First detection and molecular characterization of Echinococcus equinus in a mule in Turkey

• Autorzy: Simsek S. , Cevik A.
• Języki publikacji: EN

Abstrakty

EN

Cystic echinococcosis is a zoonotic disease with a cosmopolital distribution. It is caused by the larval stages (metacestodes) of the parasite Echinococcus granulosus which infects different animal species. In this report, we present a case of E. granulosus infection in a mule and molecular characterization of the cyst. For this purpose parasite material was collected from the liver of a necropsied mule. DNA was isolated and PCR amplification of mitochondrial 12S rRNA as well as partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) genes were performed. Six unilocular cysts, filled with clear fluid were found in the liver and spleen. All cysts were found to be fertile. The 12S rRNA-PCR did not yield any band while mt-CO1-PCR yielded a 446 bp sized amplification product. Sequence corresponding to mt-CO1 gene was identical to a sequence reported for E. equinus (formerly G4) (Genbank accession number: KC953029). This is the first record of E. equinus as a cause of cystic echinococcosis in a mule in Turkey.

Słowa kluczowe

EN

Echinococcus granulosus; mule; Turkey; cystic echinococcosis; zoonotic disease; parasite; genotyping; polymerase chain reaction; cytochrome C oxidase subunit 1

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2014
• Tom: 59
• Numer: 4
• Opis fizyczny: p.773-777,fig.,ref.

Twórcy

autor

Simsek S.

• Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, 23119, Elazig, Turkey

autor

Cevik A.

• Department of Pathology, Faculty of Veterinary Medicine, University of Firat, 23119, Elazig, Turkey

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