Molecular detection of metallo-beta-lactamase and putative virulence genes in environmental isolates of Pseudomonas species

• Autorzy: Igbinosa I.H. , Igbinosa E. O. , Okoh A. I.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to investigate the presence of metallo-β-lactamase (Imp-1, Vim-1, Vim-2) -resistant genes and putative virulence genes (ExoS, Apr, LasB, PhzM) in some environmental isolates of Pseudomonas species recovered from freshwater, wastewater, cultivated soil, plant root, and plant rhizosphere samples. Polymerase chain reaction (PCR) was employed to evaluate the presence and established metallo-β- lactamase and virulence genes using specific primer sets. About 6.7% of the Pseudomonas isolates from wastewater treatment plant were found to harbour the cytotoxin gene, while 20%, 20.6%, and 6.7% of the isolates were positive for alkaline protease, phenazine and Vim-2 genes, respectively. For the Pseudomonas species isolated from plant rhizosphere, 14.3% were positive for phenazine gene, while 10.7% were positive for Vim-2 gene. Dissemination of the target genes were in the following pattern: all isolates that harboured ExoS, Apr, and Vim-2 genes from wastewater sample were found to be Pseudomonas putida, while phenazine gene was found in 75% of Pseudomonas putida isolates and 25% of other Pseudomonas species. All plant rhizosphere isolates harbouring phenazine and Vim-2 genes were observed to be Pseudomonas putida species. The incidence of Vim-2 and virulence genes in some environmental isolates of Pseudomonas species suggest that these isolates are reservoirs of metallo-β-lactamase-resistant genes and are potential pathogens that may be of serious public health significance.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2014
• Tom: 23
• Numer: 6
• Opis fizyczny: p.2327-2331,ref.

Twórcy

autor

Igbinosa I.H.

• Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare Private Bag X1314, Alice 5700 South Africa

autor

Igbinosa E. O.

• Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare Private Bag X1314, Alice 5700 South Africa

autor

Okoh A. I.

• Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare Private Bag X1314, Alice 5700 South Africa

Bibliografia

• 1. WALSH F., ROGERS T.R. Detection of blaVim_2 carbapene- mase in Pseudomonas aeruginosa in Ireland. J. Antimicrob. Chemoth. 61, 219, 2008.
• 2. BEBRONE C. Metallo-ß-lactamases (classification, activi­ty, genetic organization, structure, zinc coordination) and their superfamily. Biochem. Pharmacol. 74, 1686, 2007.
• 3. SACHA P., WIECZOREK P., HAUSCHILD T., ZORAWS- KI M., OLSZAŃSKA D., TRYNISZEWSKA E. Metallo-ß- lactamases of Pseudomonas aeruginosa - a novel mecha­nism resistance to ß-lactam antibiotics. Folia Histochem. Cyto. 46, 137, 2008.
• 4. LIBISCH B., WATINE J., BALOGH B., GACS M., MUZSLAY M., SZABÓ G., FÜZI M. Molecular typing indicates an important role for two international clonal complexes in dissemination of Vim -producing Pseudomonas aeruginosa clinical isolates in Hungary. Res. Microbiol. 159, 162, 2008.
• 5. SANTOS C., CAETANO T., FERREIRA S., MENDO S. Tn5090-like class 1 integron carrying blayim-2 in a Pseudomonas putida strain from Portugal. Clin. Microbiol. Infec. 16, 1558, 2010.
• 6. WALSH T.R., TOLEMAN M.A., POIREL L., NORD­MANN P. Metallo-ß-lactamases: the quiet before the storm? Clin. Microbiol. Rev. 18, 306, 2005.
• 7. FINNAN S., MORRISSEY J.P., O'GARA F., BOYD E.F. Genome diversity of Pseudomonas aeruginosa isolates from cystic fibrosis patients and the hospital environment. J. Clin. Microbiol., 42, 5783, 2004.
• 8. HAMOOD A.N., GRISWOLD J., COLMER J. Characterization of elastase - deficient clinical isolates of Pseudomonas aeruginosa. Infect. Immun. 64, 3154, 1996.
• 9. MCIVER K.S., OLSON J.C., OHMAN D.E. Pseudomonas aeruginosa lasB1 mutants produce an elastase, substituted at active-site His-223 that is defective in activity, processing, and secretion. J. Bacteriol. 175, 4008, 1993.
• 10. ALLEWELT M., COLEMAN F.T., GROUT M., PRIEBE G.P., PIER G.B. Acquisition of expression of the Pseudomonas aeruginosaExoUcytotoxin leads to increased bacterial virulence in a murine model of acute pneumonia and systemic spread. Infect. Immun. 68, 3998, 2000.
• 11. KURAHASHI K., KAJIKAWA O., SAWA T., OHARA M., GROPPER M. A., FRANK D.W., MARTIN T.R., WIENER-KRONISH J.P. Pathogenesis of septic shock in Pseudomonas aeruginosa pneumonia. J. Clin. Invest. 104, 743, 1999.
• 12. SMIRNOV V.V., KIPRIANOVA E.A., GARAGULIA A.D., DODATKO T.A. Piliashenko II. Antibiotic activity and siderophores of Pseudomonas cepacia. Prikl. Biokhim. Mikrobiol. 26, 75, 1990.
• 13. MAVRODI D.V., BONSALL R.F., DELANEY S.M., SOULE MJ., PHILLIPS G., THOMASHOW L.S. Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aerugi­nosa PAO1. J. Bacteriol. 183, 6454, 2001.
• 14. IGBINOSA I.H., NWODO U.U., SOSA A., TOM M., OKOH A.I. Commensal Pseudomonas species isolated from waste- water and freshwater milieus in the Eastern Cape Province, South Africa, as reservoir of antibiotic resistant determinants Int'l. J. Environ. Res. Public Health. 9, 2537, 2012.
• 15. IGBINOSA I.H., TOM M., OKOH A.I. Antibiogram char­acteristics and associated resistance genes of commensal Pseudomonas species isolated from soil and plant rhizos­phere in the Eastern Cape Province, South Africa. J. Pure Appl Microbiol. 6, 1541, 2012.
• 16. POIREL L., NAAS T., NICOLAS D., COLLET L., BEL­LAIS S., CAVALLO J.D., NORDMANN P. Characterization of Vim-2, a carbapenem- hydrolyzing met- allo-ß-lactamase and its plasmid - and integronborne gene from a Pseudomonas aeruginosa clinical isolate in France. Antimicrob. Agents Ch. 44, 891, 2000.
• 17. KOH T.H., WANG G.C.Y., SNG L. IMP-1 and a novel met- allo-ß-lactamase, Vim-6, in fluorescent pseudomonads iso­lated in Singapore. Antimicrob. Agents Ch. 48, 2334, 2004.
• 18. SIARKOU V.I., VITTI D., PROTONOTARIOU E., IKONOMIDIS A., SOFIANOU D. Molecular epidemiolo­gy of outbreak-related Pseudomonas aeruginosa strains car­rying the novel variant blaVim-l7 metallo-ß-lactamase gene. Antimicrob. Agents Ch. 53, 1325, 2009.
• 19. QUINTEIRA S., FERREIRA H., PEIXE L. First isolation of blaVim-2 in an environmental isolate of Pseudomonas pseudoalcaligenes. Antimicrob. Agents Ch. 49, 2140, 2005.
• 20. CHOY M.H., STAPLETON F., WILLCOX M.D.P., ZHU H. Comparison of virulence factors in Pseudomonas aerug- inosa strains isolated from contact lens- and non-contact lens-related keratitis. J. Med. Microbiol. 57, 1539, 2008.
• 21. TINGPEJ P., SMITH L., ROSE B., ZHU H., CONIBEAR T., AL NASSAFI K., MANOS J., ELKINS M., BYE P., WILLCOX M., BELL S., WAINWRIGHT C., HARBOUR C. Phenotypic characterization of clonal and nonclonal Pseudomonas aeruginosa strains isolated from lungs of adults with cystic fibrosis. J. Clin. Microbiol. 45, 1697, 2007.
• 22. SADIKOT R.T., BLACKWELL T.S., CHRISTMAN J.W., PRINCE A.S. Pathogen-host interactions in Pseudomonas aeruginosa pneumonia. Am. J. Resp. Crit. Care 171, 1209, 2005.
• 23. POIREL L., CABANNE L., COLLET L., NORDMANN P. Class II transposonborne structure harboring metallo-ß-lac- tamase gene blaVim-2 in Pseudomonas putida. Antimicrob. Agents Ch. 50, 2889, 2006.
• 24. CORVEC S., POIREL L., ESPAZE E., GIRAUDEAU C., DRUGEON H., NORDMANN P. Long-term evolution of a nosocomial outbreak of Pseudomonas aeruginosa produc­ing Vim-2 metallo-enzyme. Hosp. Infect. 68, 73, 2008.
• 25. Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial disk susceptibility tests. Approved Standard, 9th edn. Wayne, PA; 2006.
• 26. ALMUZARA M., RADICE M., GARATE N.D., KOSS- MAN A., CUIROLO A., SANTELLA G., FAMIGLIETTI A., GUTKIND G. VIM-2-producing Pseudomonas putida, Buenos Aires. Emerg. Infect. Dis. 13, 668, 2007.
• 27. BOGAERTS P., HUANG T-D., RODRIGUEZ-VILLALO­BOS H., BAURAING C., DEPLANO A., STRUELENS M.J., GLUPCZYNSKI Y. Nosocomial infections caused by multidrug-resistant Pseudomonas putida isolates producing Vim-2 and Vim-4 metallo-ß-lactamases. J. Antimicrob. Chemoth. 61, 749, 2008.
• 28. HORII T., MURAMATSU H., IINUMA Y. Mechanisms of resistance to fluoroquinolones and carbapenems in Pseudomonas putida. J. Antimicrob. Chemoth. 56, 643, 2005.