EN
Root-lesion nematodes, Pratylenchus spp., are economically important, plant-damaging parasitic nematodes occurring in a wide variety of crops. The most common species is Pratylenchus neglectus which is found on cereal crops, especially on wheat. The proper identification of this nematode species is crucial because of significant morphological similarity to another Pratylenchus species. There are some diagnostic methods described in publication. The aim of this work was to test previously described protocol and its usefulness for Polish populations of this pathogen. Moreover, the new specific PCR protocols were described. These methods can be used both, for identification of Polish population of P. neglectus, and on the basis of the amplified rDNA region for the genetic correlation and phylogenetic studies.