bagheri T., Karballaei G., Mollaei M., Rad I., Ahadi S., Raheb J., Abbasi H. Efficient phenol degradation by a newly characterized Pseudomonas sp. SA01 isolated from pharmaceutical Wastewaters. Desalination. 246 (1), 577, 2009.
  • 12. Myers R.H., Montgomery D.C., Anderson-Cook C.M. Response Surface Methodology: process and product optimization using designed experiments, 4th ed.; John Wiley and Sons, 856, 2016.
  • 13. Zhang Z., Zheng H. Optimization for decolorization of azo dye acid green 20 by ultrasound and H₂O₂ using response surface methodology. Journal of Hazardous Materials. 172 (2), 1388, 2009.
  • 14. Rani Usha M., Rastogi N.K., Appaiah K.A. Statistical optimization of medium composition for bacterial cellulose production by Gluconacetobacter hanenii UAC09 using Coffee Cherry Husk extract-an agro-industry waste. Journal of Microbiology and Biotechnology. 21, 739, 2011.
  • 15. Lee S.H., Lee W.S., Lee C.H., Kim J.G. Degradation of phenanthrene and pyrene in rhizosphere of grasses and legumes. Journal of Hazardous Materials. 153 (1), 892, 2008.
  • 16. Carter G.R., John R., Cole J.R. Diagnostic procedure in veterinary bacteriology and mycology, Academic Press, 2012.
  • 17. Raieta K., Muccillo L., Colantuoni V. A novel reliable method of DNA extraction from olive oil suitable for molecular traceability. Food Chemistry. 172, 596, 2015.
  • 18. McPherson M.J., Muller S.G. PCR. The basic from background to bench, 1st ed.; Bios scientific publishers, Oxford, New York, 2000.
  • 19. Madueno L., Coppotelli B.M., Alvarez H.M., Morelli I.S. Isolation and characterization of indigenous soil bacteria for bioaugmentation of PAH contaminated soil of semiarid Patagonia, Argentina. International Biodeterioration and Biodegradation. 65 (2), 345, 2011.
  • 20. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution. 28 (10), 2731, 2011.
  • 21. Malatova K. Isolation and characterization of hydrocarbon degrading bacteria from environmental habitats in western New York State, 2005.
  • 22. Wang L., Qiao N., Sun F., Shao Z. Isolation, gene detection and solvent tolerance of benzene, toluene and xylene degrading bacteria from nearshore surface water and Pacific Ocean sediment. Extremophiles. 12 (3), 335, 2008.
  • 23. Guven G., Perendeci A.,Tanyolaç A. Electrochemical treatment of deproteinated whey wastewater and optimization of treatment conditions with response surface methodology. Journal of hazardous materials. 157 (1), 69, 2008.
  • 24. Said K.A., Amin M.A. Overview on the Response Surface Methodology (RSM) in Extraction Processes. Journal of Applied Science & Process Engineering. 2 (1), 8, 2016.
  • 25. Azaman S.N., Ramakrishn an N.R., Tan J.S., Rahim R.A., Abdullah M.P., Ariff A.B. Optimization of an induction strategy for improving interferon-α2b production in the periplasm of Escherichia coli using response surface methodology. Biotechnology and Applied Biochemistry. 56 (4), 141, 2010.
  • 26. Aghaie E., Pazouki M., Hosseini M.R., Ranjbar M., Ghavipanjeh F. Response surface methodology (RSM) analysis of organic acid production for Kaolin beneficiation by Aspergillus Niger. Chemical Engineering Journal. 147 (2), 245, 2009.
  • 27. Ramanan R.N., Tan J.S., Mohamed M.S., Ling T.C., Tey B.T., Ariff A.B. Optimization of osmotic shock process variables for enhancement of the release of periplasmic interferon-α2b from Escherichia coli using response surface method. Process Biochemistry. 45 (2), 196, 2010.
  • 28. Singh R., Celin S.M. Biodegradation of BTEX (benzene, toluene, ethyl benzene and xylene) compounds by bacterial strain under aerobic conditions. Journal of Ecobiotechnology. 2 (4), 27, 2010.
  • 29. Prabhakaran P., Sureshbabu A., Rajakumar S., Ayyasamy P.M. Bioremediation of crude oil in synthetic mineral salts medium enriched with aerobic bacterial consortium. International Journal of Innovative Research in Science, Engineering and Technology. 3 (2), 9236, 2014.
  • 30. Alagappan G., Cowan R.M. Effect of temperature and dissolved oxygen on the growth kinetics of Pseudomonas putida F1 growing on benzene and toluene. Chemosphere. 54 (8), 1255, 2004.
  • 31. Hamed T.A., Bayraktar E., Mehmetoglu U., Mehmetoglu T. The biodegradation of benzene, toluene and phenol in a two-phase system. Biochemical Engineering Journal. 19 (2), 137, 2004.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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