Expression and regulation of a pear polygalacturonase inhibitor protein gene (PpPGIP1) during fruit development, under salicylic acid treatment, and in diseased fruit

• Autorzy: Shi H-Y , Zhang Y.-X. , Chen L.
• Języki publikacji: EN

Abstrakty

EN

To elucidate the roles of genes during fruit development, a fruit-predominant cDNA (designated PpPGIP1) encoding a polygalacturonase inhibitor protein (PGIP) was isolated from a pear (Pyrus pyrifolia Nakai. cv. Whangkeumbae) fruit cDNA library. The PpPGIP1 gene shares high sequence homology at the nucleotide level in the coding region and at the amino acid level with other plant PGIP genes. Subsequently, the corresponding PpPGIP1 gene was isolated from pear genomic DNA. Real-time quantitative PCR analyses revealed that PpPGIP1 transcripts were functionally accumulated during the developing fruit of pear. The PpPGIP1 expression signals were detected at relatively low level in early stage of pear fruit development (30–140 days after full bloom). With the fruit ripening, softening and senescence, the expression of PpPGIP1 gene increased to a relative high level in 150–180 days after full bloom fruit. In addition, PpPGIP1 gene was dramatically up-regulated in 180 days after full bloom diseased fruit and by 0.2 mM salicylic acid (SA) treatment for 12 h in 150 days after full bloom fruit. These results suggested that PpPGIP1 might play an important role in response to disease resistance and SA during fruit development of pear.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 11
• Opis fizyczny: p.3181-3189,fig.,ref.

Twórcy

autor

Shi H-Y

• College of Horticulture, Agricultural University of Hebei, Baoding 071001, China

autor

Zhang Y.-X.

• College of Horticulture, Agricultural University of Hebei, Baoding 071001, China

autor

Chen L.

• Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China

Bibliografia

• Cao H, Bowling SA, Gordon AS, Dong X (1994) Characterization of an Arabidopsis mutant that is nonresponsive to inducers of systemic acquired resistance. Plant Cell 6:1583–1592
• Clark SE, Williams RW, Meyerowitz EM (1997) The CLAVATA1 gene encodes a putative receptor kinase that controls shoot and floral meristem size in Arabidopsis. Cell 89:575–585. doi:10.1016/S0092-8674(00)80239-1
• Di Matteo A, Federici L, Mattei B, Salvi G, Johnson KA, Savino C (2003) The crystal structure of polygalacturonase-inhibiting protein (PGIP), a leucine-rich repeat protein involved in plant defense. Proc Natl Acad Sci USA 100:10124–10128. doi:10.1073/pnas.1733690100
• Favaron F, D’Ovidio R, Porceddu E, Alghisi P (1994) Purification and molecular characterization of a soybean polygalacturonaseinhibiting protein. Planta 195:80–87. doi:10.1007/BF00206295
• Ferrari S, Vairo D, Ausubel FM, Cervone F, De Lorenzo G (2003) Tandemly duplicated Arabidopsis genes that encode polygalacturonase-inhibiting proteins are regulated coordinately by different signal transduction pathways in response to fungal infection. Plant Cell 15:93–106. doi:10.1105/tpc.005165
• Ferrari S, Galletti R, Vairo D, Cervone F, De Lorenzo G (2006) Antisense expression of the Arabidopsis thaliana AtPGIP1 gene reduces polygalacturonase-inhibiting protein accumulation and enhances susceptibility to Botrytis cinerea. Mol Plant Microbe Interact 19:931–936. doi:10.1094/MPMI-19-0931
• Gunes A, Inal A, Alpaslan M, Eraslan F, Bagci EG, Cicek N (2007) Salicylic acid induced changes on some physiological parameters symptomatic for oxidative stress and mineral nutrition in maize (Zea mays L.) grown under salinity. J Plant Physiol 164:728–736. doi:10.1016/j.jplph.2005.12.009
• Johnston DJ, Ramanathan V, Williamson B (1993) A protein from immature raspberry fruits which inhibits endopolygalacturonases from Botrytis cinerea and other micro-organisms. J Exp Bot 44:971–976. doi:10.1093/jxb/44.5.971
• Jones JD (2001) Putting knowledge of plant disease resistance genes to work. Curr Opin Plant Biol 4:281–287. doi:10.1016/S1369-5266(00)00174-6
• Lambert C, Leonard N, De Bolle X, Depiereux E (2002) ESyPred3D: prediction of proteins 3D structures. Bioinformatics 18:1250–1256. doi:10.1093/bioinformatics/18.9.1250
• Leckie F, Mattei B, Capodicasa C, Hemmings A, Nuss L, Aracri B (1999) The specificity of polygalacturonase-inhibiting protein (PGIP): a single amino acid substitution in the solvent-exposed beta-strand/beta-turn region of the leucine-rich repeats (LRRs) confers a new recognition capability. EMBO J 18:2352–2363. doi:10.1093/emboj/18.9.2352
• Li J, Chory J (1997) A putative leucine-rich repeat receptor kinase involved in brassinosteroid signal transduction. Cell 90:929–938. doi:10.1016/S0092-8674(00)80357-8
• Li R, Rimmer R, Yu M, Sharpe AG, Seguin-Swartz G, Lydiate D (2003) Two Brassica napus polygalacturonase inhibitory protein genes are expressed at different levels in response to biotic and abiotic stresses. Planta 217:299–308
• Mattei B, Bernalda MS, Federici L, Roepstorff P, Cervone F, Boffi A (2001) Secondary structure and post-translational modifications of the leucine-rich repeat protein PGIP (polygalacturonaseinhibiting protein) from Phaseolus vulgaris. Biochemistry 40:569–576. doi:10.1021/bi0017632
• Montoya T, Nomura T, Farrar K, Kaneta T, Yokota T, Bishop GJ (2002) Cloning the tomato Curl3 gene highlights the putative dual role of the leucine-rich repeat receptor kinase tBRI1/SR160 in plant steroid hormone and peptide hormone signaling. Plant Cell 14:3163–3176. doi:10.1105/tpc.006379
• Oliveira MB, Nascimento LB, Junior ML, Petrofeza S (2010) Characterization of the dry bean polygalacturonase-inhibiting protein (PGIP) gene family during Sclerotinia sclerotiorum (Sclerotiniaceae) infection. Genet Mol Res 9:994–1004. doi:10.4238/vol9-2gmr776
• Penninckx IAMA, Eggermont K, Terras FRG, Thomma BPHJ, Samblanx GWD, Buchala A (1996) Pathogen-induced systemic activation of a plant defensin gene in Arabidopsis follows a salicylic acid-independent pathway. Plant Cell 8:2309–2323
• Ramanathan V, Simpson CG, Thow G, Iannetta PPM, McNicol RJ, Williamson B (1997) cDNA cloning and expression of polygalacturonase inhibiting proteins (PGIPs) from red raspberry (Rubus idaeus). J Exp Bot 48:1185–1193. doi:10.1093/jxb/48.6.1185
• Rivas-San Vicente M, Plasencia J (2011) Salicylic acid beyond defence: its role in plant growth and development. J Exp Bot 62:3321–3338. doi:10.1093/jxb/err031
• Shi HY, Zhang YX (2012) Pear ACO genes encoding putative 1-aminocyclopropane-1-carboxylate oxidase homologs are functionally expressed during fruit ripening and involved in response to salicylic acid. Mol Biol Rep 39:9509–9519. doi:10.1007/s11033-012-1815-5
• Shi HY, Zhu L, Zhou Y, Li G, Chen L, Li XB (2009) A cotton gene encoding a polygalacturonase inhibitor-like protein is specifically expressed in petals. Acta Biochim Biophys Sin 41:316–324. doi:10.1093/abbs/gmp020
• Srivastava MK, Dwivedi UN (2000) Delayed ripening of banana fruit by salicylic acid. Plant Sci 158:87–96
• Stotz HU, Powell ALT, Damon SE, Greve LC, Bennett AB, Labavitch JM (1993) Molecular characterization of a polygalacturonase inhibitor from Pyrus communis L. cv. Bartlett. Plant Physiol 102:133–138. doi:10.1104/pp.102.1.133
• Stotz HU, Contos JJA, Powell ALT, Bennett AB, Labavitch JM (1994) Structure and expression of an inhibitor of fungal polygalacturonases from tomato. Plant Mol Biol 25:607–617. doi:10.1007/BF00029600
• Toubart P, Desiderio A, Salvi G, Cervone F, Daroda L, De Lorenzo G (1992) Cloning and characterization of the gene encoding the endopolygalacturonase-inhibiting protein (PGIP) of Phaseolus vulgaris L. Plant J 2:367–373
• van der Hoorn RAL, Wulff BBH, Rivas S, Durrant MC, van der Ploeg A, de Wit PJGM (2005) Structure–function analysis of cf-9, a receptor-like protein with extracytoplasmic leucine-rich repeats. Plant Cell 17:1000–1015. doi:10.1105/tpc.104.028118
• Wulff BBH, Thomas CM, Smoker M, Grant M, Jones JDG (2001) Domain swapping and gene shuffling identify sequences required for induction of an Avr-dependent hypersensitive response by the tomato Cf-4 and Cf-9 proteins. Plant Cell 13:255–272
• Yao C, Conway WS, Ren R, Smith D, Ross GS, Sams CE (1999) Gene encoding polygalacturonase inhibitor in apple fruit is developmentally regulated and activated by wounding and fungal infection. Plant Mol Biol 39:1231–1241. doi:10.1023/A:1006155723059

Uwagi

rekord w opracowaniu