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2012 | 34 | 4 |

Tytuł artykułu

Characterization and expression analysis of the maize RING-H12 finger protein gene ZmXERICO responsive to plant hormones and abiotic stresses

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Really interesting new gene (RING)-H2 proteins are widely represented in plants and play important roles in the regulation of many developmental processes as well as in plant–environment interactions. In the present report, a full-length cDNA encoding a putative RING-H2 protein with high sequence homology to Arabidopsis thaliana RING-H2 gene XERICO, designated as ZmXERICO, was isolated from maize. The full-length cDNA of ZmXERICO contained a 435 bp open reading frame encoding a precursor protein of 145 amino acid residues. Comparative and bioinformatics analyses revealed that ZmXERICO showed a high degree of homology with RING-H2 proteins and contained the transmembrane domain (TM) and RING-H2 motif. In our study, ZmXERICO was analyzed for expression profiles in various organs (root, stem, leaf, stamen, pistil and seed) and responding to exogenous hormone treatment and abiotic stresses using real-time PCR. The results showed that the expression of ZmXERICO was different regulated after treated by gibberellins acid-3 (GA₃) and GA biosynthetic inhibitor paclobutrazol between in light and darkness. Moreover, the expression of ZmXERICO under different abiotic stresses was analyzed at different time points. The ZmXERICO was up-regulated in salt, drought, abscisic acid and cold stress. These expression results suggested that ZmXERICO played an important role in plant hormones signal pathways and light signal pathways, and that ZmXERICO might be involved in plant defense system against environmental stresses in maize.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

34

Numer

4

Opis fizyczny

p.1529-1535,fig.,ref.

Twórcy

autor
  • Key Laboratories of Crop Genetics and Physiology of the Jiangsu Province and Plant Functional Genomics of the Ministry of Education, College of Bioscience and Biotechnology, Yangzhou University, 225009 Yangzhou, Jiangsu, People's Republic of China
autor
  • Key Laboratories of Crop Genetics and Physiology of the Jiangsu Province and Plant Functional Genomics of the Ministry of Education, College of Bioscience and Biotechnology, Yangzhou University, 225009 Yangzhou, Jiangsu, People's Republic of China
autor
  • Key Laboratories of Crop Genetics and Physiology of the Jiangsu Province and Plant Functional Genomics of the Ministry of Education, College of Bioscience and Biotechnology, Yangzhou University, 225009 Yangzhou, Jiangsu, People's Republic of China
autor
  • Key Laboratories of Crop Genetics and Physiology of the Jiangsu Province and Plant Functional Genomics of the Ministry of Education, College of Bioscience and Biotechnology, Yangzhou University, 225009 Yangzhou, Jiangsu, People's Republic of China
autor
  • Key Laboratories of Crop Genetics and Physiology of the Jiangsu Province and Plant Functional Genomics of the Ministry of Education, College of Bioscience and Biotechnology, Yangzhou University, 225009 Yangzhou, Jiangsu, People's Republic of China
autor
  • Key Laboratories of Crop Genetics and Physiology of the Jiangsu Province and Plant Functional Genomics of the Ministry of Education, College of Bioscience and Biotechnology, Yangzhou University, 225009 Yangzhou, Jiangsu, People's Republic of China

Bibliografia

  • Bopopi JM, Vandeputte OM, Himanen K, Mol A, Vaessen Q, El Jaziri M, Baucher M (2010) Ectopic expression of PtaRHE1, encoding a poplar RING-H2 protein with E3 ligase activity, alters plant development and induces defence-related responses. J Exp Bot 61:297–310
  • de Lucas M, Daviere JM, Rodriguez-Falcon M, Pontin M, Iglesias-Pedraz JM, Lorrain S, Fankhauser C, Blazquez MA, Titarenko E, Prat S (2008) A molecular framework for light and gibberellin control of cell elongation. Nature 451:480–484
  • Du QL, Cui WZ, Zhang CH, Yu DY (2010) GmRFP1 encodes a previously unknown RING-type E3 ubiquitin ligase in Soybean (Glycine max). Mol Biol Rep 37:685–693
  • Feng S, Martinez C, Gusmaroli G, Wang Y, Zhou J, Wang F, Chen L, Yu L, Iglesias-Pedraz JM, Kircher S, Schafer E, Fu X, Fan LM, Deng XW (2008) Coordinated regulation of Arabidopsis thaliana development by light and gibberellins. Nature 451:475–479
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  • Kam J, Gresshoff P, Shorter R, Xue GP (2007) Expression analysis of RING zinc finger genes from Triticum aestivum and identification of TaRZF70 that contains four RING-H2 domains and differentially responds to water deficit between leaf and root. Plant Sci 173:650–659
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  • Ko JH, Yang SH, Han KH (2006) Upregulation of an Arabidopsis RING-H2 gene, XERICO, confers drought tolerance through increased abscisic acid biosynthesis. Plant Journal 47:343–355
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  • Piskurewicz U, Jikumaru Y, Kinoshita N, Nambara E, Kamiya Y, Lopez-Molina L (2008) The gibberellic acid signaling repressor RGL2 inhibits Arabidopsis seed germination by stimulating abscisic acid synthesis and ABI5 activity. Plant Cell 20:2729–2745
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Bibliografia

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