Molecular evidence of Anaplasma phagocytophilum and Babesia microti co-infections in Ixodes ricinus ticks in central-eastern region of Poland

• Autorzy: Sytykiewicz H. , Karbowiak G. , Hapunik J. , Szpechcinski A. , Supergan-Marwicz M. , Golawska S. , Sprawka I. , Czerniewicz P.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to elucidate the distribution of Anaplasma phagocytophilum and Babesia microti co-infection in Ixodes ricinus populations within the central-eastern region of Poland. The prevalence of analysed tick-borne human pathogens in single and polymicrobial infections in I. ricinus ticks were analysed using the conventional and nested PCR techniques. A total number of 1,123 questing tick individuals (291 females, 267 males and 565 nymphs) were collected at different ecosystems (municipal parks, suburban forests, and woodlands). In the presented study, 95 samples of ticks (8.5%) were infected with A. phagocytophilum, 3.1% (n=35) with B. microti, whereas the co-existence status of these human pathogens was detected in 1.8% (n=20) of all tested samples. It has been demonstrated that the prevalence of co-infection status was the highest among females of I. ricinus (11 samples, 3.8%), whereas the lowest within tested nymphs (5 samples, 0.9%). Ticks collected at city parks in Warsaw and suburban areas of this town characterized the highest prevalence of co-infections (3.3 and 4.8%, respectively). Furthermore, it was established that co-infection rates of ticks inhabiting woodlands within Kampinos National Park and Nadbużański Landscape Park were similar and reached the levels of 1.4% (n=5) and 1.1% (n=4), respectively.

• Wydawca: -
• Czasopismo: Annals of Agricultural and Environmental Medicine
• Rocznik: 2012
• Tom: 19
• Numer: 1
• Opis fizyczny: p.45-49,fig.,ref.

Twórcy

autor

Sytykiewicz H.

• University of Natural Sciences and Humanities, Siedlce, Poland

autor

Karbowiak G.

• W. Stefański Institute of Parasitology of the Polish Academy of Sciences, Warsaw, Poland

autor

Hapunik J.

• W. Stefański Institute of Parasitology of the Polish Academy of Sciences, Warsaw, Poland

autor

Szpechcinski A.

• Institute of Tuberculosis and Lung Disease, Warsaw, Poland

autor

Supergan-Marwicz M.

• Institute of Tuberculosis and Lung Disease, Warsaw, Poland

autor

Golawska S.

• University of Natural Sciences and Humanities, Siedlce, Poland

autor

Sprawka I.

• University of Natural Sciences and Humanities, Siedlce, Poland

autor

Czerniewicz P.

• University of Natural Sciences and Humanities, Siedlce, Poland

Bibliografia

• 1. Angelakis E, Billeter SA, Breitschwerdt EB, Chomel BB, Raoult D. Potential for tick-borne bartonelloses. Emerg Infect Dis. 2010; 16:385-391.
• 2. Castellaw AH, Chenney EF, Varela-Stokes AS. Tick-borne disease agents in various wildlife from Mississippi. Vector Borne Zoonotic Dis. 2011,11(4): 439-442.
• 3. Daniel M, Materna J, Hőnig V, Metelka L, Danielová V, Harčarik J, Kligrová S, Grubhoffer L. Vertical distribution of the tick Ixodes ricinusand tick-borne pathogens in the northern Moravian mountainscorrelated with climate warming (Jeseníky Mts., Czech Republic).Cent Eur J Public Health. 2009; 17: 139-145.
• 4. Lempereur L, De Cat A, Caron Y, Madder M, Claerebout E, Saegerman C, Losson B. First molecular evidence of potentially zoonotic Babesiamicroti and Babesia sp. EU1 in Ixodes ricinus ticks in Belgium. VectorBorne Zoonotic Dis. 2011; 11: 125-130.
• 5. Severinsson K, Jaenson TG, Pettersson J, Falk K, Nilsson K. Detection and prevalence of Anaplasma phagocytophilum and Rickettsia helveticain Ixodes ricinus ticks in seven study areas in Sweden. Parasit Vectors2010, 3:66, DOI: 10.1186/1756-3305-3-66.
• 6. Tokarz R, Jain K, Bennett A, Briese T, Lipkin WI. Assessment of polymicrobial infections in ticks in New York State. Vector BorneZoonotic Dis. 2010; 10: 217-221.
• 7. Doudier B, Olano J, Parola P, Brouqui P. Factors contributing to emergence of Ehrlichia and Anaplasma spp. as human pathogens. VetParasitol. 2010; 167: 149-159.
• 8. Heyman P, Cochez C, Hofhuis A, van der Giessen J, Sprong H, Porter SR, Losson B, Saegerman C, Donoso-Mantke O, Niedrig M, Papa A.A clear and present danger: tick-borne diseases in Europe. Expert RevAnti Infect Ther. 2010; 8: 33-50.
• 9. Reye AL, Hübschen JM, Sausy A, Muller CP. Prevalence and seasonality of tick-borne pathogens in questing Ixodes ricinus ticks fromLuxembourg. Appl Environ Microbiol. 2010; 76: 2923-2931.
• 10. Blaschitz M, Narodoslavsky-Gfoller M, Kanzler M, Stanek G, Walochnik J. Babesia species occurring in Austrian Ixodes ricinusticks. Appl Environ Microbiol. 2008; 74: 4841-4846.
• 11. Hildebrandt A, Pauliks K, Sachse S, Straube E. Coexistence of Borrelia spp. and Babesia spp. in Ixodes ricinus ticks in middle Germany. VectorBorne Zoonotic Dis. 2010; 10: 831-837.
• 12. Karbowiak G. Zoonotic reservoir of Babesia microti in Poland. Pol J Microbiol. 2004; 53(Suppl.): 61-65.
• 13. Sytykiewicz H, Pucyk A. Detection of Babesia sp. DNA in common tick population occuring at Kampinos National Park. In: Buczek A,Błaszak C (Eds): Stawonogi – środowisko, patogeny i żywiciele. Wyd.Koliber, Lublin 2007; 147‑152.
• 14. Siński E. Wpływ konfekcji u kleszczy (Ixodidae) na transmisję mikropasożytów krwi. Wiad Parazytol. 2009; 55: 341-347.
• 15. Halos L, Bord S, Cotté V, Gasqui P, Abrial D, Barnouin J, Boulouis H-J, Vayssier-Taussat M, Vourc’h G. Ecological factors characterizing theprevalence of bacterial tick-borne pathogens in Ixodes ricinus ticks inpastures and woodlands. Appl Environ Microbiol. 2010; 76: 4413-4420.
• 16. Dietrich F, Schmidgen T, Maggi RG, Richter D, Matuschka F-R, Vonthein R, Breitschwerdt EB, Kempf VAJ. Prevalence of Bartonellahenselae and Borrelia burgdorferi sensu lato DNA in Ixodes ricinusticks in Europe. Appl Environ Microbiol. 2010; 76: 1395-1398.
• 17. Aktas M, Vatansever Z, Altay K, Aydin MF, Dumanli N. Molecularevidence for Anaplasma phagocytophilum in Ixodes ricinus from Turkey.Trans R Soc Trop Med Hyg. 2010; 104: 10-15.
• 18. Franke J, Fritzsch J, Tomaso H, Straube E, Dorn W, Hildebrandt A. Coexistence of pathogens in host-seeking and feeding ticks within a single natural habitat in central Germany. Appl Environ Microbiol. 2010; 76: 6829-6836.
• 19. Nonaka E, Ebel GD, Wearing HJ. Persistence of pathogens with short infectious periods in seasonal tick populations: the relative importanceof three transmission routes. PLoS ONE 2010; 5: e11745.
• 20. Torina A, Alongi A, Scimeca S, Vicente J, Caracappa S, de la Fuente J. Prevalence of tick-borne pathogens in ticks in Sicily. Transbound EmergDis. 2010; 57: 46-48.
• 21. Václav R, Ficová M, Prokop P, Betáková T. Associations between coinfection prevalence of Borrelia lusitaniae, Anaplasma sp., andRickettsia sp. in hard ticks feeding on reptile hosts. Microb Ecol. 2011;61: 245-253.
• 22. Grzeszczuk A, Stańczak J, Kubica-Biernat B. Serological and molecular evidence of human granulocytic ehrlichiosis focus in the BiałowieżaPrimeval Forest (Puszcza Białowieska), northeastern Poland. Eur JClin Microbiol Infect Dis. 2002; 21: 6-11.
• 23. Stańczak J, Gable RM, Kruminis-Łozowska W, Carewicz M, Kubica- Biernat B. Ixodes ricinus as a vector of Borrelia burgdorferi sensulato, Anaplasma phagocytophilum and Babesia microti in urban andsuburban forests. Ann Agric Environ Med. 2004; 11: 109-114.
• 24. Persing DH, Mathiesen D, Marshall WF, Telford SR, Spielman A, Thomford JW, Conrad PA. Detection of Babesia microti by polymerasechain reaction. J Clin Microbiol. 1992; 30: 2097-2103.
• 25. Masuzawa T, Kharitonenkov IG, Okamoto Y, Fukui T, Ohashi N. Prevalence of Anaplasma phagocytophilum and its coinfection withBorrelia afzelii in Ixodes ricinus and Ixodes persulcatus ticks inhabitingTver Province (Russia) – a sympatric region for both tick species. J MedMicrobiol. 2008; 57: 986-991.
• 26. Wójcik-Fatla A, Szamańska J, Wdowiak L, Buczek A, Dutkiewicz J. Coincidence of three pathogens (Borrelia burgdorferi sensu lato,Anaplasma phagocytophilum and Babesia microti) in Ixodes ricinusticks in the Lublin macroregion. Ann Agric Environ Med. 2009; 16:151-158.
• 27. Halos L, Jamal T, Maillard R, Beugnet F, Le Menach A, Boulouis H-J, Vayssier-Taussat M. Evidence of Bartonella sp. in questing adult andnymphal Ixodes ricinus ticks from France and co-infection with Borreliaburgdorferi sensu lato and Babesia sp. Vet Res. 2005; 36: 79-87.
• 28. Hildebrandt A, Straube E, Neubauer H, Schmoock G. Coxiella burnetii and co-infections in Ixodes ricinus ticks in central Germany. VectorBorne Zoonotic Dis. 2010, DOI:10.1089/vbz.2010.0180 [ahead of print].
• 29. Koči J, Movila A, Taragel’ová V, Toderas I, Uspenskaia I, Derdáková M, Labuda M. First report of Anaplasma phagocytophilum and itscoinfections with Borrelia burgdorferi sensu lato in Ixodes ricinus ticks(Acari: Ixodidae) from Republic of Moldova. Exp Appl Acarol. 2007;41: 147-152.
• 30. Nieto NC, Foley JE. Meta-analysis of coinfection and coexposure with Borrelia burgdorferi and Anaplasma phagocytophilum in humans,domestic animals, wildlife, and Ixodes ricinus-complex ticks. VectorBorne Zoonotic Dis. 2009; 9: 93-101.
• 31. Swanson SJ, Neitzel D, Reed KD, Belongia EA. Coinfections acquired from Ixodes ticks. Clin Microbiol Rev. 2006; 19: 708-727.
• 32. Toledo Á, Olmeda AS, Escudero R, Jado I, Valcárcel F, Casado-Nistal MA, Rodríguez-Vargas M , Gil H, Anda P. Tick-borne zoonotic bacteria in ticks collected from central Spain. Am J Trop Med Hyg. 2009; 81:67-74.
• 33. Vennestrøm J, Egholm H, Jensen PM. Occurrence of multiple infections with different Borrelia burgdorferi genospecies in Danish Ixodes ricinusnymphs. Parasitol Int. 2008; 57: 32-37.
• 34. Vorou RM, Papavassiliou VG, Tsiodras S. Emerging zoonoses and vector-borne infections affecting humans in Europe. Epidemiol Infect.2007; 135: 1231-1247.
• 35. Zygner W, Bąska P, Wiśniewski M, Wędrychowicz H. The molecular evidence of Babesia microti in hard ticks removed from dogs in Warsaw(central Poland). Pol J Microbiol. 2010; 59: 95-97.
• 36. Hildebrandt A, Hunfeld KP, Baier M, Krumbholz A, Sachse S, Lorenzen T, Kiehntopf M, Fricke HJ, Straube E. First confimed autochtonous caseof human Babesia microti infection in Europe. Eur J Clin MicrobiolInfect Dis. 2007; 26: 595-601.
• 37. Becker CA, Bouju-Albert A, Jouglin M, Chauvin A, Malandrin L. Natural transmission of zoonotic Babesia spp. by Ixodes ricinus ticks.Emerg Infect Dis. 2009; 15; 320-322.
• 38. Bonnet S, Brisseau N, Hermouet A, Jouglin M, Chauvin A. Experimental in vitro transmission of Babesia sp. (EU1) by Ixodes ricinus. Vet Res.2009; 40: 21-28.
• 39. Casati S, Sager H, Gern L, Piffaretti JC. Presence of potentially pathogenic Babesia sp. for human in Ixodes ricinus in Switzerland.Ann Agric Environ Med. 2006; 13: 65-70.
• 40. Häselbarth K, Tenter AM, Brade V, Krieger G, Hunfeld KP. First case of human babesiosis in Germany-clinical presentation and molecularcharacterisation of the pathogen. Int J Med Microbiol. 2007; 297: 197-204.
• 41. Hunfeld KP, Brade V. Zoonotic Babesia: possibly emerging pathogens to be considered for tick-infested humans in Central Europe. Int J MedMicrobiol. 2004; 293(Suppl. 37): 93-103.

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