Pathogenicity variation and mycelia compatibility groups in Sclerotina sclerotiorum

• Autorzy: Irani H. , Heydari A. , Javan-Nikkhah M. , Ibrahimov A. S.
• Języki publikacji: EN

Abstrakty

EN

Population variability of S. sclerotiorum, the causal agent of Sclerotinia stalk rot of sunflower, was determined by mycelial compatibility grouping (MCG) and pathogenicity variation comparison. To study mycelial compatibility grouping and pathogenicity variability, isolates of S. sclerotiorum were collected from sunflower fields in East, West Azerbaijan and Ardebil provinces of Iran. Among 186 isolates tested, 26 MCGs were identified and 46% were represented by single isolates. There were differences among MCGs comparing mycelial growth rate, sclerotial production on PDA and aggressiveness cause disease. Significant differences were detected in number of sclerotia, dry weight of sclerotia, mycelial growth rate and aggressiveness among MCGs (p < 0.001) regardless of their geographic origins. There was generally a poor correlation (r = 0.21, p ≤ 0.05) between sclerotia weight and number of sclerotia produced on PDA and also to the mycelial growth rate at 24 (r = 0.35, p ≤ 0.05) and 48h (r = 0.39, p ≤ 0.05). Our studies in comparison of the detached leaf and cut-stem methods showed that the highest rank correlations (r = 0.78 p ≤ 0.01), while aggressiveness of two inoculation methods (stem and leaf detached) were not correlated to colony diameter growth or the other two factors. Variation in isolates aggressiveness may be important considerations in disease management systems.

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2011
• Tom: 51
• Numer: 4
• Opis fizyczny: p.329-336,fig.,fot.,ref.

Twórcy

autor

Irani H.

• Department of Biology, University of Baku, Republic of Azerbaijan

autor

Heydari A.

autor

Javan-Nikkhah M.

autor

Ibrahimov A. S.

Bibliografia

• Alvarez E., Molina M.L. 2000. Characterizing the Sphaceloma fungus, causal agent of super-elongation disease in Cassava. Plant Dis. 84 (4): 423-428.
• Anderson J.B., Kohn L.M. 1995. Clonality in soilborne, plant pathogenic fungi. Ann. Rev. Phytopathol. 33 (1): 369-391.
• Atallah Z.K., Larget B., Chen X., Johnson D.A. 2004. High genetic diversity, phenotypic uniformity, and evidence of outcrossing in Sclerotinia sclerotiorum in the Columbia basin of Washington state. Phytopathology 94 (7): 737-742.
• Auclair J., Boland G.J., Kohn L.M., Rajcan I. 2004. Genetic interactions between Glycine max and Sclerotinia sclerotiorum using a straw inoculation method. Plant Dis. 88 (8): 891-895.
• Boland G. J., Hall R. 1994. Index of plant hosts of Sclerotinia sclerotiorum. Can. J. Plant Pathol. 16 (2): 93-108.
• Carbone I., Anderson J.B., Kohn L.M. 1999. Patterns of descent in clonal lineages and their multilocus fingerprints are resolved with combined gene genealogies. Evolution 53: 11-21.
• Carbone I., Kohn L.M. 2001. Multilocus nested haplotype networks extended with DNA fingerprints show common origin and fine-scale, ongoing genetic divergence in a wild microbial metapopulation. Mol. Ecol. 10 (10): 2409-2422.
• Carpenter M.A., Frampton C., Stewart A. 1999. Genetic variation in New Zealand populations of the plant pathogen Sclerotinia sclerotiorum. New Zealand J. Crop. Hortic. Sci. 27: 13-21
• Cubeta M., Cody B., Kohli Y., Kohn L.M. 1997. Clonality in S. sclerotiorum on infected cabbage in eastern North Carolina. Phytopathology 87 (10): 1000-1004.
• Durman S.B., Menendez A.B., Godeas A.M. 2001. Mycelial compatibility groups in Sclerotinia sclerotiorum from agricultural fields in Argentina. p. 27-28. In: Proc. XI Int. Sclerotinia Workshop (C. Young, K. Hughes, eds.). York, UK, 8-12 July 2001, 193 pp.
• Durman S.B., Menendez A.B., Godeas A.M. 2003. Mycelial compatibility groups in Buenos Aires field populations of Sclerotinia sclerotiorum (Sclerotiniaceae). Aust. J. Bot. 51 (3): 421-427.
• Garrabrandt L.E., Johnston S.A., Peterson J.L. 1983. Tan sclerotia of Sclerotinia sclerotiorum from lettuce. Mycologia 75 (3): 451-456.
• Glass N.L., Kaneko I. 2003. Fatal attraction: Nonself recognition and heterokaryon incompatibility in filamentous fungi. Euk. Cell. 2 (1): 1-8.
• González M., Rodríguez R., Zavala M.E., Jacobo J.L., Hernández F., Acosta J. 1998. Characterization of Mexican isolates of Colletotrichum lindemuthianum by using differential cultivars and molecular markers. Phytopathology 88 (4): 292-299.
• Hambleton S., Walker C., Kohn L.M. 2002. Clonal lineages of Sclerotinia sclerotiorum previously known from other crops predominate in 1999-2000 samples from Ontario and Quebec soybean. Can. J. Plant Pathol. 24 (3): 309-315.
• Hind T.L., Ash G.J., Murray G.M. 2003. Prevalence of sclerotinia stem rot of canola in New South Wales. Aust. J. Exp. Agric. 43 (2): 1-6.
• Irani H., Ershad J., Alizadeh A. 2001. The effect of soil depth, moisture and temperature on sclerotium germination of Sclerotinia sclerotiorum and its pathogenicity. Iranian J. Plant Path. 37 (3-4): 185-195.
• Kohn L.M., Carbone I., Anderson J.B. 1990. Mycelial interactions in Sclerotinia sclerotioum. Exp. Mycol. 14 (2): 255-267.
• Kohn L.M., Stasovski E., Carbone I., Royer J., Anderson J.B. 1991. Mycelial incompatibility and molecular markers identify genetic variability in field populations of Sclerotinia sclerotiorum. Phytopathology 81 (2): 480-485.
• Kohn L.M. 1995. The clonal dynamic in wild and agricultural plant pathogen populations. Can. J. Bot. 73 (Suppl. 1): 1231-1240.
• Kohli Y., Morrall R.A.A., Anderson J.B., Kohn L.M. 1992. Local and trans-Canadian clonal distribution of Sclerotinia sclerotiorum on canola. Phytopathology 82: 875-880.
• Kohli Y., Brunner L.J., Yoell H., Kohn L.M. 1995. Clonal dispersal and spatial mixing in populations of the plant pathogenic fungus, Sclerotinia sclerotiorum. Mol. Ecol. 4 (10): 69-77.
• Kull L.S., Vuong T.D., Powers K.S., Eskridge K.M., Steadman J.R., Hartman G.L. 2003. Evaluation of three resistance screening methods using six Sclerotinia sclerotiorum isolates and three entries of each soybean and dry bean. Plant Dis. 87: 1471-1476.
• Kull L.S., Pedersen W.L., Palmquist D., Hartman G.L. 2004. Mycelial compatibility grouping and aggressiveness of Sclerotinia sclerotiorum. Plant Dis. 88 (4): 325-332.
• Li G.Q., Huang H.C., Laroche A., Acharaya S.N. 2003. Occurrence and characterization of hypovirulence in the tan sclerotial isolates of S10 of Sclerotinia sclerotiorum. Mycol. Res. 107 (11): 1350-1360.
• Li Z.Q. 2004. (Chinese, with English abstract). Sunflower diseases and control in Inner Mongolia. Inner Mongolia. Agric. Sci. Tech. 6: 63-64.
• Li Z., Zhan M., Wang Y., Li R., Fernando W.G.D. 2008. Mycelial compatibility group and pathogenicity variation of Sclerotinia sclerotioum populations in sunflower from China, Canada and England. Plant Pathol. J. 2 (2): 131-139.
• Marukawa S., Funalawa S., Satomura Y. 1975. Some physical and chemical factors on formation of sclerotia in Sclerotinia libertiana Fucle. Aric. Biol. Chem. 39: 463-468.
• Morrall R., Deczek J., Sheard J. 1972. Variation and correlation within and between morphology, pathogencity, and pectolytic enzyme activity in Sclerotinia from Saskatchewan. Can. J. Bot. 50 (4): 767-786.
• Price K.C., Colhoun J. 1975. A study of variability of isolates of Sclerotinia sclerotiorum (Lib.) de Bary from different hosts. Phytopathology 83: 159-166.
• Sirjusingh C., Kohn L.M. 2001. Characterization of microsatellites in the fungal plant pathogen, Sclerotinia sclerotiorum. Mol. Ecol. Notes. 1 (4): 267-269.
• Schafer M. R., Kohn L.M. 2006. An optimized method for mycelial compatibility testing in Sclerotinia sclerotiorum. Mycologia 98 (4): 593-597.
• Sprague S., Stewart-Wade S. 2002. Sclerotinia in canola - results from petal and disease surveys across Victoria in 2001. In: Grains research and development corporation research update-southern region, Australia. Grains Research and Development Corporation, Victoria, 78 pp.
• Zhao J., Peltier A. J., Meng J., Osborn T.C., Grau C. R. 2004. Evaluation of Sclerotinia stem rot resistance in oilseed Brassica napus using a petiole inoculation technique under greenhouse conditions. Plant Dis. 88 (9): 1033-1039.

Uwagi

Rekord w opracowaniu