Systematic overestimation of Salicaceae seed survival using radicle emergence in response to drying and storage: implications for ex situ seed banking

• Autorzy: Popova E.V. , Kim D. H. , Han S. H. , Moltchanova E. , Pritchard H. W. , Hong Y. P.
• Języki publikacji: EN

Abstrakty

EN

Biodiversity conservation programmes are underpinned by seed banking following drying to low water contents (WC), and supported by both the assessment and prediction of seed viability over time. The means of judging viability is thus crucial to the comprehension of seed vigour. We selected seeds of three species and one hybrid in the Salicaceae likely to have variation in tolerance to drying, processing and storage, including in relation to cryobanking, and compared survival growth as radicle emergence (germination) and normal seedling production. With three seed lots of Salix gracilistyla, air-drying to 8–10 % WC enhanced seed survival after 40 days’ storage at 5 °C as compared with non-treated seeds at 14–20 % WC. Four seed lots of Populus alba × P. glandulosa showed equally high germination (88–100 %) and proportions of normal seedlings (81–99 %) when stored at 5 °C for 7–10 weeks. Among seven seed lots of S. gracilistyla, two groups with different storage behaviour could be statistically distinguished with normal seedling production ranging from 0 to 45 % after storage at 5 °C for 13 weeks. Seed tolerance to WC manipulation and cryopreservation was very variable among species and seed lots. Seed lots of S. hallaisanensis and S. gracilistyla with ~80 % germination survived cryopreservation at 10 % WC, but were sensitive to lower WCs. In contrast, Populus seeds had greater desiccation tolerance combined with cryopreservation capability. With seed lots of all species and hybrids, cryopreservation had little effect on viability unless the high moisture freezing limit had been exceeded (~10–20 % WC, depending on seed lot). However, under all conditions of handling (drying, rehydration, storage at 5 °C or cryopreservation) using germination as the only indicator of viability over-estimated survival compared with normal seedling production.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 10
• Opis fizyczny: p.3015-3025,fig.,ref.

Twórcy

autor

Popova E.V.

• Division of Forest Genetic Resources, Korea Forest Research Institute, 44-3 Omokdong, Suwon 441-350, Korea

autor

Kim D. H.

• Division of Forest Genetic Resources, Korea Forest Research Institute, 44-3 Omokdong, Suwon 441-350, Korea

autor

Han S. H.

• Division of Forest Genetic Resources, Korea Forest Research Institute, 44-3 Omokdong, Suwon 441-350, Korea

autor

Moltchanova E.

• Department of Mathematics and Statistics, University of Canterbury, Christchurch 8140, New Zealand

autor

Pritchard H. W.

• Seed Conservation Department, Royal Botanic Gardens, Kew, Wakehurst Place, Ardingly, West Sussex RH17 6TN, UK

autor

Hong Y. P.

• Division of Forest Genetic Resources, Korea Forest Research Institute, 44-3 Omokdong, Suwon 441-350, Korea

Bibliografia

• Bonner F (1986) Technologies to maintain tree germplasm diversity. In: Technologies to maintain biological diversity, V. 2. Office of technology and assessment, Washington, DC, pp 630–672
• Chmielarz P (2009) Cryopreservation of dormant European ash (Fraxinus excelsior) orthodox seeds. Tree Physiol 29:1279–1285
• Chmielarz P (2010) Cryopreservation of the non-dormant orthodox seeds of Ulmus glabra. Acta Biol Hungarica 61:224–233
• Daws MI, Pritchard HW (2008) The development and limits of freezing tolerance in Acer pseudoplatanus fruits across Europe is dependent on provenance. CryoLetters 29:189–198
• Densmore R, Zasada J (1983) Seed dispersal and dormancy patterns on northern willows: ecological and evolutionary significance. Can J Bot 61:3207–3216
• Dussert S, Engelmann F (2006) New determinants for tolerance of coffee (Coffea arabica L.) seeds to liquid nitrogen exposure. CryoLetters 27:169–178
• Gosling P (2007) Forestry Commission practice guide. Forestry Commission, Edinburgh
• Häggman H, Rusanen M, Jokipii S (2008) Cryopreservation of in vitro tissues of deciduous forest trees. In: Reed BM (ed) Plant Cryopreservation: a practical guide. Springer, New York, pp 365–386
• Han SH, Kim DH, Lee JC (2010) Cadmium and zinc interaction and phytoremediation potential of seven Salix caprea clones. J Ecol Field Biol 33:245–251
• Hinchee MAW, Mullinax LN, Rottmann W (2010) Wood biomass and purpose-grown trees as teedstocks for renewable energy. In: Mascia PN, Scheffran J, Thomas SR, Widholm JM (eds) Biotechnology in agriculture and forestry, vol 66., Plant biotechnology for sustainable production of energy and coproductsSpringer, Berlin, pp 155–208
• International Seed Testing Association (1999) International Rules for Seed Testing. Seed Sci Technol (Supplement) 27:333
• Kim HH, Lee JH, Shin DJ, Ko HC, Hwang HS, Kim TS, Cho EG, Engelmann F (2008) Desiccation sensitivity and cryopreservation of Korean ginseng seeds. CryoLetters 29:419–426
• Maroder HL, Prego IA, Facciuto GR, Maldonado SB (2000) Storage behavior of Salix alba and Salix matsudana seeds. Ann Bot 86:1017–1021
• Melchior GH (1985) Genetic differences in ability of aspen families to sustain long term storage of seeds. In: Nather J (ed) Proceedings of the international symposium on seed problems under stressful conditions. Vienna and Gmunden, Austria, pp 58–70
• Pence VC (1996) Germination, desiccation and cryopreservation of seed of Populus deltoides. Barr Seed Sci Technol 24:151–157
• Pohjonen V (2008) Energy forests with Salix as a carbon dioxide sink. http://velipohjonen.wordpress.com/article/energy-forests-withsalix-as-a-carbon-29gytge2im5s4-4. Assessed on 09 June 2013
• Popova EV, Kim DH, Han SH, Pritchard HW, Lee JC (2012) Narrowing of the critical hydration window for cryopreservation of Salix caprea seeds following ageing and a reduction in vigour. CryoLetters 33:219–230
• Pritchard HW (2007) Cryopreservation of desiccation-tolerant seeds. In: Day JG, Stacey GN (eds) Methods in molecular biology, vol 368., Cryopreservation and freeze-drying protocols. Humana Press Inc., Totowa, pp 185–201
• Rossi G, Parolo G, Zonta LA, Crawford JA, Leonardi A (2006) Salix herbacea L. fragmented small population in the N-Apennines (Italy): response to human trampling disturbance. Biodiver Conserv 15:3881–3893
• Royal Botanic Gardens Kew (2008) Seed Information Database (SID). Version 7.1. http://data.kew.org/sid/. Assessed on 11 May 2013
• Sato Y (1955) On the viability of Salicaceae seeds. Res Bull College Exp Forests Hokkaido Univ 17:225–266
• Schreiner EJ (1974) Populus L. In: Seeds of woody plants in the United States. Agriculture handbook No 450. Washington DC, Forest Service, USDA, pp 645–655
• Simak M (19802) Germination and storage of Salix caprea L. and Populus tremula L. seeds. In: Wang BSP, Pitel JA (eds) Proceedings of the IUFRO international symposium on forest tree seed storage Petawawa/Canada. Environment Canada, Canada Forestry Service, Canada, pp 142–160
• Tauer CG (1979) Seed tree, vacuum, and temperature effects on eastern cottonwood seed viability during extended storage. Forest Sci 25:112–114
• Walters C, Wheeler L, Stanwood PC (2004) Longevity of cryogenically stored seeds. Cryobiology 48:229–244
• Walters CT, Hill LM, Volk GM, Haiby K (2011) ‘‘Intermediate’’ seed storage physiology: Populus as a natural model system. In: Meeting Abstract. International Society for Seed Science, Brazil 10–15 Apr 2011, pp 280
• Wang BSP (19802) Long-term storage of Abies, Betula, Larix, Piceas, Pinus and Populus seeds. In: Wang BSP, Pitel JA (eds) Proceedings of the IUFRO international symposium on forest tree seed storage, Petawawa/Canada. Environment Canada, Canada Forestry Service, Canada, pp 212–218
• Wood CB, Pritchard HW, Lindegaard K (2003) Seed cryopreservation and longevity of two Salix hybrids. CryoLetters 24:17–26
• Zasada JC, Densmore R (1977) Changes in Salicaceae seed viability during storage. Seed Sci Technol 5:509–517

Uwagi

rekord w opracowaniu