Prevalence of subclinical endometritis in repeat breeding cows and mRNA expression of tumor necrosis factor α and inducible nitric oxide synthase in the endometrium of repeat breeding cows with and without subclinical endometritis

• Autorzy: Janowski T. , Baranski W. , Lukasik K. , Skarzynski D. , Rudowska M. , Zdunczyk S.
• Języki publikacji: EN

Abstrakty

EN

Information on the prevalence of subclinical endometritis and its mechanism in repeat breeding cows is very limited. The aims of this study were: a) to evaluate the incidence of this disorder with cytobrush cytology b) to analyze mRNA expression of tumor necrosis factor α (TNFα) and inducible nitric oxide synthase (iNOS) in endometrial biopsy samples collected from repeat breeding cows with and without subclinical endometritis. Two experiments were carried out. In experiment 1, 112 (12.4%) repeat breeding cows (inseminated at least 3 times and not pregnant) were selected out of 902 cows from 8 dairy herds. Cytobrush cytology was performed on these cows, using the threshold of 10% PMNs in uterine smears. The results showed that 45 out of the 112 cows (40.2%) were diagnosed as having subclinical endometritis. In experiment 2, uterine biopsy samples were taken from repeat breeding cows with subclinical endometritis (n = 10) and without this disorder (n = 10). Using reverse transcription-PCR, the mRNA expression of TNFα and iNOS was determined. A statistically significant increase in expression of both substances was measured in the group of cows with subclinical endometritis (p < 0.05). These results provide evidence for a high prevalence of subclinical endometritis in repeat breeding cows as well as the involvement of TNFα and iNOS pathways in the regulation of this pathological condition.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2013
• Tom: 16
• Numer: 4
• Opis fizyczny: p.693-699,fig.,ref.

Twórcy

autor

Janowski T.

• Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-719 Olsztyn, Poland

autor

Baranski W.

• Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-719 Olsztyn, Poland

autor

Lukasik K.

• Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research of Polish Academy of Science, Bydgoska 7, 10-243 Olsztyn, Poland

autor

Skarzynski D.

• Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research of Polish Academy of Science, Bydgoska 7, 10-243 Olsztyn, Poland

autor

Rudowska M.

• Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-719 Olsztyn, Poland

autor

Zdunczyk S.

• Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-719 Olsztyn, Poland

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