Effect of meta-topolin on the shoot multiplication of pear rootstock OHF-333 (Pyrus communis L.)

• Autorzy: Dimitrova N. , Nacheva L. , Berova M.

Warianty tytułu

PL

Wpływ meta-topoliny na mikropropagację podkładki gruszy OHF-333 (Pyrus communis L.)

• Języki publikacji: EN

Abstrakty

EN

This study assesses the effect of meta-topolin (mT), an aromatic natural cytokinin, on micropropagation of pear rootstock OHF-333 (Pyrus сommunis L.). Cultures were incubated in a growth chamber under controlled conditions. An in vitro culture was maintained on a modified culture medium Murashige and Skoog [1962] supplemented with meta-topolin (0 μM, 3 μM, 6 μM, 9 μM or 12 μM). After three weeks’ growth the parameters and the physiological and biochemical analysis were investigated. The results of this study suggest that the in vitro culture in the absence of cytokinins does not provide a practical solution for efficient multiplication of pear rootstocks. A good multiplication rate and high quality shoots were found at 6–9 μM mT treatment. The use of meta-topolin resulted in improvement of the leaf gas exchange and low content of phenols, as well as in the total antioxidant activity. Hence the cytokinin meta-topolin in concentrations of 6–9 μM was selected as an optimum cytokinin level in the multiplication of pear rootstock OHF-333.

PL

W badaniu oceniono wpływ naturalnej, aromatycznej cytokininy meta-topoliny (mT) na mikropropagację podkładek gruszy OHF-333 (Pyrus сommunis L.). Hodowlę eksplantatów przeprowadzono w komorze hodowlanej w kontrolowanych warunkach. Eksplantaty wykładano na pożywkę Murashige i Skooga [1962] uzupełnioną meta-topoliną (0 μM, 3 μM , 6 μM, 9 μM lub 12 μM). Po trzech tygodniach określono parametry wzrostu, wykonano analizy fizjologiczne i biochemiczne. Wyniki tego badania wskazują, że w hodowli in vitro brak cytokininy nie zapewnia praktycznego rozwiązania dla skutecznego mikrorozmnażania podkładek gruszy. Zastosowanie 6–9 μM meta-topoliny spowodowało polepszenie współczynnika rozmnażania oraz otrzymanie pędów o wysokiej jakości. Użycie 6–9 μM meta-topoliny spowodowało również poprawę wymiany gazowej liści. Dlatego cytokinina meta-topolina w stężeniu 6–9 μM została wskazana jako optymalny poziom cytokinin przy mikrorozmnażaniu podkładek gruszy OHF-333.

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2016
• Tom: 15
• Numer: 2
• Opis fizyczny: p.43-53,fig.,ref.

Twórcy

autor

Dimitrova N.

• Department of Plant Physiology and Biochemistry, Faculty of Agriculture, Agricultural University, Plovdiv, Bulgaria

autor

Nacheva L.

• Agricultural University, Plovdiv, Bulgaria
• Fruit Growing Institute, Plovdiv, Bulgaria

autor

Berova M.

• Agricultural University, Plovdiv, Bulgaria

Bibliografia

• Aremu, A., Bairu, M., Dolezal, K., Finnie, J., Van Staden, J. (2012). Topolins: A panacea to plant tissue culture challenges? Plant Celi Tiss Organ Cult., 108, 1-16.
• Bairu, M., Stirk, W., Dolezal, K., Van Staden, J. (2007). Optimizing the micropropagation protocol for the endangered Aloe polyphylla'. can meta-topolin and its derivatives serve as replacement for benzyladenine and zeatin? Plant Celi Tiss Organ Cult., 90, 15-23.
• Bairu, M., Stirk, W., Dolezal, K., Van Staden, J. (2008). The role of topolins in micropropagation and somaclonal variation of banana cultivars ‘Williams’ and ‘Grand Naine’ (Musa spp. AAA). Plant Celi Tiss Organ Cult., 95, 373-379.
• Baroja-Fernandez, E., Aguirreolea, J., Martienkova, H., Hanus, J., Strnad, M. (2002). Aromatic cytokinins in micropropagated potato plants. Plant Physiol. Biochem., 40, 217-224.
• Beadle, C. (1993). Growth analysis. Photosynthesis and production in a changing environment. Springer-Sci.+Buisness Media, B.V., 36-46.
• Bell, R., Reed, B. (2002). In vitro tissue culture of pear: advances in techniąues for micropropagation and germplasm preservation. Acta Hort., 596, 412-418.
• Bogaert, I., Van Cauter, S., Werbrouck, S., Dolezal, K. (2006). New aromatic cytokinins can make the difference. Acta Hortic., 725, 265-270.
• Caers, M., Vendrig, C. (1986). Benzyladenine effects on the development of the photosynthetic apparatus in Zea mays: Studies on photosynthetic activity, enzymes and (etio) chloroplast ultrastructure. Physiol. Plant., 66, 685-691.
• Fletcher, A., Kallidumbil, V., Steele, P. (1982). Stimulation of chlorophyll synthesis in cucumber using cucumber cotyledons. Plant Physiol., 69, 937-939.
• Fujiwara, K., Kira, S., Kozai, T. (1992). Time course of CO₂ exchange of potato cultures in vitro with different sucrose concentration in the culture medium. J. Agr. Met., 48, 49-56.
• Harrar, Y., Bellec, Y., Bellini, C., Faure, J-D. (2003). Hormonal control of celi proliferation requires PASTICCINO genes. Plant Physiol., 132, 1217-1227.
• Howell, S., H., Lali, S., Che, P. (2003). Cytokinins and shoot development. Trends Plant Sci., 8, 453-459.
• Huetteman, C., Preece, J. (1993). Thidiazuron - a potent cytokinin for woody plant tissue culture. Plant Celi Tiss. Org., 33, 105-119.
• Jones, L., Martinkovat, H., Stmad, M., Hanke, D. (1996). Occurrence of aromatic cytokinins in oil palm (Elaeis guineensis Jacq.). J. Plant Growth Reguł., 15, 39-49.
• Kaminek, M., Vanek, T., Kalendova, A., Pilar, J. (1987). Effect of two cytokinins on production of stem cuttings by stock plants of Euphorbia pulcherrima Wild. and Gerbera jamesonii Hook. Sci. Hortic., 33, 281-289.
• Kubalakova, M., Stmad, M. (1992). The effect of aromatic cytokinins (populins) on micropropagation and regeneration in vitro. Biol. Plant., 34, 578-579.
• Lambert, P., Westwood, M. (1987). Pear rootstocks. In: Rootstocks for fruit crops, Rom, R., Carlson, R. (eds). Willey, New York.
• Leshem, B., Shaley, D., Izhar, S. (1988). Cytokinin as an inducer of hyperhydricity in melon. Ann. Bot., 61, 255-260.
• Lichtenthaler, H., Wellburn, A. (1983). Determination of total carotenoids and chlorophyll a and b of leaf extracts in different solvents. Biochem. Soc. Trans., 603, 591-592.
• Magyar-Tabori, K., Dobranszki, J., Teixeira da Silvą J., Bulley, S., Hudak, I. (2010). The role of cytokinins in shoot organogenesis in apple. Plant Celi Tiss Organ Cult., 101, 251-267.
• Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bio assays for tobacco tissue cultures. Physiol. Plant., 15, 473-497.
• Parthier, B. (1979). Phytohormones and chloroplast development. Biochem. Physiol. Pflanzen., 174, 173-214.
• Podwyszyńska, M., Węgrzynowicz-Lesiak, E., Dolezal, K., Krekule, K., Strnad, M., Saniewski, M. (2012). New cytokinin - meta-methoxytopolins in micropropagation of Cotinus coggygria Scop. ‘Royal Purple’. Prop. Omam. Plants., 12, 4, 220-228.
• Pospisilovą J., Catsky, J., Sestak, Z. (1996). Photosynthesis in plants cultured in vitro. In: Handbook of photosynthesis, Pessarali, M. (ed.). Marcel Dekker, NY.
• Roels, S., Escalona, M., Cejas, I., Noceda, C., Rodriguez, R., Canal, M.J., Sandoval, J., Debergh, P. (2005). Optimization of plantain (Musa AAB) micropropagation by temporary immersion system. Plant Celi Tiss Organ Cult., 82, 57-66.
• Rossi, M., Giussani, E., Morelli, R., Lo Scalzo, R., Nani, C., Torreggiani, D. (2003). Effect of fruit blanching on phenolics and radical scavenging activity of highbush blueberryjuice. Food Res. Intern., 36, 999-1005.
• Ruzic, D., Vujovic, T., Nikolic, D., Cerovic, R. (2011). In vitro growth responses of the ‘Pyrodwarf pear rootstock to cytokinin types. Romanian Biotechnol. Lett., 16, 5, 6630-6637.
• Salvi, N., George, L., Eapen, S. (2002). Micropropagation and field evaluation of micropropagated plants ofturmeric. Plant Celi Tissue Organ Cult., 68, 143-151.
• Sengul, M., Yildiz, H., Gungor, N., Cetin, B., Eser, Z., Ercisli, S. (2009). Total phenolic content, antioxidant and antimicrobial activities of some medicinal plants. J. Pharm Sci., 22, 1, 102-6.
• Singleton, V., Rossi, J. (1965). Colorunetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Amer. J. Enol. Viticult., 16, 144-58.
• Smulders, M., De Klerk, G. (2011). Epigenetics in plant tissue culture. Plant Growth Reguł., 63, 137-146.
• Strnad, M., Hanus, J., Vanek, T., Kaminek, M., Ballantine, J., Fussell, B., Hanke, D. (1997). Metatopolin, a highly active aromatic cytokinin from poplar leaves (Populus canadensis Moench, cv. Robusta). Phytochemistry, 45, 213-218.
• Tarkowska D., Dolezal, K., Tarkowski, P., Astot, C., Hołub, J., Fuksova, K., Schmulling, T., Sandberg, G., Strnad, M. (2003). Identification of new aromatic cytokinins in Arabidopsis thaliana and Populus canadensis leaves by LC-(+)ESIMS and capillary liquid chromatography frit-fast atom bombardment mass spectrometry. Physiol. Plant., 117(4), 579-590.
• Teramoto, H., Momotani, E., Tsuji, H. (1993). Benzyladenine-induced changes in the translatable mRNA population in excised cucumber cotyledons. Physiol. Plant., 87, 584-591.
• Van der Zwet, T., Beer, S. (1995). Fire blight - Its Nature, Prevention and Control. U.S. Department of Agriculture, Agricultural Information BulletinNo. 631.
• Werbrouck, S. (2010). Merits and drawbacks of new aromatic cytokinins in plant tissue culture. ActaHortic., 865,103-108.
• Werbrouck, S., Stmad, M., Van Onckelen, H., Debergh, P. (1996). Meta-topolin, an alternative to benzyladenine in tissue culture? Physiol. Plant., 98, 291-297.
• Wertheim, S. (2002). Rootstocks for European pear: a Review. Acta Hortic., 596, 299-309.
• Wojtania, A., Gabryszewska, E. (2001). Effect of cytokinins and amino acids on multiplication of Pelargonium cultivars. Acta Soc. Bot. Pol., 70, 203-207.
• Yen, G., Chen, H. (1995). Antioxidant activity of various tea extracts in relation to their antimutagenicity. J. Agric. Food Chem., 43, 1, 27-32.