Charakterystyka szczepów chorobotwórczych Yersinia ruckeri i ich znaczenie w immunoprofilaktyce pstrąga tęczowego

• Autorzy: Pajdak J. , Terech-Majewska E. , Platt-Samoraj A. , Schulz P. , Siwicki A.K. , Szweda W.

Warianty tytułu

EN

Characterization of pathogenic Yersinia ruckeri strains and their importance in rainbow trout immunoprophylaxis

• Języki publikacji: PL

Abstrakty

EN

Due to the wide range of distribution, tendency to stationary persistence and high susceptibility of salmonid fish, vaccination against yersiniosis is an important part of prophylactic programs in many trout farms. The use of autovaccines prepared on the basis of isolates from a given fish farm is considered to be more effective than the use of commercial preparations. The aim of the study was to analyze cases of the disease in 2015 and to try to explain the failure of implemented prophylactic program based on autovaccination. Y. ruckeri strains isolated from one fish farm, from disease outbreaks in 2006, 2008 and 2015, as well as strains used for vaccine production were analyzed. Biotype evaluation, biochemical properties using API 20E assays, serotyping, and molecular identification were performed. All analyzed strains belonged to biotype 2. Two different profiles were obtained in API 20E tests. The first was obtained for vaccine strains and from disease cases in 2006 and 2008. The second was obtained for the strain isolated from the disease case in 2015. None of the profiles have been interpreted as Y. ruckeri in the APIweb. The identification of bacteria was confirmed by PCR. Disease strains and two vaccine strains belonged to the O1 serotype. Two of the vaccine strains have been qualified as O5 serotype. The obtained results indicate that the disease outbreak in 2015 was due to the emergence of a strain with increased pathogenicity and the use of an autovaccine based on strains belonging to the O5 serotype, considered to be less pathogenic. Immunization with the use of the O5 serotype does not provide complete protection against infection with other serotypes, so that fish remained susceptible to O1 serotype, which caused the disease in 2015. The results suggest the need for extending the diagnostics of strains used for the production of autovaccines.

Słowa kluczowe

PL

ryby; pstrag teczowy; choroby zwierzat; jersynioza; immunoprofilaktyka; czynniki chorobotworcze; Yersinia ruckeri; szczepy bakteryjne; serotypy; charakterystyka; przelamanie odpornosci poszczepiennej

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2017
• Tom: 73
• Numer: 09
• Opis fizyczny: s.579-584,fot.,bibliogr.

Twórcy

autor

Pajdak J.

• Katedra Epizootiologii, Wydział Medycyny Weterynaryjnej, Uniwersytet Warmińsko-Mazurski w Olsztynie, ul.Oczapowskiego 13, 10-718 Olsztyn

autor

Terech-Majewska E.

• Katedra Epizootiologii, Wydział Medycyny Weterynaryjnej, Uniwersytet Warmińsko-Mazurski w Olsztynie, ul.Oczapowskiego 13, 10-718 Olsztyn

autor

Platt-Samoraj A.

• Katedra Epizootiologii, Wydział Medycyny Weterynaryjnej, Uniwersytet Warmińsko-Mazurski w Olsztynie, ul.Oczapowskiego 13, 10-718 Olsztyn

autor

Schulz P.

• Katedra Mikrobiologii i Immunologii, Wydział Medycyny Weterynaryjnej, Uniwersytet Warmińsko-Mazurski w Olsztynie, ul.Oczapowskiego 13, 10-718 Olsztyn

autor

Siwicki A.K.

• Katedra Mikrobiologii i Immunologii, Wydział Medycyny Weterynaryjnej, Uniwersytet Warmińsko-Mazurski w Olsztynie, ul.Oczapowskiego 13, 10-718 Olsztyn

autor

Szweda W.

• Katedra Epizootiologii, Wydział Medycyny Weterynaryjnej, Uniwersytet Warmińsko-Mazurski w Olsztynie, ul.Oczapowskiego 13, 10-718 Olsztyn

Bibliografia

• Altinok I., Grizzle J. M., Liu Z. J.: Detection of Yersinia ruckeri in rainbow trout blood by use of the olymerase chain reaction. Dis. Aquat. Org. 2001, 44, 29-34.
• Altun S., Onuk E. E., Ciftci A., Duman M., Buyukekiz A. G.: Determination of phenotypic, serotypic and genetic diversity and antibiotyping of Yersinia ruckeri isolated from rainbow trout. Kafkas Univ. Vet. Fak. Derg. 2013, 19, 2, 225-232.
• Bastardo A., Ravelo C., Romalde J. L.: A polyphasic approach to study the intraspecific diversity of Yersinia ruckeri strains isolated from recent outbreaks in salmonid culture. Vet. Microbiol. 2012, 160, 176-182.
• Bastardo A., Ravelo C., Romalde J. L.: Phylogeography of Yersinia ruckeri reveals effects of past evolutionary events on the current strain distribution and explains variations in the global transmission of enteric redmouth (ERM) disease. Front. Microbiol. 2015, 6, 1198.
• Calvez S., Gantelet H., Blanc G., Douet D. G., Daniel P.: Yersinia ruckeri Biotypes 1 and 2 in France: presence and antibiotic susceptibility. Dis. Aquat. Org. 2014, 109, 117-126.
• Davies R. L.: O-serotyping of Yersinia-ruckeri with special emphasis on european isolates. Vet. Microbiol. 1990, 22, 299-307.
• Fernandez L., Lopez J. R., Secades P., Menendez A., Marquez I., Guijarro J. A.: In vitro and in vivo studies of the Yrp1 protease from Yersinia ruckeri and its role in protective immunity against enteric red mouth disease of salmonids. Appl. Environ. Microb. 2003, 69, 7328-7335.
• Fernandez L., Mendez J., Guijarro J. A.: Molecular virulence mechanisms of the fish pathogen Yersinia ruckeri. Vet. Microbiol. 2007, 125, 1-10.
• Fernandez L., Prieto M., Guijarro J. A.: The iron- and temperature-regulated haemolysinYhlA is a virulence factor of Yersinia ruckeri. Microbiology 2007, 153, 483-489.
• Grawiński E.: The occurrence of redmouth disease in the rainbow trout (Salmo gairdneri Richardson). Med. Weter. 1990, 46, 183-185.
• Huang Y. D., Jung A., Schafer W. J., Mock D., Michael G. B., Runge M., Schwarz S., Steinhagen D.: Analysis of Yersinia ruckeri strains isolated from trout farms in northwest Germany. Dis. Aquat. Org. 2015, 116, 243-249.
• Kumar G., Menanteau-Ledouble S., Saleh M., El-Matbouli M.: Yersinia ruckeri, the causative agent of enteric redmouth disease in fish. Vet. Res. 2015, 46, 103.
• Ormsby M. J., Caws T., Burchmore R., Wallis T., Verner-Jeffreys D. W., Davies R. L.: Yersinia ruckeri isolates recovered from diseased Atlantic salmon (Salmo salar) in Scotland are more diverse than those from Rainbow trout (Oncorhynchus mykiss) and represent distinct subpopulations. Appl. Environ. Microbiol. 2016, 82, 5785-5794.
• Pękala A.: Charakterystyka fenotypowa i genotypowa krajowych izolatów Yersinia ruckeriw aspekcie ich patogenności dla ryb. Praca doktorska, Zakład Chorób Ryb, PIWet-PIB, Puławy 2008.
• Pękala A., Antychowicz J.: Jersinioza ryb łososiowatych – charakterystyka czynnika etiologicznego I metody jego identyfikacji. Med. Weter. 2010, 66, 299-301.
• Pękala A., Antychowicz J.: Jersinioza ryb łososiowatych – epizootiologia choroby, metody jej zwalczania. Med. Weter. 2010, 66, 374-377.
• Pękala A., Kozinska A., Antychowicz J.: Serological variation among polish isolates of Yersinia ruckeri. Bull. Vet. Inst. Pulawy 2010, 54, 305-308.
• Rucker R. R.: Redmouth disease of rainbow trout (Salmo gairdneri). Bull. Internat. Off. Epizoot. 1966, 65, 825-830.
• Seker E., Karahan M., Ispir U., Cetinkaya B., Saglam N., Sarieyyupoglu M.: Investigation of Yersinia ruckeri infection in Rainbow trout (Oncorhynchusmykiss Walbaum 1792) farms by polymerase chain reaction (PCR) and bacteriological culture. Kafkas Univ. Vet. Fak. Derg. 2012, 18, 913-916.
• Tinsley J. W., Austin D. A., Lyndon A. R., Austin B.: Novel non-motile phenotypes of Yersinia ruckeri suggest expansion of the current clonal complex theory. J. Fish Dis. 2011, 34, 311-317.
• Tobback E., Decostere A., Hermans K., Haesebrouck F., Chiers K.: Yersinia ruckeri infections in salmonid fish. J. Fish Dis. 2007, 30, 257-268.

Identyfikatory

DOI

10.21521/mw.5767