AFLP-profiling of long-term stored and regenerated rye genebank samples

• Autorzy: Chwedorzewska K.J. , Bednarek P. T. , Puchalski J. , Krajewski P.
• Języki publikacji: EN

Abstrakty

EN

The aim of these studies was to analyse the genetic changes induced by natural aging during long-term seed storage of rye. For this purpose, the AFLP (Amplified Fragment Length Polymorphism) technique was applied. In the experiment, DNA variation was demonstrated in seven-day-old seedlings of four seed samples of cv. Dankowskie Zlote, showing different levels of viability following long-term storage. Among the 362 AFLP fragments analysed, 73 had significantly different frequencies in at least one of the series. Principle Coordinate Analysis (PCA) based on molecular data revealed differences between the progenies of naturally aged seed samples with variable initial viability. It was clearly shown that materials with low viability differed in structure from highly viable ones, and that the population changes exhibited in the first case are preserved through regenerations. Although changes that were observed for initially viable samples were not so significant, they still occurred - probably as a result of genetic shift.

Słowa kluczowe

EN

AFLP method; rye; Secale cereale; gene bank; sample; seed aging; genetic change; storage; long-term storage

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2002
• Tom: 07
• Numer: 2A
• Opis fizyczny: p.457-463,fig.,ref.

Twórcy

autor

Chwedorzewska K.J.

• The Botanical Garden – the Centre for the Conservation of Biological Diversity, Polish Academy of Sciences, Prawdziwka 2, 02-973 Warsaw, Poland

autor

Bednarek P. T.

• The Botanical Garden – the Centre for the Conservation of Biological Diversity, Polish Academy of Sciences, Prawdziwka 2, 02-973 Warsaw, Poland

autor

Puchalski J.

• The Botanical Garden – the Centre for the Conservation of Biological Diversity, Polish Academy of Sciences, Prawdziwka 2, 02-973 Warsaw, Poland

autor

Krajewski P.

• Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, Poznan, Poland

Bibliografia

• 1. Roos, E. E. Physiological, biochemical, and genetical changes in seed quality during storage. Hort. Sci. 15 (1980) 781-784.
• 2. Osborne, D. J., Sharon, R. and Ben-Ishai, R. Studies on DNA integrity and DNA repair in germinating embryos of rye (Secale cereale). Israel J; Bot. 29 (1980/81) 259-272.
• 3. Murata, M., Tsuchiya, T. and Roos, E. E. Chromosome damage induced by artificial seed aging in barley 3. Theor. AppL. Genet. 67 (1984) 161-170.
• 4. Abdalla, F. H. and Roberts, E. H. The effects of temperature moisture on the genetic changes in seeds of barley, broad beans, and peas during storage. Ann. Bot. 33 (1969) 153-167.
• 5. Dourado, A. M. and Roberts, E. H. The nature of the virido-albina/ striata mutant induced during storage in the barley seeds. Ann. Bot. 54 (1984) 791-798.
• 6. Roos, E. E. Genetic shifts in mixed bean populations. I. Storage effects. Crop Sci. 24 (1984) 240-244.
• 7. Roos, E. E. Genetic shifts in mixed bean populations. II. Effects of regeneration. Crop Sci. 24 (1984) 711-715.
• 8. Bednarek, P. T., Chwedorzewska, K. and Puchalski. J. Preliminary molecular studies on genetic changes in rye seeds due to long - term storage and regeneration. In: Challenges In Rye Germplasm Conservation International Plant Genetic Resources Institute, Rome, 1998, 54-61.
• 9. Shatters, R. G. Jr., Schweder, M. E., West, S. H., Abdelghany, A. and Smith, R. L. Environmentally induced polymorphisms detected by RAPD analysis of soybean seed DNA. Seed Sci. Res. 5 (1995) 106-116.
• 10. Roos, E. E. and Rincker, C. M. Genetic stability in 'Pennlate' orchardgrass seed following artificial ageing. Crop Sci. 22 (1988) 611-613.
• 11. Stoyanova, S. D. Genetic shift and variations of gliadins induced by seed ageing. Seed Sci. Teclmol. 19 (1991) 363-371.
• 12. Stoyanova, S. D. Effects of seed ageing and regeneration on the genetic composition of wheat. Seed Sci. Technol. 20 (1992) 489-496.
• 13. Marcos-Filho, J. and McDonald, M. B. Sensitivity of RAPD analysis, germination and vigour tests to detect the intensity of deterioration of naturally and artificially aged soybean seeds. Seed Sci. Technol. 26 (1998) 141-156.
• 14. Vos, P., Hogers, R., Bleeker, M., Lee, T. van, Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M. and Zauber, M. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 23 (1995) 4407-4414.
• 15. Bednarek, P. T., Chwedorzewska, K., Króliczak, J., Puchalski, J. and Zawada, M. AFLP molecular markers-as a tool for genetic variability studies of rye inbred lines. Biul. Inst. Hod. Aklim. Rośl. 211 (1999) 219-227.
• 16. Mardia, K. V., Kent, J. T. and Bibby, J. M. Multivariate analysis. Academic Press, London. (1979).
• 17. Niedzielski, M. and Puchalski, J. Observation of chosen morphological traits in rye accessions in relation to long-term seed storage and regeneration. In: Challenges in Rye Germplasm Conservation, International Plant Genetic Resources Institute, Rome, 1998, 45-49.
• 18. Puchalski, J., Izoenzymy jako markery zmian genetycznych w siewkach żyta wywołanych przechowywaniem i regeneracją. Prace Ogrodu Botanicznego PAN. Monografie i rozprawy 4 (1993) 86.