Proliferation and apoptosis in the rabbit ovary after administration of T-2 toxin and quercetin

• Autorzy: Lesniak-Walentyn A. , Kolesarova A. , Medvedova M. , Maruniakova N. , Capcarova M. , Kalafova A. , Hrabia A. , Sirotkin A. V.
• Języki publikacji: EN

Abstrakty

EN

T-2 mycotoxin, a natural contaminant of cereals and agricultural products, is known to exhibit the severest toxicity and the lowest LD50 value among trichothecenes. Since quercetin has been reported to have strong antioxidant properties, the effect of quercetin and T-2 toxin on rabbit ovarian cell proliferation and apoptosis was assessed. In the ovaries, proliferating cells were analysed immunocytochemically (proliferating cell nuclear antigen as a marker) and apoptotic cells by the terminal deoxynucleotidyl transferase (TdT)-mediated biotinylated deoxyuridine triphosphates (dUTP) nick endlabeling (TUNEL) method. It was found that: 1. a single dose of T-2 toxin (0.08 mg · kg−1 body weight (BW) 72 h before slaughter) decreased cell proliferation, 2. chronic exposure to quercetin (1 mg · kg−1 BW; 90 days, 3 times per week) results in increased cell proliferation and reduced cell apoptosis, 3. quercetin has the potential to attenuate T-2 toxin-induced proliferation arrest. To clarify the mechanism of T-2 toxin and quercetin action in the rabbit ovary, further experiments are necessary.

Słowa kluczowe

EN

proliferation; apoptosis; rabbit; ovary; T-2 toxin; quercetin

• Wydawca: -
• Czasopismo: Journal of Animal and Feed Sciences
• Rocznik: 2013
• Tom: 22
• Numer: 3
• Opis fizyczny: p.264-271,fig.,ref.

Twórcy

autor

Lesniak-Walentyn A.

• Department of Animal Physiology and Endocrinology, University of Agriculture in Krakow, Al.Mickiewicza 24/28, 30-059 Krakow, Poland

autor

Kolesarova A.

• Department of Animal Physiology, Slovak University of Agriculture in Nitra, Tr.A.Hlinku 2,949 76 Nitra, Slovak Republic

autor

Medvedova M.

• Department of Animal Physiology, Slovak University of Agriculture in Nitra, Tr.A.Hlinku 2,949 76 Nitra, Slovak Republic

autor

Maruniakova N.

• Department of Animal Physiology, Slovak University of Agriculture in Nitra, Tr.A.Hlinku 2,949 76 Nitra, Slovak Republic

autor

Capcarova M.

• Department of Animal Physiology, Slovak University of Agriculture in Nitra, Tr.A.Hlinku 2,949 76 Nitra, Slovak Republic

autor

Kalafova A.

• Department of Animal Physiology, Slovak University of Agriculture in Nitra, Tr.A.Hlinku 2,949 76 Nitra, Slovak Republic

autor

Hrabia A.

• Department of Animal Physiology and Endocrinology, University of Agriculture in Krakow, Al.Mickiewicza 24/28, 30-059 Krakow, Poland

autor

Sirotkin A. V.

• Institute of Genetics and Reproduction of Farm Animals, Animal Production Research Centre Nitra, Hlohovecka 2, 949 92 Luzianky, Slovak Republic

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