Identification of Heterobasidion spp.in Poland by RFLP analysis of laccase and manganese dependent peroxidase

• Autorzy: Kraj W. , Kowalski T.
• Języki publikacji: EN

Abstrakty

EN

Laccase and manganese dependent peroxidase (MnP) genes in H. annosum s.l. were studied by PCR-RFLP. The peroxidase genes MNP1a and MNP2 showed Heterobasidion species specific length differences. Among eighteen monomorphic markers which were foundfor investigatedgenes, three were characteristic for H. abietinum andfive for H. annosum s.s. The remaining specific markers were characteristic for H. parviporum and H. abietinum (nine markers) or for H. parviporum and H. annosum s.s. (one marker). No specific marker for H. parviporum was detected. On the basis of monomorphic markers, intersterility groups of eighty one strains isolatedfrom eleven forest tree or bush species in south Polandwere identified. Fifty-three belongedto H. annosum s.s., twenty-five to H. parviporum, andthree to H. abietinum. Strains belonging to H. annosum s.s. were isolatedfrom Pinus nigra, P. strobus, P. sylvestris, Larix decidua, Picea abies, as well as from Alnus incana, Betula pendula, Padus avium, Quercus robur and Q. rubra. H. parviporum was isolatedfrom Abies alba, Picea abies, Pinus nigra, P. sylvestris, Larix decidua andfrom Alnus incana. This is the first report of H. parviporum occurrence on Alnus incana in Poland. The ascertained H. abietinum strains derived from Abies alba. Our results demonstrate a new methodof Heterobasidion species identification based on the different length of MNP1a and MNP2 peroxidase genes and RFLP markers obtained for laccase and Mn2+ dependent peroxidase genes.

• Wydawca: -
• Czasopismo: Dendrobiology
• Rocznik: 2010
• Tom: 63
• Opis fizyczny: p.11-19,fig.,ref.

Twórcy

autor

Kraj W.

• Department of Forestry Pathology, Agricultural University, Al.9Listopada 46, 31-425 Krakow, Poland

autor

Kowalski T.

Bibliografia

• Asiegbu F.O., Johansson M., Woodward S., Hüttermann A. 1998. Biochemistry of the host-parasite interaction. In: Heterobasidion annosum. Biology, ecology, impact andcontrol. WoodwardS., StenlidJ., Karjalainen R., Hüttermann A. (eds.). CAB International, Wallingford, pp. 167–193.
• Asiegbu F.O., Abu S., StenlidJ., Johansson M. 2004. Sequence polymorphism andmolecular characterization of laccase genes of the conifer pathogen Heterobasidion annosum. Mycological Research 108: 136–148.
• Asiegbu F.O., Adomas A., Stenlid J. 2005. Conifer root andbutt rot causedby Heterobasidion annosum (Fr.) Bref. s.l. Molecular Plant Pathology 6: 395–409.
• Capretti P., Korhonen K., Mugnai L., Romagnoli C. 1990. An intersterility group of Heterobasidion annosum specializedto Abies alba. European Journal of Forest Pathology 20: 231–240.
• Carlson J.E., Tulsieram L.K., Glaubitz J.C., Luk V.W.K., Kauffeldt C., Rutledge R. 1991. Segregation of random amplified DNA markers in F1 progeny of conifers. Theoretical and Applied Genetics 83: 194–200.
• Chase T.E., Otrosina W.J., Spieth P.T., Cobb F.W. 1991. Use of PCR to distinguish biological species within the Heterobasidion annosum complex. Phytopathology (Abstr.) 81: 1190.
• Dai Y.C., Vainio E.J., Hantula J., Niemelä T., Korhonen K. 2003. Investigations on Heterobasidion annosum s.lat. in central andeastern Asia with the aidof mating tests and DNA fingerprinting. Forest Pathology 33: 269–286.
• Daniel G., Asiegbu F., Johansson M. 1998. The saprotrophic wood-degrading abilities of Heterobasidium annosum intersterility groups P andS. Mycological Research 102: 991–997.
• Domański S. 1976. Fungi occurring in forests injured by air pollutants in the Upper Silesia and Cracow industrial regions of Poland. IV. Higher fungi causing root diseases within forest stands not rebuilt in the years 1971–1975. Acta Agraria et Silvestria, Series Silvestris 16: 61–73.
• Fabritius A.L., Karjalainen R. 1993. Variation in Heterobasidion annosum detected by random amplified polymorphic DNAs. European Journal of Forest Pathology 23: 193–200.
• Garbelotto M., Bruns T.D., Cobb F.W., Otrosina W.J. 1993. Differentiation of intersterility groups and geographic provenances among isolates of Heterobasidion annosum detected by random amplified polymorphic DNA assays. Canadian Journal of Botany 71: 565–569.
• Garbelotto M., Otrosina W.J., Cobb F.W., Bruns T.D. 1998. The European S andF intersterility groups of Heterobasidion annosum may represent sympatric protospecies. Canadian Journal of Botany 76: 397–409.
• Gonthier P., Garbelotto M., Varese G.C., Nicolotti G. 2001. Relative abundance and potential dispersal range of intersterility groups of Heterobasidion annosum in pure andmixedforests. Canadian Journal of Botany 79: 1057–1065.
• Haars A., Chet I., Hüttermann A. 1981. Effect of phenolic compounds and tannin on growth and laccase activity of Fomes annosus. European Journal of Forest Pathology 11: 67–76.
• Harrington T.C., Rizz D.M. 1999. Defining species in the fungi. In: Structure andd ynamics of fungal populations. Worrall J.J. (ed.). Kluwer Press, The Netherlands, Dordrecht, pp. 43–71.
• Johannesson H., StenlidJ. 2003. Molecular markers reveal genetic isolation andphylogeography of the S and F intersterility groups of the wood-decay fungus Heterobasidion annosum. Molecular Phylogenetics and Evolution 29: 94–101.
• Johansson M. 1988. Pectic enzyme activity of spruce (S) andpine (P) strains of Heterobasidion annosum (Fr.) Bref. Physiological andMolecular Plant Pathology 33: 333–349.
• Johansson M., Denekamp M., Asiegbu F.O. 1999. Production and isozyme pattern of extracellular laccase in the S andP intersterility groups of the root pathogen Heterobasidion annosum. Mycological Research 103: 365–371.
• Korhonen K. 1978. Intersterility groups of Heterobasidion annosum. Metsãntutkimuslaitoksen Julkaisuja. Communicationes Instituti Forestalis Fenniae 94: 1–25.
• Kowalski T., Łakomy P. 1998. A new recordof Heterobasidion annosum (Fr.) Bref. F group occurrence in Polandin connection with interesting mycological findings. Phytopathologica Polonica 15: 49–55.
• La Porta N., Capretti P., Kammiovirta K., Karjalainen R., Korhonen K. 1997. Geographical cline of DNA variation within the F intersterility group of Heterobasidion annosum in Italy. Plant Pathology 46: 773–784.
• Łakomy P. 1996. F group of Heterobasidion annosum foundin Poland. European Journal of Forest Pathology 26, 217–222.
• Łakomy P., Kowalski T., Werner A. 2000. Preliminary report on distribution of Heterobasidion annosum intersterility groups in Poland. Acta Mycologica 35: 303–309.
• Łakomy P., Werner A. 2003. Distribution of Heterobasidion annosum intersterility groups in Poland. Forest Pathology 33: 105–112.
• Maijala P., Harrington T.C., Raudaskoski M. 2003. A peroxidase gene family and gene trees in Heterobasidion and related genera. Mycologia 95: 209–221.
• Niemelä T., Korhonen K. 1998. Taxonomy of the genus Heterobasidion. In: Heterobasidion annosum. Biology, ecology, impact andcontrol. WoodwardS., StenlidJ., Karjalainen R., Hüttermann A. (eds.). CAB International, Wallingford, pp. 27–33.
• Otrosina W.J., Chase T.E., Cobb F.W., Korhonen K. 1993. Population structure of Heterobasidion annosum from North America andEurope. Canadian Journal of Botany 71: 1064–1071.
• Schulze S., Bahnweg G. 1998. Critical review of identification techniques for Armillaria spp. and Heterobasidion annosum root andbutt rot diseases. Journal of Phytopathology 146: 61–72.
• Sierota Z. 1998. Fungal diseases in last years in Poland. Proceedings from the IUFRO WP 7.03.10 Workshop. Ustroń-Jaszowiec (Poland), April 21–24, 1998. Methodology of Forest Insect and Disease Survey in Central Europe, 153–155.
• StenlidJ., Karlsson J.O. 1991. Partial intersterility in Heterobasidion annosum. Mycological Research 95: 1153–1159.
• Vainio E.J., Hantula J. 1999. Variation of RAMS markers within the intersterility groups of Heterobasidion annosum in Europe. European Journal of Forest Pathology 29: 231–246.
• Werner A., Łakomy P. 2002. Intraspecific variation in Heterobasidion annosum for mortality rate on Pinus sylvestris and Picea abies seedlings grown in pure culture. Mycologia 94: 856–861.
• Żółciak A. 1992. Intersterile groups of Heterobasidion annosum (Fr.) Bref. – identification of Polish isolates. Prace Instytutu Badawczego Leśnictwa 741: 83–90.

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