Simvastatin attenuates intestinal ischaemia/reperfusion - induced injury in rat

• Autorzy: Hajipour B. , Somi M. H. , Saberifar F. , Hemmati M. R. , Asl N. A. , Moein A. , Vatankhah A. M. , Nourazar A. R. , Nasirizade M. R.
• Języki publikacji: EN

Abstrakty

EN

Ischaemia/reperfusion (I/R) injury is commonly seen in the field of intestine surgical interventions, shock, trauma, and many other clinical conditions. Simvastatin is known to have antioxidant and anti-inflammatory properties. This study investigated the effect of simvastatin administration in a warm intestinal I/R model on TNF-α, antioxidant enzymes and intestinal tissue morphology. Thirty-six male wistar rats underwent laparotomy under general anaesthesia. Simvastatin was administered from four days before ischaemia induction. The rats were divided in to three groups (n = 12): the sham goup, the I/R group, and the I/R + simvastatin group. Intestinal ischaemia was induced by superior mesenteric artery ligation with microvascular clamps for 60 minutes, and after ischaemia, blood perfusion was released into the tissue and a reperfusion phase was started, which lasted for 3 hours. After 3 hours, the animals were sacrificed and serum and tissue obtained for biochemical and histological study. In the simvastatin treated group, intestinal tissue injury, TNF-α level, and tissue malondealdehyde levels were significantly lower than in the I/R group (p < 0.05). Glutathion peroxidase and superoxide dismutase levels were significantly higher in the simvastatin treated group than in the I/R group (p < 0.05). Simvastatin pretreatment reduced intestinal I/R injury and was associated with down- -regulation of serum TNF-α and tissue malondealdehyde level, and simvastatin administration maintained cellular antioxidant enzyme contents compared to the I/R group after 3 hours reperfusion time. (Folia Morphol 2009; 68, 3: 156–162)

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2009
• Tom: 68
• Numer: 3
• Opis fizyczny: p.156-162,fig.,ref.

Twórcy

autor

Hajipour B.

• Faculty of Medicine, Islamic Azad University, Tabriz Branch, PostBox: 51385-3633, Tabriz, Iran

autor

Somi M. H.

• Liver and Gastroenterology Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran

autor

Saberifar F.

• Scientific Association, Faculty of Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran

autor

Hemmati M. R.

• Department of Pathology, Faculty of Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran

autor

Asl N. A.

• Department of Physiology, Tabriz University of Medical Sciences, Tabriz, Iran

autor

Moein A.

• Scientific Association, Faculty of Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran

autor

Vatankhah A. M.

• Drug Applied Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran

autor

Nourazar A. R.

• Departement of Physiology, Faculty of Veterinary, Islamic Azad University, Tabriz Branch, Tabriz, Iran

autor

Nasirizade M. R.

• Departement of Physiology, Faculty of Veterinary, Islamic Azad University, Tabriz Branch, Tabriz, Iran

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